Summary

Summary {data-width=650}

Manhattan plot

manhattan_plot

manhattan_plot

QQ plot

qq_plot

qq_plot

AF plot

af_plot

af_plot

P-Z plot

pz_plot

pz_plot

beta_std plot

beta_std_plot

beta_std_plot

Metadata

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}

LDSC

*********************************************************************
* LD Score Regression (LDSC)
* Version 1.0.1
* (C) 2014-2019 Brendan Bulik-Sullivan and Hilary Finucane
* Broad Institute of MIT and Harvard / MIT Department of Mathematics
* GNU General Public License v3
*********************************************************************
Call: 
./ldsc.py \
--h2 /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/ukb-d-import/processed/ukb-d-O02/ukb-d-O02.vcf.gz \
--ref-ld-chr /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/ \
--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/ukb-d-import/processed/ukb-d-O02/ldsc.txt \
--w-ld-chr /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/ 

Beginning analysis at Mon Nov 25 17:20:57 2019
Reading summary statistics from /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/ukb-d-import/processed/ukb-d-O02/ukb-d-O02.vcf.gz ...
Read summary statistics for 9470567 SNPs.
Dropped 7006 SNPs with duplicated rs numbers.
Reading reference panel LD Score from /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/[1-22] ...
Read reference panel LD Scores for 1290028 SNPs.
Removing partitioned LD Scores with zero variance.
Reading regression weight LD Score from /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/[1-22] ...
Read regression weight LD Scores for 1290028 SNPs.
After merging with reference panel LD, 1272672 SNPs remain.
After merging with regression SNP LD, 1272672 SNPs remain.
Using two-step estimator with cutoff at 30.
Total Observed scale h2: 0.0006 (0.0012)
Lambda GC: 1.0025
Mean Chi^2: 1.007
Intercept: 1.0029 (0.0061)
Ratio: 0.4171 (0.8743)
Analysis finished at Mon Nov 25 17:22:32 2019
Total time elapsed: 1.0m:34.18s

QC metrics

Metrics

Metrics

{
    "af_correlation": 0.9387,
    "inflation_factor": 0.9864,
    "mean_EFFECT": -2.4243e-07,
    "n": 361194,
    "n_snps": 9470567,
    "n_clumped_hits": 0,
    "n_p_sig": 0,
    "n_mono": 0,
    "n_ns": 1048542,
    "n_mac": 0,
    "is_snpid_unique": false,
    "n_miss_EFFECT": 0,
    "n_miss_SE": 0,
    "n_miss_PVAL": 0,
    "n_miss_AF": 0,
    "n_miss_AF_reference": 189685,
    "n_est": 361020.1222,
    "ratio_se_n": 0.9998,
    "mean_diff": -4.3381e-06,
    "ratio_diff": 25.0485,
    "sd_y_est1": 0.055,
    "sd_y_est2": 0.055,
    "r2_sum1": 0,
    "r2_sum2": 0,
    "r2_sum3": 0,
    "r2_sum4": 0,
    "ldsc_nsnp_merge_refpanel_ld": 1272672,
    "ldsc_nsnp_merge_regression_ld": 1272672,
    "ldsc_observed_scale_h2_beta": 0.0006,
    "ldsc_observed_scale_h2_se": 0.0012,
    "ldsc_intercept_beta": 1.0029,
    "ldsc_intercept_se": 0.0061,
    "ldsc_lambda_gc": 1.0025,
    "ldsc_mean_chisq": 1.007,
    "ldsc_ratio": 0.4143
}

Flags

name value
af_correlation FALSE
inflation_factor FALSE
n FALSE
is_snpid_non_unique TRUE
mean_EFFECT_nonfinite FALSE
mean_EFFECT_05 FALSE
mean_EFFECT_01 FALSE
mean_chisq FALSE
n_p_sig FALSE
miss_EFFECT FALSE
miss_SE FALSE
miss_PVAL FALSE
ldsc_ratio FALSE
ldsc_intercept_beta FALSE
n_clumped_hits FALSE
r2_sum1 FALSE
r2_sum2 FALSE
r2_sum3 FALSE
r2_sum4 FALSE

Definitions

General metrics

  • af_correlation: Correlation coefficient between AF and AF_reference.
  • inflation_factor (lambda): Genomic inflation factor.
  • mean_EFFECT: Mean of EFFECT size.
  • n: Maximum value of reported sample size across all SNPs, \(n\).
  • n_clumped_hits: Number of clumped hits.
  • n_snps: Number of SNPs
  • n_p_sig: Number of SNPs with pvalue below 5e-8.
  • n_mono: Number of monomorphic (MAF == 1 or MAF == 0) SNPs.
  • n_ns: Number of SNPs with nonsense values:
    • alleles other than A, C, G or T.
    • P-values < 0 or > 1.
    • negative or infinite standard errors (<= 0 or = Infinity).
    • infinite beta estimates or allele frequencies < 0 or > 1.
  • n_mac: Number of cases where MAC (\(2 \times N \times MAF\)) is less than 6.
  • is_snpid_unique: true if the combination of ID REF ALT is unique and therefore no duplication in snpid.
  • n_miss_<*>: Number of NA observations for <*> column.

se_n metrics

  • n_est: Estimated sample size value, \(\widehat{n}\).
  • ratio_se_n: \(\texttt{ratio_se_n} = \frac{\sqrt{\widehat{n}}}{\sqrt{n}}\). We expect ratio_se_n to be 1. When it is not 1, it implies that the trait did not have a variance of 1, the reported sample size is wrong, or that the SNP-level effective sample sizes differ markedly from the reported sample size.
  • mean_diff: \(\texttt{mean_diff} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta_j}{\texttt{n_snps}}\), mean difference between the standardised beta, predicted from P-values, and the observed beta. The difference should be very close to zero if trait has a variance of 1.
    • \(\widehat{\beta_j^{std}} = \sqrt{\frac{{z}_j^2 / ({z}_j^2 + n -2)}{2 \times {MAF}_j \times (1 - {MAF}_j)}} \times sign({z}_j)\),
    • \({z}_j = \frac{\beta_j}{{se}_j}\),
    • and \(\beta_j\) is the reported effect size.
  • ratio_diff: \(\texttt{ratio_diff} = |\frac{\texttt{mean_diff}}{\texttt{mean_diff2}}|\), absolute ratio between the mean of diff and the mean of diff2 (expected difference between the standardised beta predicted from P-values, and the standardised beta derived from the observed beta divided by the predicted SD; NOT reported). The ratio should be close to 1. If different from 1, then implies that the betas are not in a standard deviation scale.
    • \(\texttt{mean_diff2} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta^{\prime}_j}{\texttt{n_snps}}\)
    • \(\beta^{\prime}_j = \frac{\beta_j}{\widehat{\texttt{sd2}}_{y}}\)
  • sd_y_est1: The standard deviation for the trait inferred from the reported sample size, median standard errors for the SNP-trait assocations and SNP variances.
    • \(\widehat{\texttt{sd1}}_{y} = \frac{\sqrt{n} \times median({se}_j)}{C}\),
    • \(C = median(\frac{1}{\sqrt{2 \times {MAF}_j \times (1 - {MAF}_j)}})\),
    • and \({se}_j\) is the reported standard error.
  • sd_y_est2: The standard deviation for the trait inferred from the reported sample size, Z statistics for the SNP-trait effects (beta/se) and allele frequency.
    • \(\widehat{\texttt{sd2}}_{y} = median(\widehat{sd_j})\),
    • \(\widehat{sd_j} = \frac{\beta_j}{\widehat{\beta_j^{std}}}\),

r2 metrics

Sum of variance explained, calculated from the clumped top hits sample.

  • r2_sum<*>: r2 statistics under various assumptions
    • 1: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var1}}}\), \(\texttt{var1} = 1\).
    • 2: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var2}}}\), \(\texttt{var2} = {\widehat{\texttt{sd1}}_{y}}^2\),
    • 3: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var3}}}\), \(\texttt{var3} = {\widehat{\texttt{sd2}}_{y}}^2\),
    • 4: \(r^2 = \sum_j{\frac{F_j}{F_j + n - 2}}\), \(F = \frac{\beta_j^2}{{se}_j^2}\).

LDSC metrics

Metrics from LD regression

  • ldsc_nsnp_merge_refpanel_ld: Number of remaining SNPs after merging with reference panel LD.
  • ldsc_nsnp_merge_regression_ld: Number of remaining SNPs after merging with regression SNP LD.
  • ldsc_observed_scale_h2_{beta,se} Coefficient value and SE for total observed scale h2.
  • ldsc_intercept_{beta,se}: Coefficient value and SE for intercept. Intercept is expected to be 1.
  • ldsc_lambda_gc: Lambda GC statistics.
  • ldsc_mean_chisq: Mean \(\chi^2\) statistics.
  • ldsc_ratio: \(\frac{\texttt{ldsc_intercept_beta} - 1}{\texttt{ldsc_mean_chisq} - 1}\), the proportion of the inflation in the mean \(\chi^2\) that the LD Score regression intercepts ascribes to causes other than polygenic heritability. The value of ratio should be close to zero, though in practice values of 0.1-0.2 are not uncommon, probably due to sample/reference LD Score mismatch or model misspecification (e.g., low LD variants have slightly higher \(h^2\) per SNP).

Flags

When a metric needs attention, the flag should return TRUE.

  • af_correlation: abs(af_correlation) < 0.7.
  • inflation_factor: inflation_factor > 1.2.
  • n: n (max reported sample size) < 10000.
  • is_snpid_non_unique: NOT is_snpid_unique.
  • mean_EFFECT_nonfinite: mean(EFFECT) is NA, NaN, or Inf.
  • mean_EFFECT_05: abs(mean(EFFECT)) > 0.5.
  • mean_EFFECT_01: abs(mean(EFFECT)) > 0.1.
  • mean_chisq: ldsc_mean_chisq > 1.3 or ldsc_mean_chisq < 0.7.
  • n_p_sig: n_p_sig > 1000.
  • miss_<*>: n_miss_<*> / n_snps > 0.01.
  • ldsc_ratio: ldsc_ratio > 0.5
  • ldsc_intercept_beta: ldsc_intercept_beta > 1.5
  • n_clumped_hits: n_clumped_hits > 1000
  • r2_sum<*>: r2_sum<*> > 0.5

Plots

  • Manhattan plot
    • Red line: \(-log_{10}^{5 \times 10^{-8}}\)
    • Blue line: \(-log_{10}^{5 \times 10^{-5}}\)
  • QQ plot
  • AF plot
  • P-Z plot
  • beta_std plot: Scatter plot between \(\widehat{\beta_j^{std}}\) and \(\beta_j\)

Diagnostics

Details

Summary stats

skim_type skim_variable n_missing complete_rate character.min character.max character.empty character.n_unique character.whitespace numeric.mean numeric.sd numeric.p0 numeric.p25 numeric.p50 numeric.p75 numeric.p100 numeric.hist
character ID 0 1.0000000 3 94 0 9464099 0 NA NA NA NA NA NA NA NA
character REF 0 1.0000000 1 98 0 46274 0 NA NA NA NA NA NA NA NA
character ALT 0 1.0000000 1 342 0 29373 0 NA NA NA NA NA NA NA NA
numeric CHROM 0 1.0000000 NA NA NA NA NA 9.080559e+00 6.173770e+00 1.00000e+00 4.000000e+00 8.000000e+00 1.300000e+01 2.300000e+01 ▇▅▅▂▂
numeric POS 0 1.0000000 NA NA NA NA NA 7.870106e+07 5.611155e+07 3.02000e+02 3.241581e+07 6.956809e+07 1.147204e+08 2.492297e+08 ▇▆▅▂▁
numeric EFFECT 0 1.0000000 NA NA NA NA NA -2.000000e-07 2.771000e-04 -2.60030e-03 -1.378000e-04 -1.700000e-06 1.343000e-04 2.898600e-03 ▁▁▇▁▁
numeric SE 0 1.0000000 NA NA NA NA NA 2.439000e-04 1.326000e-04 1.06500e-04 1.412000e-04 1.857000e-04 3.097000e-04 7.635000e-04 ▇▂▂▁▁
numeric PVAL 0 1.0000000 NA NA NA NA NA 5.015234e-01 2.883431e-01 3.00000e-07 2.520444e-01 5.029224e-01 7.510736e-01 1.000000e+00 ▇▇▇▇▇
numeric PVAL_ztest 0 1.0000000 NA NA NA NA NA 5.015229e-01 2.883434e-01 3.00000e-07 2.520437e-01 5.029217e-01 7.510727e-01 1.000000e+00 ▇▇▇▇▇
numeric AF 0 1.0000000 NA NA NA NA NA 2.672661e-01 2.626964e-01 1.12410e-02 4.873300e-02 1.675330e-01 4.245640e-01 9.887590e-01 ▇▂▂▁▁
numeric AF_reference 189685 0.9799711 NA NA NA NA NA 2.666103e-01 2.534952e-01 0.00000e+00 5.591050e-02 1.821090e-01 4.193290e-01 1.000000e+00 ▇▃▂▂▁
numeric N 0 1.0000000 NA NA NA NA NA 3.611940e+05 0.000000e+00 3.61194e+05 3.611940e+05 3.611940e+05 3.611940e+05 3.611940e+05 ▁▁▇▁▁

Head and tail

CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
1 692794 rs530212009 CA C 0.0001075 0.0002269 0.6357861 0.6357860 0.1106400 0.1894970 361194
1 693731 rs12238997 A G 0.0001659 0.0002144 0.4389136 0.4389158 0.1158300 0.1417730 361194
1 707522 rs371890604 G C 0.0002473 0.0002410 0.3048063 0.3048058 0.0973034 0.1293930 361194
1 717587 rs144155419 G A -0.0003155 0.0005751 0.5832342 0.5832338 0.0156880 0.0045926 361194
1 730087 rs148120343 T C -0.0000511 0.0002987 0.8641221 0.8641220 0.0564602 0.0127796 361194
1 731718 rs142557973 T C 0.0001235 0.0002033 0.5435018 0.5435011 0.1217380 0.1543530 361194
1 732032 rs61770163 A C 0.0001678 0.0002169 0.4392179 0.4392172 0.1211710 0.1555510 361194
1 734349 rs141242758 T C 0.0001205 0.0002034 0.5535948 0.5535945 0.1209650 0.1525560 361194
1 749963 rs529266287 T TAA -0.0001946 0.0002005 0.3318677 0.3318667 0.8697420 0.7641770 361194
1 751343 rs28544273 T A 0.0002546 0.0001986 0.1998798 0.1998800 0.1229160 0.2426120 361194
CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
23 154923311 rs141127553 C T 0.0000058 0.0002349 0.9803340 0.9803342 0.0561667 0.0309934 361194
23 154923374 rs111332691 T A 0.0001689 0.0002581 0.5129405 0.5129384 0.0447862 0.0116556 361194
23 154925045 rs509981 C T 0.0001150 0.0001242 0.3543399 0.3543401 0.2456060 0.3634440 361194
23 154925895 rs538470 C T 0.0000900 0.0001271 0.4788208 0.4788219 0.2419150 0.3634440 361194
23 154927581 rs644138 G A 0.0001035 0.0001168 0.3757751 0.3757744 0.3021620 0.4635760 361194
23 154929412 rs557132 C T 0.0001071 0.0001242 0.3888320 0.3888313 0.2454590 0.3568210 361194
23 154929637 rs35185538 CT C 0.0001326 0.0001297 0.3066677 0.3066666 0.2296970 0.3011920 361194
23 154929952 rs4012982 CAA C 0.0001200 0.0001307 0.3586287 0.3586298 0.2394250 0.3165560 361194
23 154930230 rs781880 A G 0.0001054 0.0001242 0.3961366 0.3961381 0.2458690 0.3618540 361194
23 154930487 rs781879 T A -0.0000361 0.0004262 0.9325310 0.9325310 0.0195623 0.1263580 361194

bcf preview

1   692794  rs530212009 CA  C   .   PASS    AF=0.11064  ES:SE:LP:AF:SS:ID   0.000107452:0.000226884:0.196689:0.11064:361194:1_692794_CA_C
1   693731  rs12238997  A   G   .   PASS    AF=0.11583  ES:SE:LP:AF:SS:ID   0.000165934:0.000214378:0.357621:0.11583:361194:rs12238997
1   707522  rs371890604 G   C   .   PASS    AF=0.0973034    ES:SE:LP:AF:SS:ID   0.000247315:0.000241005:0.515976:0.0973034:361194:rs371890604
1   717587  rs144155419 G   A   .   PASS    AF=0.015688 ES:SE:LP:AF:SS:ID   -0.000315538:0.000575099:0.234157:0.015688:361194:rs144155419
1   730087  rs148120343 T   C   .   PASS    AF=0.0564602    ES:SE:LP:AF:SS:ID   -5.1109e-05:0.000298657:0.0634249:0.0564602:361194:rs148120343
1   731718  rs58276399  T   C   .   PASS    AF=0.121738 ES:SE:LP:AF:SS:ID   0.00012352:0.000203316:0.264799:0.121738:361194:rs58276399
1   732032  rs61770163  A   C   .   PASS    AF=0.121171 ES:SE:LP:AF:SS:ID   0.000167757:0.000216876:0.35732:0.121171:361194:rs61770163
1   734349  rs141242758 T   C   .   PASS    AF=0.120965 ES:SE:LP:AF:SS:ID   0.000120506:0.000203426:0.256808:0.120965:361194:rs141242758
1   749963  rs529266287 T   TAA .   PASS    AF=0.869742 ES:SE:LP:AF:SS:ID   -0.000194601:0.000200545:0.479035:0.869742:361194:rs529266287
1   751343  rs28544273  T   A   .   PASS    AF=0.122916 ES:SE:LP:AF:SS:ID   0.000254602:0.000198614:0.699231:0.122916:361194:rs28544273
1   751488  rs200141114 G   GA  .   PASS    AF=0.142712 ES:SE:LP:AF:SS:ID   9.72159e-05:0.000196319:0.207283:0.142712:361194:rs200141114
1   751756  rs28527770  T   C   .   PASS    AF=0.123031 ES:SE:LP:AF:SS:ID   0.000258504:0.000198337:0.715673:0.123031:361194:rs28527770
1   753405  rs3115860   C   A   .   PASS    AF=0.87089  ES:SE:LP:AF:SS:ID   -0.000198089:0.000193273:0.515127:0.87089:361194:rs3115860
1   753425  rs3131970   T   C   .   PASS    AF=0.875472 ES:SE:LP:AF:SS:ID   -0.000223159:0.000196476:0.591696:0.875472:361194:rs3131970
1   753541  rs2073813   G   A   .   PASS    AF=0.128631 ES:SE:LP:AF:SS:ID   0.000200272:0.000193758:0.520979:0.128631:361194:rs2073813
1   754105  rs12184325  C   T   .   PASS    AF=0.0363613    ES:SE:LP:AF:SS:ID   0.000135797:0.000351522:0.155358:0.0363613:361194:rs12184325
1   754182  rs3131969   A   G   .   PASS    AF=0.870484 ES:SE:LP:AF:SS:ID   -0.000188164:0.000193072:0.481788:0.870484:361194:rs3131969
1   754192  rs3131968   A   G   .   PASS    AF=0.870589 ES:SE:LP:AF:SS:ID   -0.000178288:0.00019316:0.448543:0.870589:361194:rs3131968
1   754334  rs3131967   T   C   .   PASS    AF=0.870481 ES:SE:LP:AF:SS:ID   -0.000188382:0.000193065:0.482551:0.870481:361194:rs3131967
1   755890  rs3115858   A   T   .   PASS    AF=0.870563 ES:SE:LP:AF:SS:ID   -0.000193832:0.000192725:0.502323:0.870563:361194:rs3115858
1   756434  rs61768170  G   C   .   PASS    AF=0.125985 ES:SE:LP:AF:SS:ID   0.000223717:0.00019665:0.592995:0.125985:361194:rs61768170
1   756604  rs3131962   A   G   .   PASS    AF=0.870127 ES:SE:LP:AF:SS:ID   -0.000197691:0.000192261:0.517359:0.870127:361194:rs3131962
1   757640  rs3115853   G   A   .   PASS    AF=0.869414 ES:SE:LP:AF:SS:ID   -0.000195038:0.000192086:0.508735:0.869414:361194:rs3115853
1   757734  rs4951929   C   T   .   PASS    AF=0.870281 ES:SE:LP:AF:SS:ID   -0.0002017:0.000192427:0.530838:0.870281:361194:rs4951929
1   757936  rs4951862   C   A   .   PASS    AF=0.870287 ES:SE:LP:AF:SS:ID   -0.000201938:0.000192441:0.531628:0.870287:361194:rs4951862
1   758144  rs3131956   A   G   .   PASS    AF=0.870295 ES:SE:LP:AF:SS:ID   -0.000201397:0.000192447:0.529697:0.870295:361194:rs3131956
1   758626  rs3131954   C   T   .   PASS    AF=0.870739 ES:SE:LP:AF:SS:ID   -0.000195507:0.000192937:0.507368:0.870739:361194:rs3131954
1   759293  rs10157329  T   A   .   PASS    AF=0.0988442    ES:SE:LP:AF:SS:ID   0.000331827:0.000224204:0.857398:0.0988442:361194:rs10157329
1   759837  rs3115851   T   A   .   PASS    AF=0.874635 ES:SE:LP:AF:SS:ID   -0.00021688:0.000195812:0.571802:0.874635:361194:rs3115851
1   761732  rs2286139   C   T   .   PASS    AF=0.864027 ES:SE:LP:AF:SS:ID   -0.000229624:0.000192023:0.634943:0.864027:361194:rs2286139
1   761752  rs1057213   C   T   .   PASS    AF=0.869425 ES:SE:LP:AF:SS:ID   -0.000191795:0.000193887:0.491388:0.869425:361194:rs1057213
1   762273  rs3115849   G   A   .   PASS    AF=0.866371 ES:SE:LP:AF:SS:ID   -0.000199612:0.000193926:0.518085:0.866371:361194:rs3115849
1   762485  rs12095200  C   A   .   PASS    AF=0.0987831    ES:SE:LP:AF:SS:ID   0.000330087:0.000232166:0.809408:0.0987831:361194:rs12095200
1   762589  rs3115848   G   C   .   PASS    AF=0.871556 ES:SE:LP:AF:SS:ID   -0.000205838:0.000196136:0.531706:0.871556:361194:rs3115848
1   762592  rs3131950   C   G   .   PASS    AF=0.871556 ES:SE:LP:AF:SS:ID   -0.000205837:0.000196136:0.531703:0.871556:361194:rs3131950
1   762601  rs3131949   T   C   .   PASS    AF=0.871555 ES:SE:LP:AF:SS:ID   -0.000205827:0.00019614:0.531654:0.871555:361194:rs3131949
1   762632  rs3131948   T   A   .   PASS    AF=0.871929 ES:SE:LP:AF:SS:ID   -0.000214227:0.000196251:0.560647:0.871929:361194:rs3131948
1   764191  rs7515915   T   G   .   PASS    AF=0.125562 ES:SE:LP:AF:SS:ID   0.000228248:0.000196406:0.610503:0.125562:361194:rs7515915
1   766007  rs61768174  A   C   .   PASS    AF=0.105428 ES:SE:LP:AF:SS:ID   0.000253433:0.000213815:0.627265:0.105428:361194:rs61768174
1   766105  rs2519015   T   A   .   PASS    AF=0.855376 ES:SE:LP:AF:SS:ID   -0.000132119:0.000191951:0.308683:0.855376:361194:rs2519015
1   768116  rs376645387 A   AGTTTT  .   PASS    AF=0.838358 ES:SE:LP:AF:SS:ID   2.19637e-05:0.000189106:0.0421352:0.838358:361194:rs376645387
1   768253  rs2977608   A   C   .   PASS    AF=0.763351 ES:SE:LP:AF:SS:ID   -9.91203e-05:0.000152195:0.288301:0.763351:361194:rs2977608
1   768448  rs12562034  G   A   .   PASS    AF=0.105921 ES:SE:LP:AF:SS:ID   -5.35316e-05:0.000209688:0.0977262:0.105921:361194:rs12562034
1   769138  rs59306077  CAT C   .   PASS    AF=0.128938 ES:SE:LP:AF:SS:ID   0.000210352:0.000193659:0.556908:0.128938:361194:rs762168062
1   769223  rs60320384  C   G   .   PASS    AF=0.128608 ES:SE:LP:AF:SS:ID   0.00020792:0.00019348:0.548922:0.128608:361194:rs60320384
1   769963  rs7518545   G   A   .   PASS    AF=0.105213 ES:SE:LP:AF:SS:ID   -8.34159e-05:0.000211272:0.159286:0.105213:361194:rs7518545
1   770886  rs371458725 G   A   .   PASS    AF=0.104268 ES:SE:LP:AF:SS:ID   -7.39177e-05:0.00021296:0.137559:0.104268:361194:rs371458725
1   771410  rs2519006   C   T   .   PASS    AF=0.830106 ES:SE:LP:AF:SS:ID   -0.000136348:0.000188523:0.328334:0.830106:361194:rs2519006
1   771823  rs2977605   T   C   .   PASS    AF=0.87012  ES:SE:LP:AF:SS:ID   -0.000186039:0.000192614:0.476109:0.87012:361194:rs2977605
1   771967  rs59066358  G   A   .   PASS    AF=0.128668 ES:SE:LP:AF:SS:ID   0.000202764:0.000193389:0.531031:0.128668:361194:rs59066358