Summary

Summary {data-width=650}

Manhattan plot

manhattan_plot

manhattan_plot

QQ plot

qq_plot

qq_plot

AF plot

af_plot

af_plot

P-Z plot

pz_plot

pz_plot

beta_std plot

beta_std_plot

beta_std_plot

Metadata

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}

LDSC

*********************************************************************
* LD Score Regression (LDSC)
* Version 1.0.1
* (C) 2014-2019 Brendan Bulik-Sullivan and Hilary Finucane
* Broad Institute of MIT and Harvard / MIT Department of Mathematics
* GNU General Public License v3
*********************************************************************
Call: 
./ldsc.py \
--h2 /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/ukb-d-import/processed/ukb-d-N31/ukb-d-N31.vcf.gz \
--ref-ld-chr /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/ \
--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/ukb-d-import/processed/ukb-d-N31/ldsc.txt \
--w-ld-chr /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/ 

Beginning analysis at Mon Nov 25 17:46:39 2019
Reading summary statistics from /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/ukb-d-import/processed/ukb-d-N31/ukb-d-N31.vcf.gz ...
Read summary statistics for 9417336 SNPs.
Dropped 6934 SNPs with duplicated rs numbers.
Reading reference panel LD Score from /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/[1-22] ...
Read reference panel LD Scores for 1290028 SNPs.
Removing partitioned LD Scores with zero variance.
Reading regression weight LD Score from /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/[1-22] ...
Read regression weight LD Scores for 1290028 SNPs.
After merging with reference panel LD, 1271833 SNPs remain.
After merging with regression SNP LD, 1271833 SNPs remain.
Using two-step estimator with cutoff at 30.
Total Observed scale h2: -0.0005 (0.0012)
Lambda GC: 1.0109
Mean Chi^2: 1.0111
Intercept: 1.0145 (0.0064)
Ratio: 1.3015 (0.5736)
Analysis finished at Mon Nov 25 17:48:14 2019
Total time elapsed: 1.0m:34.34s

QC metrics

Metrics

Metrics

{
    "af_correlation": 0.9383,
    "inflation_factor": 1.0157,
    "mean_EFFECT": -8.9301e-07,
    "n": 361194,
    "n_snps": 9417336,
    "n_clumped_hits": 0,
    "n_p_sig": 0,
    "n_mono": 0,
    "n_ns": 1045071,
    "n_mac": 0,
    "is_snpid_unique": false,
    "n_miss_EFFECT": 0,
    "n_miss_SE": 0,
    "n_miss_PVAL": 0,
    "n_miss_AF": 0,
    "n_miss_AF_reference": 188878,
    "n_est": 361113.4106,
    "ratio_se_n": 0.9999,
    "mean_diff": -0,
    "ratio_diff": 15.3934,
    "sd_y_est1": 0.0547,
    "sd_y_est2": 0.0547,
    "r2_sum1": 0,
    "r2_sum2": 0,
    "r2_sum3": 0,
    "r2_sum4": 0,
    "ldsc_nsnp_merge_refpanel_ld": 1271833,
    "ldsc_nsnp_merge_regression_ld": 1271833,
    "ldsc_observed_scale_h2_beta": "NA",
    "ldsc_observed_scale_h2_se": "NA",
    "ldsc_intercept_beta": 1.0145,
    "ldsc_intercept_se": 0.0064,
    "ldsc_lambda_gc": 1.0109,
    "ldsc_mean_chisq": 1.0111,
    "ldsc_ratio": 1.3063
}

Flags

name value
af_correlation FALSE
inflation_factor FALSE
n FALSE
is_snpid_non_unique TRUE
mean_EFFECT_nonfinite FALSE
mean_EFFECT_05 FALSE
mean_EFFECT_01 FALSE
mean_chisq FALSE
n_p_sig FALSE
miss_EFFECT FALSE
miss_SE FALSE
miss_PVAL FALSE
ldsc_ratio TRUE
ldsc_intercept_beta FALSE
n_clumped_hits FALSE
r2_sum1 FALSE
r2_sum2 FALSE
r2_sum3 FALSE
r2_sum4 FALSE

Definitions

General metrics

  • af_correlation: Correlation coefficient between AF and AF_reference.
  • inflation_factor (lambda): Genomic inflation factor.
  • mean_EFFECT: Mean of EFFECT size.
  • n: Maximum value of reported sample size across all SNPs, \(n\).
  • n_clumped_hits: Number of clumped hits.
  • n_snps: Number of SNPs
  • n_p_sig: Number of SNPs with pvalue below 5e-8.
  • n_mono: Number of monomorphic (MAF == 1 or MAF == 0) SNPs.
  • n_ns: Number of SNPs with nonsense values:
    • alleles other than A, C, G or T.
    • P-values < 0 or > 1.
    • negative or infinite standard errors (<= 0 or = Infinity).
    • infinite beta estimates or allele frequencies < 0 or > 1.
  • n_mac: Number of cases where MAC (\(2 \times N \times MAF\)) is less than 6.
  • is_snpid_unique: true if the combination of ID REF ALT is unique and therefore no duplication in snpid.
  • n_miss_<*>: Number of NA observations for <*> column.

se_n metrics

  • n_est: Estimated sample size value, \(\widehat{n}\).
  • ratio_se_n: \(\texttt{ratio_se_n} = \frac{\sqrt{\widehat{n}}}{\sqrt{n}}\). We expect ratio_se_n to be 1. When it is not 1, it implies that the trait did not have a variance of 1, the reported sample size is wrong, or that the SNP-level effective sample sizes differ markedly from the reported sample size.
  • mean_diff: \(\texttt{mean_diff} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta_j}{\texttt{n_snps}}\), mean difference between the standardised beta, predicted from P-values, and the observed beta. The difference should be very close to zero if trait has a variance of 1.
    • \(\widehat{\beta_j^{std}} = \sqrt{\frac{{z}_j^2 / ({z}_j^2 + n -2)}{2 \times {MAF}_j \times (1 - {MAF}_j)}} \times sign({z}_j)\),
    • \({z}_j = \frac{\beta_j}{{se}_j}\),
    • and \(\beta_j\) is the reported effect size.
  • ratio_diff: \(\texttt{ratio_diff} = |\frac{\texttt{mean_diff}}{\texttt{mean_diff2}}|\), absolute ratio between the mean of diff and the mean of diff2 (expected difference between the standardised beta predicted from P-values, and the standardised beta derived from the observed beta divided by the predicted SD; NOT reported). The ratio should be close to 1. If different from 1, then implies that the betas are not in a standard deviation scale.
    • \(\texttt{mean_diff2} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta^{\prime}_j}{\texttt{n_snps}}\)
    • \(\beta^{\prime}_j = \frac{\beta_j}{\widehat{\texttt{sd2}}_{y}}\)
  • sd_y_est1: The standard deviation for the trait inferred from the reported sample size, median standard errors for the SNP-trait assocations and SNP variances.
    • \(\widehat{\texttt{sd1}}_{y} = \frac{\sqrt{n} \times median({se}_j)}{C}\),
    • \(C = median(\frac{1}{\sqrt{2 \times {MAF}_j \times (1 - {MAF}_j)}})\),
    • and \({se}_j\) is the reported standard error.
  • sd_y_est2: The standard deviation for the trait inferred from the reported sample size, Z statistics for the SNP-trait effects (beta/se) and allele frequency.
    • \(\widehat{\texttt{sd2}}_{y} = median(\widehat{sd_j})\),
    • \(\widehat{sd_j} = \frac{\beta_j}{\widehat{\beta_j^{std}}}\),

r2 metrics

Sum of variance explained, calculated from the clumped top hits sample.

  • r2_sum<*>: r2 statistics under various assumptions
    • 1: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var1}}}\), \(\texttt{var1} = 1\).
    • 2: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var2}}}\), \(\texttt{var2} = {\widehat{\texttt{sd1}}_{y}}^2\),
    • 3: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var3}}}\), \(\texttt{var3} = {\widehat{\texttt{sd2}}_{y}}^2\),
    • 4: \(r^2 = \sum_j{\frac{F_j}{F_j + n - 2}}\), \(F = \frac{\beta_j^2}{{se}_j^2}\).

LDSC metrics

Metrics from LD regression

  • ldsc_nsnp_merge_refpanel_ld: Number of remaining SNPs after merging with reference panel LD.
  • ldsc_nsnp_merge_regression_ld: Number of remaining SNPs after merging with regression SNP LD.
  • ldsc_observed_scale_h2_{beta,se} Coefficient value and SE for total observed scale h2.
  • ldsc_intercept_{beta,se}: Coefficient value and SE for intercept. Intercept is expected to be 1.
  • ldsc_lambda_gc: Lambda GC statistics.
  • ldsc_mean_chisq: Mean \(\chi^2\) statistics.
  • ldsc_ratio: \(\frac{\texttt{ldsc_intercept_beta} - 1}{\texttt{ldsc_mean_chisq} - 1}\), the proportion of the inflation in the mean \(\chi^2\) that the LD Score regression intercepts ascribes to causes other than polygenic heritability. The value of ratio should be close to zero, though in practice values of 0.1-0.2 are not uncommon, probably due to sample/reference LD Score mismatch or model misspecification (e.g., low LD variants have slightly higher \(h^2\) per SNP).

Flags

When a metric needs attention, the flag should return TRUE.

  • af_correlation: abs(af_correlation) < 0.7.
  • inflation_factor: inflation_factor > 1.2.
  • n: n (max reported sample size) < 10000.
  • is_snpid_non_unique: NOT is_snpid_unique.
  • mean_EFFECT_nonfinite: mean(EFFECT) is NA, NaN, or Inf.
  • mean_EFFECT_05: abs(mean(EFFECT)) > 0.5.
  • mean_EFFECT_01: abs(mean(EFFECT)) > 0.1.
  • mean_chisq: ldsc_mean_chisq > 1.3 or ldsc_mean_chisq < 0.7.
  • n_p_sig: n_p_sig > 1000.
  • miss_<*>: n_miss_<*> / n_snps > 0.01.
  • ldsc_ratio: ldsc_ratio > 0.5
  • ldsc_intercept_beta: ldsc_intercept_beta > 1.5
  • n_clumped_hits: n_clumped_hits > 1000
  • r2_sum<*>: r2_sum<*> > 0.5

Plots

  • Manhattan plot
    • Red line: \(-log_{10}^{5 \times 10^{-8}}\)
    • Blue line: \(-log_{10}^{5 \times 10^{-5}}\)
  • QQ plot
  • AF plot
  • P-Z plot
  • beta_std plot: Scatter plot between \(\widehat{\beta_j^{std}}\) and \(\beta_j\)

Diagnostics

Details

Summary stats

skim_type skim_variable n_missing complete_rate character.min character.max character.empty character.n_unique character.whitespace numeric.mean numeric.sd numeric.p0 numeric.p25 numeric.p50 numeric.p75 numeric.p100 numeric.hist
character ID 0 1.0000000 3 94 0 9410939 0 NA NA NA NA NA NA NA NA
character REF 0 1.0000000 1 98 0 46098 0 NA NA NA NA NA NA NA NA
character ALT 0 1.0000000 1 342 0 29305 0 NA NA NA NA NA NA NA NA
numeric CHROM 0 1.0000000 NA NA NA NA NA 9.080624e+00 6.173791e+00 1.00000e+00 4.000000e+00 8.000000e+00 1.300000e+01 2.300000e+01 ▇▅▅▂▂
numeric POS 0 1.0000000 NA NA NA NA NA 7.869725e+07 5.611327e+07 3.02000e+02 3.241300e+07 6.956502e+07 1.147159e+08 2.492297e+08 ▇▆▅▂▁
numeric EFFECT 0 1.0000000 NA NA NA NA NA -9.000000e-07 2.744000e-04 -2.26790e-03 -1.385000e-04 -2.000000e-06 1.340000e-04 3.030000e-03 ▁▂▇▁▁
numeric SE 0 1.0000000 NA NA NA NA NA 2.404000e-04 1.291000e-04 1.05900e-04 1.402000e-04 1.838000e-04 3.045000e-04 7.589000e-04 ▇▂▂▁▁
numeric PVAL 0 1.0000000 NA NA NA NA NA 4.975831e-01 2.896506e-01 2.00000e-07 2.453079e-01 4.966449e-01 7.491146e-01 1.000000e+00 ▇▇▇▇▇
numeric PVAL_ztest 0 1.0000000 NA NA NA NA NA 4.975826e-01 2.896509e-01 2.00000e-07 2.453070e-01 4.966439e-01 7.491153e-01 9.999999e-01 ▇▇▇▇▇
numeric AF 0 1.0000000 NA NA NA NA NA 2.686030e-01 2.626345e-01 1.16063e-02 4.985780e-02 1.694370e-01 4.265820e-01 9.883940e-01 ▇▂▂▁▁
numeric AF_reference 188878 0.9799436 NA NA NA NA NA 2.679069e-01 2.534329e-01 0.00000e+00 5.730830e-02 1.839060e-01 4.211260e-01 1.000000e+00 ▇▃▂▂▁
numeric N 0 1.0000000 NA NA NA NA NA 3.611940e+05 0.000000e+00 3.61194e+05 3.611940e+05 3.611940e+05 3.611940e+05 3.611940e+05 ▁▁▇▁▁

Head and tail

CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
1 692794 rs530212009 CA C -0.0001048 0.0002255 0.6422055 0.6422061 0.1106400 0.1894970 361194
1 693731 rs12238997 A G -0.0000230 0.0002131 0.9138660 0.9138653 0.1158300 0.1417730 361194
1 707522 rs371890604 G C -0.0001814 0.0002396 0.4488053 0.4488032 0.0973034 0.1293930 361194
1 717587 rs144155419 G A 0.0003411 0.0005716 0.5507151 0.5507140 0.0156880 0.0045926 361194
1 730087 rs148120343 T C -0.0000142 0.0002969 0.9618900 0.9618899 0.0564602 0.0127796 361194
1 731718 rs142557973 T C -0.0000337 0.0002021 0.8674551 0.8674544 0.1217380 0.1543530 361194
1 732032 rs61770163 A C -0.0000417 0.0002156 0.8465931 0.8465932 0.1211710 0.1555510 361194
1 734349 rs141242758 T C -0.0000196 0.0002022 0.9227469 0.9227472 0.1209650 0.1525560 361194
1 749963 rs529266287 T TAA 0.0000505 0.0001993 0.8000639 0.8000634 0.8697420 0.7641770 361194
1 751343 rs28544273 T A -0.0000035 0.0001974 0.9856530 0.9856532 0.1229160 0.2426120 361194
CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
23 154923311 rs141127553 C T 0.0001278 0.0002335 0.5842463 0.5842479 0.0561667 0.0309934 361194
23 154923374 rs111332691 T A -0.0003099 0.0002566 0.2271031 0.2271023 0.0447862 0.0116556 361194
23 154925045 rs509981 C T 0.0000310 0.0001235 0.8017430 0.8017428 0.2456060 0.3634440 361194
23 154925895 rs538470 C T 0.0000340 0.0001263 0.7879397 0.7879395 0.2419150 0.3634440 361194
23 154927581 rs644138 G A 0.0000600 0.0001161 0.6054175 0.6054161 0.3021620 0.4635760 361194
23 154929412 rs557132 C T 0.0000327 0.0001235 0.7910993 0.7911000 0.2454590 0.3568210 361194
23 154929637 rs35185538 CT C 0.0000258 0.0001289 0.8411300 0.8411293 0.2296970 0.3011920 361194
23 154929952 rs4012982 CAA C 0.0000521 0.0001299 0.6882909 0.6882919 0.2394250 0.3165560 361194
23 154930230 rs781880 A G 0.0000407 0.0001235 0.7417644 0.7417640 0.2458690 0.3618540 361194
23 154930487 rs781879 T A -0.0004780 0.0004236 0.2591350 0.2591346 0.0195623 0.1263580 361194

bcf preview

1   692794  rs530212009 CA  C   .   PASS    AF=0.11064  ES:SE:LP:AF:SS:ID   -0.000104778:0.000225515:0.192326:0.11064:361194:1_692794_CA_C
1   693731  rs12238997  A   G   .   PASS    AF=0.11583  ES:SE:LP:AF:SS:ID   -2.30481e-05:0.000213084:0.0391175:0.11583:361194:rs12238997
1   707522  rs371890604 G   C   .   PASS    AF=0.0973034    ES:SE:LP:AF:SS:ID   -0.000181438:0.00023955:0.347942:0.0973034:361194:rs371890604
1   717587  rs144155419 G   A   .   PASS    AF=0.015688 ES:SE:LP:AF:SS:ID   0.000341085:0.000571628:0.259073:0.015688:361194:rs144155419
1   730087  rs148120343 T   C   .   PASS    AF=0.0564602    ES:SE:LP:AF:SS:ID   -1.41843e-05:0.000296854:0.0168746:0.0564602:361194:rs148120343
1   731718  rs58276399  T   C   .   PASS    AF=0.121738 ES:SE:LP:AF:SS:ID   -3.37272e-05:0.000202089:0.061753:0.121738:361194:rs58276399
1   732032  rs61770163  A   C   .   PASS    AF=0.121171 ES:SE:LP:AF:SS:ID   -4.17053e-05:0.000215568:0.0723253:0.121171:361194:rs61770163
1   734349  rs141242758 T   C   .   PASS    AF=0.120965 ES:SE:LP:AF:SS:ID   -1.96079e-05:0.000202198:0.0349174:0.120965:361194:rs141242758
1   749963  rs529266287 T   TAA .   PASS    AF=0.869742 ES:SE:LP:AF:SS:ID   5.04846e-05:0.000199335:0.0968753:0.869742:361194:rs529266287
1   751343  rs28544273  T   A   .   PASS    AF=0.122916 ES:SE:LP:AF:SS:ID   -3.54992e-06:0.000197415:0.00627595:0.122916:361194:rs28544273
1   751488  rs200141114 G   GA  .   PASS    AF=0.142712 ES:SE:LP:AF:SS:ID   3.95769e-05:0.000195134:0.0760947:0.142712:361194:rs200141114
1   751756  rs28527770  T   C   .   PASS    AF=0.123031 ES:SE:LP:AF:SS:ID   -6.61459e-06:0.000197141:0.0117827:0.123031:361194:rs28527770
1   753405  rs3115860   C   A   .   PASS    AF=0.87089  ES:SE:LP:AF:SS:ID   5.76017e-06:0.000192107:0.0105146:0.87089:361194:rs3115860
1   753425  rs3131970   T   C   .   PASS    AF=0.875472 ES:SE:LP:AF:SS:ID   -1.49164e-05:0.000195291:0.0272808:0.875472:361194:rs3131970
1   753541  rs2073813   G   A   .   PASS    AF=0.128631 ES:SE:LP:AF:SS:ID   -1.06556e-05:0.000192589:0.019598:0.128631:361194:rs2073813
1   754105  rs12184325  C   T   .   PASS    AF=0.0363613    ES:SE:LP:AF:SS:ID   -0.000364068:0.0003494:0.526627:0.0363613:361194:rs12184325
1   754182  rs3131969   A   G   .   PASS    AF=0.870484 ES:SE:LP:AF:SS:ID   1.81207e-06:0.000191907:0.00328436:0.870484:361194:rs3131969
1   754192  rs3131968   A   G   .   PASS    AF=0.870589 ES:SE:LP:AF:SS:ID   -1.3021e-06:0.000191995:0.00235635:0.870589:361194:rs3131968
1   754334  rs3131967   T   C   .   PASS    AF=0.870481 ES:SE:LP:AF:SS:ID   2.14551e-06:0.0001919:0.00389129:0.870481:361194:rs3131967
1   755890  rs3115858   A   T   .   PASS    AF=0.870563 ES:SE:LP:AF:SS:ID   1.11603e-05:0.000191563:0.0206602:0.870563:361194:rs3115858
1   756434  rs61768170  G   C   .   PASS    AF=0.125985 ES:SE:LP:AF:SS:ID   -9.133e-06:0.000195464:0.0164941:0.125985:361194:rs61768170
1   756604  rs3131962   A   G   .   PASS    AF=0.870127 ES:SE:LP:AF:SS:ID   1.99155e-05:0.000191101:0.0376311:0.870127:361194:rs3131962
1   757640  rs3115853   G   A   .   PASS    AF=0.869414 ES:SE:LP:AF:SS:ID   5.86437e-06:0.000190927:0.0107741:0.869414:361194:rs3115853
1   757734  rs4951929   C   T   .   PASS    AF=0.870281 ES:SE:LP:AF:SS:ID   1.60162e-05:0.000191266:0.029995:0.870281:361194:rs4951929
1   757936  rs4951862   C   A   .   PASS    AF=0.870287 ES:SE:LP:AF:SS:ID   1.60482e-05:0.00019128:0.0300546:0.870287:361194:rs4951862
1   758144  rs3131956   A   G   .   PASS    AF=0.870295 ES:SE:LP:AF:SS:ID   1.58165e-05:0.000191286:0.0296057:0.870295:361194:rs3131956
1   758626  rs3131954   C   T   .   PASS    AF=0.870739 ES:SE:LP:AF:SS:ID   1.00194e-05:0.000191773:0.018484:0.870739:361194:rs3131954
1   759293  rs10157329  T   A   .   PASS    AF=0.0988442    ES:SE:LP:AF:SS:ID   -1.95012e-05:0.000222851:0.0313919:0.0988442:361194:rs10157329
1   759837  rs3115851   T   A   .   PASS    AF=0.874635 ES:SE:LP:AF:SS:ID   1.07344e-05:0.000194631:0.0195344:0.874635:361194:rs3115851
1   761732  rs2286139   C   T   .   PASS    AF=0.864027 ES:SE:LP:AF:SS:ID   2.70616e-05:0.000190864:0.051954:0.864027:361194:rs2286139
1   761752  rs1057213   C   T   .   PASS    AF=0.869425 ES:SE:LP:AF:SS:ID   1.8964e-05:0.000192717:0.0354519:0.869425:361194:rs1057213
1   762273  rs3115849   G   A   .   PASS    AF=0.866371 ES:SE:LP:AF:SS:ID   2.61505e-05:0.000192756:0.0495938:0.866371:361194:rs3115849
1   762485  rs12095200  C   A   .   PASS    AF=0.0987831    ES:SE:LP:AF:SS:ID   -9.7991e-05:0.000230765:0.173211:0.0987831:361194:rs12095200
1   762589  rs3115848   G   C   .   PASS    AF=0.871556 ES:SE:LP:AF:SS:ID   8.78288e-06:0.000194952:0.0158932:0.871556:361194:rs3115848
1   762592  rs3131950   C   G   .   PASS    AF=0.871556 ES:SE:LP:AF:SS:ID   8.73432e-06:0.000194952:0.0158036:0.871556:361194:rs3131950
1   762601  rs3131949   T   C   .   PASS    AF=0.871555 ES:SE:LP:AF:SS:ID   8.68659e-06:0.000194956:0.0157153:0.871555:361194:rs3131949
1   762632  rs3131948   T   A   .   PASS    AF=0.871929 ES:SE:LP:AF:SS:ID   2.11566e-06:0.000195067:0.00377474:0.871929:361194:rs3131948
1   764191  rs7515915   T   G   .   PASS    AF=0.125562 ES:SE:LP:AF:SS:ID   -1.17998e-05:0.000195221:0.0214529:0.125562:361194:rs7515915
1   766007  rs61768174  A   C   .   PASS    AF=0.105428 ES:SE:LP:AF:SS:ID   -5.77633e-05:0.000212525:0.104699:0.105428:361194:rs61768174
1   766105  rs2519015   T   A   .   PASS    AF=0.855376 ES:SE:LP:AF:SS:ID   -7.83589e-05:0.000190793:0.166667:0.855376:361194:rs2519015
1   768116  rs376645387 A   AGTTTT  .   PASS    AF=0.838358 ES:SE:LP:AF:SS:ID   -0.000660409:0.000187962:3.35433:0.838358:361194:rs376645387
1   768253  rs2977608   A   C   .   PASS    AF=0.763351 ES:SE:LP:AF:SS:ID   -0.000285765:0.000151276:1.22997:0.763351:361194:rs2977608
1   768448  rs12562034  G   A   .   PASS    AF=0.105921 ES:SE:LP:AF:SS:ID   0.000578862:0.00020842:2.26121:0.105921:361194:rs12562034
1   769138  rs59306077  CAT C   .   PASS    AF=0.128938 ES:SE:LP:AF:SS:ID   -2.78874e-05:0.00019249:0.053151:0.128938:361194:rs762168062
1   769223  rs60320384  C   G   .   PASS    AF=0.128608 ES:SE:LP:AF:SS:ID   -2.5398e-05:0.000192312:0.04821:0.128608:361194:rs60320384
1   769963  rs7518545   G   A   .   PASS    AF=0.105213 ES:SE:LP:AF:SS:ID   0.000574992:0.000209994:2.20907:0.105213:361194:rs7518545
1   770886  rs371458725 G   A   .   PASS    AF=0.104268 ES:SE:LP:AF:SS:ID   0.000593456:0.000211673:2.29645:0.104268:361194:rs371458725
1   771410  rs2519006   C   T   .   PASS    AF=0.830106 ES:SE:LP:AF:SS:ID   7.20392e-06:0.000187386:0.013527:0.830106:361194:rs2519006
1   771823  rs2977605   T   C   .   PASS    AF=0.87012  ES:SE:LP:AF:SS:ID   1.41627e-05:0.000191451:0.0263965:0.87012:361194:rs2977605
1   771967  rs59066358  G   A   .   PASS    AF=0.128668 ES:SE:LP:AF:SS:ID   -2.1735e-05:0.000192223:0.0409715:0.128668:361194:rs59066358