Summary

Summary {data-width=650}

Manhattan plot

manhattan_plot

manhattan_plot

QQ plot

qq_plot

qq_plot

AF plot

af_plot

af_plot

P-Z plot

pz_plot

pz_plot

beta_std plot

beta_std_plot

beta_std_plot

Metadata

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}

LDSC

*********************************************************************
* LD Score Regression (LDSC)
* Version 1.0.1
* (C) 2014-2019 Brendan Bulik-Sullivan and Hilary Finucane
* Broad Institute of MIT and Harvard / MIT Department of Mathematics
* GNU General Public License v3
*********************************************************************
Call: 
./ldsc.py \
--h2 /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/ukb-d-import/processed/ukb-d-41219_3/ukb-d-41219_3.vcf.gz \
--ref-ld-chr /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/ \
--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/ukb-d-import/processed/ukb-d-41219_3/ldsc.txt \
--w-ld-chr /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/ 

Beginning analysis at Mon Nov 25 16:52:43 2019
Reading summary statistics from /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/ukb-d-import/processed/ukb-d-41219_3/ukb-d-41219_3.vcf.gz ...
Read summary statistics for 9673766 SNPs.
Dropped 7279 SNPs with duplicated rs numbers.
Reading reference panel LD Score from /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/[1-22] ...
Read reference panel LD Scores for 1290028 SNPs.
Removing partitioned LD Scores with zero variance.
Reading regression weight LD Score from /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/[1-22] ...
Read regression weight LD Scores for 1290028 SNPs.
After merging with reference panel LD, 1274987 SNPs remain.
After merging with regression SNP LD, 1274987 SNPs remain.
Using two-step estimator with cutoff at 30.
Total Observed scale h2: -0.1034 (0.1252)
Lambda GC: 1.0014
Mean Chi^2: 1.0017
Intercept: 1.0086 (0.0063)
Ratio: 4.9815 (3.6878)
Analysis finished at Mon Nov 25 16:54:15 2019
Total time elapsed: 1.0m:32.91s

QC metrics

Metrics

Metrics

{
    "af_correlation": 0.9398,
    "inflation_factor": 0.9989,
    "mean_EFFECT": 0.0002,
    "n": 3345,
    "n_snps": 9673766,
    "n_clumped_hits": 0,
    "n_p_sig": 0,
    "n_mono": 0,
    "n_ns": 1062112,
    "n_mac": 0,
    "is_snpid_unique": false,
    "n_miss_EFFECT": 0,
    "n_miss_SE": 0,
    "n_miss_PVAL": 0,
    "n_miss_AF": 0,
    "n_miss_AF_reference": 192760,
    "n_est": 3330.7947,
    "ratio_se_n": 0.9979,
    "mean_diff": 0.0002,
    "ratio_diff": 417.8498,
    "sd_y_est1": 0.4355,
    "sd_y_est2": 0.4346,
    "r2_sum1": 0,
    "r2_sum2": 0,
    "r2_sum3": 0,
    "r2_sum4": 0,
    "ldsc_nsnp_merge_refpanel_ld": 1274987,
    "ldsc_nsnp_merge_regression_ld": 1274987,
    "ldsc_observed_scale_h2_beta": "NA",
    "ldsc_observed_scale_h2_se": "NA",
    "ldsc_intercept_beta": 1.0086,
    "ldsc_intercept_se": 0.0063,
    "ldsc_lambda_gc": 1.0014,
    "ldsc_mean_chisq": 1.0017,
    "ldsc_ratio": 5.0588
}

Flags

name value
af_correlation FALSE
inflation_factor FALSE
n TRUE
is_snpid_non_unique TRUE
mean_EFFECT_nonfinite FALSE
mean_EFFECT_05 FALSE
mean_EFFECT_01 FALSE
mean_chisq FALSE
n_p_sig FALSE
miss_EFFECT FALSE
miss_SE FALSE
miss_PVAL FALSE
ldsc_ratio TRUE
ldsc_intercept_beta FALSE
n_clumped_hits FALSE
r2_sum1 FALSE
r2_sum2 FALSE
r2_sum3 FALSE
r2_sum4 FALSE

Definitions

General metrics

  • af_correlation: Correlation coefficient between AF and AF_reference.
  • inflation_factor (lambda): Genomic inflation factor.
  • mean_EFFECT: Mean of EFFECT size.
  • n: Maximum value of reported sample size across all SNPs, \(n\).
  • n_clumped_hits: Number of clumped hits.
  • n_snps: Number of SNPs
  • n_p_sig: Number of SNPs with pvalue below 5e-8.
  • n_mono: Number of monomorphic (MAF == 1 or MAF == 0) SNPs.
  • n_ns: Number of SNPs with nonsense values:
    • alleles other than A, C, G or T.
    • P-values < 0 or > 1.
    • negative or infinite standard errors (<= 0 or = Infinity).
    • infinite beta estimates or allele frequencies < 0 or > 1.
  • n_mac: Number of cases where MAC (\(2 \times N \times MAF\)) is less than 6.
  • is_snpid_unique: true if the combination of ID REF ALT is unique and therefore no duplication in snpid.
  • n_miss_<*>: Number of NA observations for <*> column.

se_n metrics

  • n_est: Estimated sample size value, \(\widehat{n}\).
  • ratio_se_n: \(\texttt{ratio_se_n} = \frac{\sqrt{\widehat{n}}}{\sqrt{n}}\). We expect ratio_se_n to be 1. When it is not 1, it implies that the trait did not have a variance of 1, the reported sample size is wrong, or that the SNP-level effective sample sizes differ markedly from the reported sample size.
  • mean_diff: \(\texttt{mean_diff} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta_j}{\texttt{n_snps}}\), mean difference between the standardised beta, predicted from P-values, and the observed beta. The difference should be very close to zero if trait has a variance of 1.
    • \(\widehat{\beta_j^{std}} = \sqrt{\frac{{z}_j^2 / ({z}_j^2 + n -2)}{2 \times {MAF}_j \times (1 - {MAF}_j)}} \times sign({z}_j)\),
    • \({z}_j = \frac{\beta_j}{{se}_j}\),
    • and \(\beta_j\) is the reported effect size.
  • ratio_diff: \(\texttt{ratio_diff} = |\frac{\texttt{mean_diff}}{\texttt{mean_diff2}}|\), absolute ratio between the mean of diff and the mean of diff2 (expected difference between the standardised beta predicted from P-values, and the standardised beta derived from the observed beta divided by the predicted SD; NOT reported). The ratio should be close to 1. If different from 1, then implies that the betas are not in a standard deviation scale.
    • \(\texttt{mean_diff2} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta^{\prime}_j}{\texttt{n_snps}}\)
    • \(\beta^{\prime}_j = \frac{\beta_j}{\widehat{\texttt{sd2}}_{y}}\)
  • sd_y_est1: The standard deviation for the trait inferred from the reported sample size, median standard errors for the SNP-trait assocations and SNP variances.
    • \(\widehat{\texttt{sd1}}_{y} = \frac{\sqrt{n} \times median({se}_j)}{C}\),
    • \(C = median(\frac{1}{\sqrt{2 \times {MAF}_j \times (1 - {MAF}_j)}})\),
    • and \({se}_j\) is the reported standard error.
  • sd_y_est2: The standard deviation for the trait inferred from the reported sample size, Z statistics for the SNP-trait effects (beta/se) and allele frequency.
    • \(\widehat{\texttt{sd2}}_{y} = median(\widehat{sd_j})\),
    • \(\widehat{sd_j} = \frac{\beta_j}{\widehat{\beta_j^{std}}}\),

r2 metrics

Sum of variance explained, calculated from the clumped top hits sample.

  • r2_sum<*>: r2 statistics under various assumptions
    • 1: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var1}}}\), \(\texttt{var1} = 1\).
    • 2: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var2}}}\), \(\texttt{var2} = {\widehat{\texttt{sd1}}_{y}}^2\),
    • 3: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var3}}}\), \(\texttt{var3} = {\widehat{\texttt{sd2}}_{y}}^2\),
    • 4: \(r^2 = \sum_j{\frac{F_j}{F_j + n - 2}}\), \(F = \frac{\beta_j^2}{{se}_j^2}\).

LDSC metrics

Metrics from LD regression

  • ldsc_nsnp_merge_refpanel_ld: Number of remaining SNPs after merging with reference panel LD.
  • ldsc_nsnp_merge_regression_ld: Number of remaining SNPs after merging with regression SNP LD.
  • ldsc_observed_scale_h2_{beta,se} Coefficient value and SE for total observed scale h2.
  • ldsc_intercept_{beta,se}: Coefficient value and SE for intercept. Intercept is expected to be 1.
  • ldsc_lambda_gc: Lambda GC statistics.
  • ldsc_mean_chisq: Mean \(\chi^2\) statistics.
  • ldsc_ratio: \(\frac{\texttt{ldsc_intercept_beta} - 1}{\texttt{ldsc_mean_chisq} - 1}\), the proportion of the inflation in the mean \(\chi^2\) that the LD Score regression intercepts ascribes to causes other than polygenic heritability. The value of ratio should be close to zero, though in practice values of 0.1-0.2 are not uncommon, probably due to sample/reference LD Score mismatch or model misspecification (e.g., low LD variants have slightly higher \(h^2\) per SNP).

Flags

When a metric needs attention, the flag should return TRUE.

  • af_correlation: abs(af_correlation) < 0.7.
  • inflation_factor: inflation_factor > 1.2.
  • n: n (max reported sample size) < 10000.
  • is_snpid_non_unique: NOT is_snpid_unique.
  • mean_EFFECT_nonfinite: mean(EFFECT) is NA, NaN, or Inf.
  • mean_EFFECT_05: abs(mean(EFFECT)) > 0.5.
  • mean_EFFECT_01: abs(mean(EFFECT)) > 0.1.
  • mean_chisq: ldsc_mean_chisq > 1.3 or ldsc_mean_chisq < 0.7.
  • n_p_sig: n_p_sig > 1000.
  • miss_<*>: n_miss_<*> / n_snps > 0.01.
  • ldsc_ratio: ldsc_ratio > 0.5
  • ldsc_intercept_beta: ldsc_intercept_beta > 1.5
  • n_clumped_hits: n_clumped_hits > 1000
  • r2_sum<*>: r2_sum<*> > 0.5

Plots

  • Manhattan plot
    • Red line: \(-log_{10}^{5 \times 10^{-8}}\)
    • Blue line: \(-log_{10}^{5 \times 10^{-5}}\)
  • QQ plot
  • AF plot
  • P-Z plot
  • beta_std plot: Scatter plot between \(\widehat{\beta_j^{std}}\) and \(\beta_j\)

Diagnostics

Details

Summary stats

skim_type skim_variable n_missing complete_rate character.min character.max character.empty character.n_unique character.whitespace numeric.mean numeric.sd numeric.p0 numeric.p25 numeric.p50 numeric.p75 numeric.p100 numeric.hist
character ID 0 1.0000000 3 94 0 9667037 0 NA NA NA NA NA NA NA NA
character REF 0 1.0000000 1 98 0 47018 0 NA NA NA NA NA NA NA NA
character ALT 0 1.0000000 1 342 0 29687 0 NA NA NA NA NA NA NA NA
numeric CHROM 0 1.0000000 NA NA NA NA NA 9.079201e+00 6.175339e+00 1.0000000 4.000000e+00 8.000000e+00 1.300000e+01 2.300000e+01 ▇▅▅▂▂
numeric POS 0 1.0000000 NA NA NA NA NA 7.872808e+07 5.611156e+07 302.0000000 3.242804e+07 6.960162e+07 1.147247e+08 2.492297e+08 ▇▆▅▂▁
numeric EFFECT 0 1.0000000 NA NA NA NA NA 1.586000e-04 2.390200e-02 -0.2099920 -1.139130e-02 3.480000e-05 1.149930e-02 2.517130e-01 ▁▁▇▁▁
numeric SE 0 1.0000000 NA NA NA NA NA 2.077180e-02 1.179010e-02 0.0102526 1.165300e-02 1.553280e-02 2.657100e-02 6.781530e-02 ▇▂▁▁▁
numeric PVAL 0 1.0000000 NA NA NA NA NA 4.998545e-01 2.886051e-01 0.0000003 2.499539e-01 5.002384e-01 7.497613e-01 1.000000e+00 ▇▇▇▇▇
numeric PVAL_ztest 0 1.0000000 NA NA NA NA NA 4.998065e-01 2.886340e-01 0.0000003 2.498708e-01 5.001913e-01 7.497414e-01 1.000000e+00 ▇▇▇▇▇
numeric AF 0 1.0000000 NA NA NA NA NA 2.622775e-01 2.628735e-01 0.0099686 4.458340e-02 1.603860e-01 4.173290e-01 9.900310e-01 ▇▂▂▁▁
numeric AF_reference 192760 0.9800739 NA NA NA NA NA 2.617310e-01 2.536863e-01 0.0000000 5.071880e-02 1.755190e-01 4.127400e-01 1.000000e+00 ▇▃▂▁▁
numeric N 0 1.0000000 NA NA NA NA NA 3.345000e+03 0.000000e+00 3345.0000000 3.345000e+03 3.345000e+03 3.345000e+03 3.345000e+03 ▁▁▇▁▁

Head and tail

CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
1 692794 rs530212009 CA C 0.0315765 0.0184339 0.0868140 0.0867202 0.1124020 0.1894970 3345
1 693731 rs12238997 A G 0.0355537 0.0174870 0.0421163 0.0420373 0.1170260 0.1417730 3345
1 707522 rs371890604 G C 0.0387865 0.0198513 0.0508031 0.0507187 0.0975873 0.1293930 3345
1 717587 rs144155419 G A 0.0005243 0.0455998 0.9908260 0.9908257 0.0168393 0.0045926 3345
1 730087 rs148120343 T C 0.0544886 0.0241000 0.0238276 0.0237631 0.0585521 0.0127796 3345
1 731718 rs142557973 T C 0.0364195 0.0165944 0.0282553 0.0281861 0.1229470 0.1543530 3345
1 732032 rs61770163 A C 0.0319719 0.0177296 0.0714315 0.0713401 0.1228790 0.1555510 3345
1 734349 rs141242758 T C 0.0375004 0.0165808 0.0237821 0.0237174 0.1220140 0.1525560 3345
1 749963 rs529266287 T TAA -0.0275784 0.0161719 0.0882287 0.0881332 0.8685380 0.7641770 3345
1 751343 rs28544273 T A 0.0269185 0.0161054 0.0947393 0.0946435 0.1242360 0.2426120 3345
CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
23 154923311 rs141127553 C T 0.0216898 0.0232270 0.3504661 0.3503976 0.0569700 0.0309934 3345
23 154923374 rs111332691 T A -0.0200428 0.0251998 0.4264626 0.4264067 0.0457399 0.0116556 3345
23 154925045 rs509981 C T 0.0054424 0.0119441 0.6486658 0.6486368 0.2521760 0.3634440 3345
23 154925895 rs538470 C T 0.0078066 0.0122301 0.5233124 0.5232695 0.2482110 0.3634440 3345
23 154927581 rs644138 G A 0.0102305 0.0113469 0.3673314 0.3672631 0.3095370 0.4635760 3345
23 154929412 rs557132 C T 0.0056095 0.0119451 0.6386663 0.6386366 0.2520330 0.3568210 3345
23 154929637 rs35185538 CT C 0.0087655 0.0125826 0.4860792 0.4860302 0.2348110 0.3011920 3345
23 154929952 rs4012982 CAA C 0.0046189 0.0126187 0.7143614 0.7143387 0.2454930 0.3165560 3345
23 154930230 rs781880 A G 0.0063084 0.0119518 0.5976584 0.5976225 0.2519210 0.3618540 3345
23 154930487 rs781879 T A 0.0279522 0.0395475 0.4797401 0.4796904 0.0218471 0.1263580 3345

bcf preview

1   692794  rs530212009 CA  C   .   PASS    AF=0.112402 ES:SE:LP:AF:SS:ID   0.0315765:0.0184339:1.06141:0.112402:3345:1_692794_CA_C
1   693731  rs12238997  A   G   .   PASS    AF=0.117026 ES:SE:LP:AF:SS:ID   0.0355537:0.017487:1.37555:0.117026:3345:rs12238997
1   707522  rs371890604 G   C   .   PASS    AF=0.0975873    ES:SE:LP:AF:SS:ID   0.0387865:0.0198513:1.29411:0.0975873:3345:rs371890604
1   717587  rs144155419 G   A   .   PASS    AF=0.0168393    ES:SE:LP:AF:SS:ID   0.000524333:0.0455998:0.00400261:0.0168393:3345:rs144155419
1   730087  rs148120343 T   C   .   PASS    AF=0.0585521    ES:SE:LP:AF:SS:ID   0.0544886:0.0241:1.62292:0.0585521:3345:rs148120343
1   731718  rs58276399  T   C   .   PASS    AF=0.122947 ES:SE:LP:AF:SS:ID   0.0364195:0.0165944:1.5489:0.122947:3345:rs58276399
1   732032  rs61770163  A   C   .   PASS    AF=0.122879 ES:SE:LP:AF:SS:ID   0.0319719:0.0177296:1.14611:0.122879:3345:rs61770163
1   734349  rs141242758 T   C   .   PASS    AF=0.122014 ES:SE:LP:AF:SS:ID   0.0375004:0.0165808:1.62375:0.122014:3345:rs141242758
1   749963  rs529266287 T   TAA .   PASS    AF=0.868538 ES:SE:LP:AF:SS:ID   -0.0275784:0.0161719:1.05439:0.868538:3345:rs529266287
1   751343  rs28544273  T   A   .   PASS    AF=0.124236 ES:SE:LP:AF:SS:ID   0.0269185:0.0161054:1.02347:0.124236:3345:rs28544273
1   751488  rs200141114 G   GA  .   PASS    AF=0.141999 ES:SE:LP:AF:SS:ID   0.0208667:0.0161143:0.708991:0.141999:3345:rs200141114
1   751756  rs28527770  T   C   .   PASS    AF=0.124292 ES:SE:LP:AF:SS:ID   0.0268461:0.016091:1.02076:0.124292:3345:rs28527770
1   753405  rs3115860   C   A   .   PASS    AF=0.869637 ES:SE:LP:AF:SS:ID   -0.0269561:0.0156767:1.06744:0.869637:3345:rs3115860
1   753425  rs3131970   T   C   .   PASS    AF=0.873655 ES:SE:LP:AF:SS:ID   -0.0249154:0.0159128:0.929947:0.873655:3345:rs3131970
1   753541  rs2073813   G   A   .   PASS    AF=0.129651 ES:SE:LP:AF:SS:ID   0.0297101:0.0157414:1.22771:0.129651:3345:rs2073813
1   754105  rs12184325  C   T   .   PASS    AF=0.0415546    ES:SE:LP:AF:SS:ID   0.00169174:0.0270337:0.0222279:0.0415546:3345:rs12184325
1   754182  rs3131969   A   G   .   PASS    AF=0.869262 ES:SE:LP:AF:SS:ID   -0.027364:0.0156563:1.09371:0.869262:3345:rs3131969
1   754192  rs3131968   A   G   .   PASS    AF=0.869262 ES:SE:LP:AF:SS:ID   -0.0273225:0.0156555:1.09132:0.869262:3345:rs3131968
1   754334  rs3131967   T   C   .   PASS    AF=0.869256 ES:SE:LP:AF:SS:ID   -0.0273447:0.0156548:1.09271:0.869256:3345:rs3131967
1   755890  rs3115858   A   T   .   PASS    AF=0.868938 ES:SE:LP:AF:SS:ID   -0.0282427:0.0156176:1.15097:0.868938:3345:rs3115858
1   756434  rs61768170  G   C   .   PASS    AF=0.126814 ES:SE:LP:AF:SS:ID   0.0296017:0.0159946:1.19181:0.126814:3345:rs61768170
1   756604  rs3131962   A   G   .   PASS    AF=0.868759 ES:SE:LP:AF:SS:ID   -0.0284479:0.0155886:1.16683:0.868759:3345:rs3131962
1   757640  rs3115853   G   A   .   PASS    AF=0.867793 ES:SE:LP:AF:SS:ID   -0.0274138:0.0155703:1.10573:0.867793:3345:rs3115853
1   757734  rs4951929   C   T   .   PASS    AF=0.868643 ES:SE:LP:AF:SS:ID   -0.0282095:0.0155925:1.15172:0.868643:3345:rs4951929
1   757936  rs4951862   C   A   .   PASS    AF=0.868646 ES:SE:LP:AF:SS:ID   -0.0282117:0.0155935:1.15174:0.868646:3345:rs4951862
1   758144  rs3131956   A   G   .   PASS    AF=0.868658 ES:SE:LP:AF:SS:ID   -0.0282263:0.0155946:1.15251:0.868658:3345:rs3131956
1   758626  rs3131954   C   T   .   PASS    AF=0.869132 ES:SE:LP:AF:SS:ID   -0.0274875:0.0156338:1.10345:0.869132:3345:rs3131954
1   759293  rs10157329  T   A   .   PASS    AF=0.0979091    ES:SE:LP:AF:SS:ID   0.0362748:0.0185542:1.29535:0.0979091:3345:rs10157329
1   759837  rs3115851   T   A   .   PASS    AF=0.873294 ES:SE:LP:AF:SS:ID   -0.0249551:0.0159011:0.933108:0.873294:3345:rs3115851
1   761732  rs2286139   C   T   .   PASS    AF=0.862376 ES:SE:LP:AF:SS:ID   -0.027336:0.0155934:1.09862:0.862376:3345:rs2286139
1   761752  rs1057213   C   T   .   PASS    AF=0.868107 ES:SE:LP:AF:SS:ID   -0.0267514:0.0157406:1.04907:0.868107:3345:rs1057213
1   762273  rs3115849   G   A   .   PASS    AF=0.865048 ES:SE:LP:AF:SS:ID   -0.0289672:0.015767:1.17869:0.865048:3345:rs3115849
1   762485  rs12095200  C   A   .   PASS    AF=0.100185 ES:SE:LP:AF:SS:ID   0.0183092:0.0187921:0.481518:0.100185:3345:rs12095200
1   762589  rs3115848   G   C   .   PASS    AF=0.870606 ES:SE:LP:AF:SS:ID   -0.0269124:0.0159659:1.03639:0.870606:3345:rs3115848
1   762592  rs3131950   C   G   .   PASS    AF=0.870606 ES:SE:LP:AF:SS:ID   -0.0269111:0.0159658:1.03632:0.870606:3345:rs3131950
1   762601  rs3131949   T   C   .   PASS    AF=0.870601 ES:SE:LP:AF:SS:ID   -0.0269105:0.0159658:1.03628:0.870601:3345:rs3131949
1   762632  rs3131948   T   A   .   PASS    AF=0.870733 ES:SE:LP:AF:SS:ID   -0.0267084:0.0159646:1.02491:0.870733:3345:rs3131948
1   764191  rs7515915   T   G   .   PASS    AF=0.126593 ES:SE:LP:AF:SS:ID   0.0281901:0.0159759:1.1094:0.126593:3345:rs7515915
1   766007  rs61768174  A   C   .   PASS    AF=0.10536  ES:SE:LP:AF:SS:ID   0.0383336:0.0174638:1.54927:0.10536:3345:rs61768174
1   766105  rs2519015   T   A   .   PASS    AF=0.853371 ES:SE:LP:AF:SS:ID   -0.0273432:0.0154902:1.11:0.853371:3345:rs2519015
1   768116  rs376645387 A   AGTTTT  .   PASS    AF=0.833954 ES:SE:LP:AF:SS:ID   0.00538659:0.0153028:0.139746:0.833954:3345:rs376645387
1   768253  rs2977608   A   C   .   PASS    AF=0.760547 ES:SE:LP:AF:SS:ID   -0.0106334:0.0123502:0.409708:0.760547:3345:rs2977608
1   768448  rs12562034  G   A   .   PASS    AF=0.107623 ES:SE:LP:AF:SS:ID   -0.0138238:0.0169693:0.381595:0.107623:3345:rs12562034
1   769138  rs59306077  CAT C   .   PASS    AF=0.130185 ES:SE:LP:AF:SS:ID   0.0305494:0.0157262:1.28273:0.130185:3345:rs762168062
1   769223  rs60320384  C   G   .   PASS    AF=0.1298   ES:SE:LP:AF:SS:ID   0.0312928:0.0157138:1.3324:0.1298:3345:rs60320384
1   769963  rs7518545   G   A   .   PASS    AF=0.10685  ES:SE:LP:AF:SS:ID   -0.0130917:0.0171095:0.3524:0.10685:3345:rs7518545
1   770886  rs371458725 G   A   .   PASS    AF=0.105814 ES:SE:LP:AF:SS:ID   -0.0135032:0.0172576:0.362505:0.105814:3345:rs371458725
1   771410  rs2519006   C   T   .   PASS    AF=0.82751  ES:SE:LP:AF:SS:ID   -0.0237877:0.0152451:0.925286:0.82751:3345:rs2519006
1   771823  rs2977605   T   C   .   PASS    AF=0.868744 ES:SE:LP:AF:SS:ID   -0.028717:0.0156148:1.18051:0.868744:3345:rs2977605
1   771967  rs59066358  G   A   .   PASS    AF=0.12988  ES:SE:LP:AF:SS:ID   0.030898:0.0157022:1.30821:0.12988:3345:rs59066358