Summary

Summary {data-width=650}

Manhattan plot

manhattan_plot

manhattan_plot

QQ plot

qq_plot

qq_plot

AF plot

af_plot

af_plot

P-Z plot

pz_plot

pz_plot

beta_std plot

beta_std_plot

beta_std_plot

Metadata

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}

LDSC

*********************************************************************
* LD Score Regression (LDSC)
* Version 1.0.1
* (C) 2014-2019 Brendan Bulik-Sullivan and Hilary Finucane
* Broad Institute of MIT and Harvard / MIT Department of Mathematics
* GNU General Public License v3
*********************************************************************
Call: 
./ldsc.py \
--h2 /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/ukb-d-import/processed/ukb-d-22601_22113066/ukb-d-22601_22113066.vcf.gz \
--ref-ld-chr /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/ \
--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/ukb-d-import/processed/ukb-d-22601_22113066/ldsc.txt \
--w-ld-chr /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/ 

Beginning analysis at Mon Nov 25 14:45:08 2019
Reading summary statistics from /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/ukb-d-import/processed/ukb-d-22601_22113066/ukb-d-22601_22113066.vcf.gz ...
Read summary statistics for 9930734 SNPs.
Dropped 7637 SNPs with duplicated rs numbers.
Reading reference panel LD Score from /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/[1-22] ...
Read reference panel LD Scores for 1290028 SNPs.
Removing partitioned LD Scores with zero variance.
Reading regression weight LD Score from /mnt/storage/private/mrcieu/research/scratch/IGD/data/dev/reference/eur_w_ld_chr/[1-22] ...
Read regression weight LD Scores for 1290028 SNPs.
After merging with reference panel LD, 1277581 SNPs remain.
After merging with regression SNP LD, 1277581 SNPs remain.
Using two-step estimator with cutoff at 30.
Total Observed scale h2: 0.0278 (0.0051)
Lambda GC: 1.0442
Mean Chi^2: 1.0436
Intercept: 0.9946 (0.0059)
Ratio < 0 (usually indicates GC correction).
Analysis finished at Mon Nov 25 14:46:48 2019
Total time elapsed: 1.0m:40.85s

QC metrics

Metrics

Metrics

{
    "af_correlation": 0.9413,
    "inflation_factor": 1.042,
    "mean_EFFECT": -0,
    "n": 89866,
    "n_snps": 9930734,
    "n_clumped_hits": 0,
    "n_p_sig": 1,
    "n_mono": 0,
    "n_ns": 1078531,
    "n_mac": 0,
    "is_snpid_unique": false,
    "n_miss_EFFECT": 0,
    "n_miss_SE": 0,
    "n_miss_PVAL": 0,
    "n_miss_AF": 0,
    "n_miss_AF_reference": 196595,
    "n_est": 89880.889,
    "ratio_se_n": 1.0001,
    "mean_diff": -0.0001,
    "ratio_diff": 85.4412,
    "sd_y_est1": 0.1271,
    "sd_y_est2": 0.1271,
    "r2_sum1": 0,
    "r2_sum2": 0,
    "r2_sum3": 0,
    "r2_sum4": 0,
    "ldsc_nsnp_merge_refpanel_ld": 1277581,
    "ldsc_nsnp_merge_regression_ld": 1277581,
    "ldsc_observed_scale_h2_beta": 0.0278,
    "ldsc_observed_scale_h2_se": 0.0051,
    "ldsc_intercept_beta": 0.9946,
    "ldsc_intercept_se": 0.0059,
    "ldsc_lambda_gc": 1.0442,
    "ldsc_mean_chisq": 1.0436,
    "ldsc_ratio": -0.1239
}

Flags

name value
af_correlation FALSE
inflation_factor FALSE
n FALSE
is_snpid_non_unique TRUE
mean_EFFECT_nonfinite FALSE
mean_EFFECT_05 FALSE
mean_EFFECT_01 FALSE
mean_chisq FALSE
n_p_sig FALSE
miss_EFFECT FALSE
miss_SE FALSE
miss_PVAL FALSE
ldsc_ratio FALSE
ldsc_intercept_beta FALSE
n_clumped_hits FALSE
r2_sum1 FALSE
r2_sum2 FALSE
r2_sum3 FALSE
r2_sum4 FALSE

Definitions

General metrics

  • af_correlation: Correlation coefficient between AF and AF_reference.
  • inflation_factor (lambda): Genomic inflation factor.
  • mean_EFFECT: Mean of EFFECT size.
  • n: Maximum value of reported sample size across all SNPs, \(n\).
  • n_clumped_hits: Number of clumped hits.
  • n_snps: Number of SNPs
  • n_p_sig: Number of SNPs with pvalue below 5e-8.
  • n_mono: Number of monomorphic (MAF == 1 or MAF == 0) SNPs.
  • n_ns: Number of SNPs with nonsense values:
    • alleles other than A, C, G or T.
    • P-values < 0 or > 1.
    • negative or infinite standard errors (<= 0 or = Infinity).
    • infinite beta estimates or allele frequencies < 0 or > 1.
  • n_mac: Number of cases where MAC (\(2 \times N \times MAF\)) is less than 6.
  • is_snpid_unique: true if the combination of ID REF ALT is unique and therefore no duplication in snpid.
  • n_miss_<*>: Number of NA observations for <*> column.

se_n metrics

  • n_est: Estimated sample size value, \(\widehat{n}\).
  • ratio_se_n: \(\texttt{ratio_se_n} = \frac{\sqrt{\widehat{n}}}{\sqrt{n}}\). We expect ratio_se_n to be 1. When it is not 1, it implies that the trait did not have a variance of 1, the reported sample size is wrong, or that the SNP-level effective sample sizes differ markedly from the reported sample size.
  • mean_diff: \(\texttt{mean_diff} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta_j}{\texttt{n_snps}}\), mean difference between the standardised beta, predicted from P-values, and the observed beta. The difference should be very close to zero if trait has a variance of 1.
    • \(\widehat{\beta_j^{std}} = \sqrt{\frac{{z}_j^2 / ({z}_j^2 + n -2)}{2 \times {MAF}_j \times (1 - {MAF}_j)}} \times sign({z}_j)\),
    • \({z}_j = \frac{\beta_j}{{se}_j}\),
    • and \(\beta_j\) is the reported effect size.
  • ratio_diff: \(\texttt{ratio_diff} = |\frac{\texttt{mean_diff}}{\texttt{mean_diff2}}|\), absolute ratio between the mean of diff and the mean of diff2 (expected difference between the standardised beta predicted from P-values, and the standardised beta derived from the observed beta divided by the predicted SD; NOT reported). The ratio should be close to 1. If different from 1, then implies that the betas are not in a standard deviation scale.
    • \(\texttt{mean_diff2} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta^{\prime}_j}{\texttt{n_snps}}\)
    • \(\beta^{\prime}_j = \frac{\beta_j}{\widehat{\texttt{sd2}}_{y}}\)
  • sd_y_est1: The standard deviation for the trait inferred from the reported sample size, median standard errors for the SNP-trait assocations and SNP variances.
    • \(\widehat{\texttt{sd1}}_{y} = \frac{\sqrt{n} \times median({se}_j)}{C}\),
    • \(C = median(\frac{1}{\sqrt{2 \times {MAF}_j \times (1 - {MAF}_j)}})\),
    • and \({se}_j\) is the reported standard error.
  • sd_y_est2: The standard deviation for the trait inferred from the reported sample size, Z statistics for the SNP-trait effects (beta/se) and allele frequency.
    • \(\widehat{\texttt{sd2}}_{y} = median(\widehat{sd_j})\),
    • \(\widehat{sd_j} = \frac{\beta_j}{\widehat{\beta_j^{std}}}\),

r2 metrics

Sum of variance explained, calculated from the clumped top hits sample.

  • r2_sum<*>: r2 statistics under various assumptions
    • 1: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var1}}}\), \(\texttt{var1} = 1\).
    • 2: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var2}}}\), \(\texttt{var2} = {\widehat{\texttt{sd1}}_{y}}^2\),
    • 3: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var3}}}\), \(\texttt{var3} = {\widehat{\texttt{sd2}}_{y}}^2\),
    • 4: \(r^2 = \sum_j{\frac{F_j}{F_j + n - 2}}\), \(F = \frac{\beta_j^2}{{se}_j^2}\).

LDSC metrics

Metrics from LD regression

  • ldsc_nsnp_merge_refpanel_ld: Number of remaining SNPs after merging with reference panel LD.
  • ldsc_nsnp_merge_regression_ld: Number of remaining SNPs after merging with regression SNP LD.
  • ldsc_observed_scale_h2_{beta,se} Coefficient value and SE for total observed scale h2.
  • ldsc_intercept_{beta,se}: Coefficient value and SE for intercept. Intercept is expected to be 1.
  • ldsc_lambda_gc: Lambda GC statistics.
  • ldsc_mean_chisq: Mean \(\chi^2\) statistics.
  • ldsc_ratio: \(\frac{\texttt{ldsc_intercept_beta} - 1}{\texttt{ldsc_mean_chisq} - 1}\), the proportion of the inflation in the mean \(\chi^2\) that the LD Score regression intercepts ascribes to causes other than polygenic heritability. The value of ratio should be close to zero, though in practice values of 0.1-0.2 are not uncommon, probably due to sample/reference LD Score mismatch or model misspecification (e.g., low LD variants have slightly higher \(h^2\) per SNP).

Flags

When a metric needs attention, the flag should return TRUE.

  • af_correlation: abs(af_correlation) < 0.7.
  • inflation_factor: inflation_factor > 1.2.
  • n: n (max reported sample size) < 10000.
  • is_snpid_non_unique: NOT is_snpid_unique.
  • mean_EFFECT_nonfinite: mean(EFFECT) is NA, NaN, or Inf.
  • mean_EFFECT_05: abs(mean(EFFECT)) > 0.5.
  • mean_EFFECT_01: abs(mean(EFFECT)) > 0.1.
  • mean_chisq: ldsc_mean_chisq > 1.3 or ldsc_mean_chisq < 0.7.
  • n_p_sig: n_p_sig > 1000.
  • miss_<*>: n_miss_<*> / n_snps > 0.01.
  • ldsc_ratio: ldsc_ratio > 0.5
  • ldsc_intercept_beta: ldsc_intercept_beta > 1.5
  • n_clumped_hits: n_clumped_hits > 1000
  • r2_sum<*>: r2_sum<*> > 0.5

Plots

  • Manhattan plot
    • Red line: \(-log_{10}^{5 \times 10^{-8}}\)
    • Blue line: \(-log_{10}^{5 \times 10^{-5}}\)
  • QQ plot
  • AF plot
  • P-Z plot
  • beta_std plot: Scatter plot between \(\widehat{\beta_j^{std}}\) and \(\beta_j\)

Diagnostics

Details

Summary stats

skim_type skim_variable n_missing complete_rate character.min character.max character.empty character.n_unique character.whitespace numeric.mean numeric.sd numeric.p0 numeric.p25 numeric.p50 numeric.p75 numeric.p100 numeric.hist
character ID 0 1.0000000 3 94 0 9923647 0 NA NA NA NA NA NA NA NA
character REF 0 1.0000000 1 98 0 47952 0 NA NA NA NA NA NA NA NA
character ALT 0 1.0000000 1 342 0 30036 0 NA NA NA NA NA NA NA NA
numeric CHROM 0 1.0000000 NA NA NA NA NA 9.080643e+00 6.177095e+00 1.00000e+00 4.000000e+00 8.000000e+00 1.300000e+01 2.300000e+01 ▇▅▅▂▂
numeric POS 0 1.0000000 NA NA NA NA NA 7.875464e+07 5.609486e+07 3.02000e+02 3.246510e+07 6.965364e+07 1.147374e+08 2.492297e+08 ▇▆▅▂▁
numeric EFFECT 0 1.0000000 NA NA NA NA NA -1.120000e-05 1.436200e-03 -1.63593e-02 -6.912000e-04 -1.680000e-05 6.537000e-04 1.842560e-02 ▁▁▇▁▁
numeric SE 0 1.0000000 NA NA NA NA NA 1.221600e-03 7.320000e-04 4.95600e-04 6.593000e-04 8.935000e-04 1.577200e-03 4.070400e-03 ▇▂▁▁▁
numeric PVAL 0 1.0000000 NA NA NA NA NA 4.936342e-01 2.901723e-01 0.00000e+00 2.409412e-01 4.911306e-01 7.446154e-01 1.000000e+00 ▇▇▇▇▇
numeric PVAL_ztest 0 1.0000000 NA NA NA NA NA 4.936324e-01 2.901734e-01 0.00000e+00 2.409384e-01 4.911286e-01 7.446138e-01 1.000000e+00 ▇▇▇▇▇
numeric AF 0 1.0000000 NA NA NA NA NA 2.561496e-01 2.629461e-01 8.52080e-03 3.968250e-02 1.516520e-01 4.084190e-01 9.914790e-01 ▇▂▂▁▁
numeric AF_reference 196595 0.9802034 NA NA NA NA NA 2.557703e-01 2.537724e-01 0.00000e+00 4.432910e-02 1.675320e-01 4.045530e-01 1.000000e+00 ▇▃▂▁▁
numeric N 0 1.0000000 NA NA NA NA NA 8.986600e+04 0.000000e+00 8.98660e+04 8.986600e+04 8.986600e+04 8.986600e+04 8.986600e+04 ▁▁▇▁▁

Head and tail

CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
1 692794 rs530212009 CA C 0.0003013 0.0010471 0.7735297 0.7735278 0.1114870 0.1894970 89866
1 693731 rs12238997 A G 0.0006387 0.0009919 0.5196285 0.5196260 0.1164980 0.1417730 89866
1 707522 rs371890604 G C 0.0002899 0.0011151 0.7949049 0.7949050 0.0978285 0.1293930 89866
1 717587 rs144155419 G A -0.0010390 0.0026561 0.6956776 0.6956756 0.0158090 0.0045926 89866
1 730087 rs148120343 T C -0.0004545 0.0013754 0.7410457 0.7410447 0.0569661 0.0127796 89866
1 731718 rs142557973 T C 0.0001878 0.0009413 0.8418360 0.8418354 0.1223780 0.1543530 89866
1 732032 rs61770163 A C 0.0002264 0.0010035 0.8215381 0.8215376 0.1220530 0.1555510 89866
1 734349 rs141242758 T C 0.0002440 0.0009420 0.7955880 0.7955866 0.1216170 0.1525560 89866
1 749963 rs529266287 T TAA -0.0004073 0.0009267 0.6602676 0.6602659 0.8689590 0.7641770 89866
1 751343 rs28544273 T A 0.0002736 0.0009187 0.7658346 0.7658333 0.1236780 0.2426120 89866
CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
23 154923311 rs141127553 C T -0.0009340 0.0011017 0.3965793 0.3965775 0.0553576 0.0309934 89866
23 154923374 rs111332691 T A 0.0003617 0.0011994 0.7630114 0.7630117 0.0449113 0.0116556 89866
23 154925045 rs509981 C T -0.0000347 0.0005784 0.9522051 0.9522047 0.2444280 0.3634440 89866
23 154925895 rs538470 C T 0.0000085 0.0005915 0.9884810 0.9884810 0.2408760 0.3634440 89866
23 154927581 rs644138 G A -0.0002727 0.0005441 0.6162827 0.6162820 0.3001910 0.4635760 89866
23 154929412 rs557132 C T -0.0000269 0.0005786 0.9628790 0.9628793 0.2442750 0.3568210 89866
23 154929637 rs35185538 CT C 0.0001142 0.0006042 0.8500260 0.8500256 0.2286930 0.3011920 89866
23 154929952 rs4012982 CAA C -0.0001894 0.0006089 0.7558037 0.7558019 0.2381980 0.3165560 89866
23 154930230 rs781880 A G -0.0000325 0.0005784 0.9551971 0.9551968 0.2447120 0.3618540 89866
23 154930487 rs781879 T A -0.0027388 0.0019622 0.1627830 0.1627798 0.0196791 0.1263580 89866

bcf preview

1   692794  rs530212009 CA  C   .   PASS    AF=0.111487 ES:SE:LP:AF:SS:ID   0.00030132:0.00104711:0.111523:0.111487:89866:1_692794_CA_C
1   693731  rs12238997  A   G   .   PASS    AF=0.116498 ES:SE:LP:AF:SS:ID   0.000638703:0.000991895:0.284307:0.116498:89866:rs12238997
1   707522  rs371890604 G   C   .   PASS    AF=0.0978285    ES:SE:LP:AF:SS:ID   0.000289869:0.00111511:0.0996848:0.0978285:89866:rs371890604
1   717587  rs144155419 G   A   .   PASS    AF=0.015809 ES:SE:LP:AF:SS:ID   -0.00103898:0.00265612:0.157592:0.015809:89866:rs144155419
1   730087  rs148120343 T   C   .   PASS    AF=0.0569661    ES:SE:LP:AF:SS:ID   -0.000454532:0.00137541:0.130155:0.0569661:89866:rs148120343
1   731718  rs58276399  T   C   .   PASS    AF=0.122378 ES:SE:LP:AF:SS:ID   0.000187825:0.00094126:0.0747725:0.122378:89866:rs58276399
1   732032  rs61770163  A   C   .   PASS    AF=0.122053 ES:SE:LP:AF:SS:ID   0.000226355:0.00100349:0.0853723:0.122053:89866:rs61770163
1   734349  rs141242758 T   C   .   PASS    AF=0.121617 ES:SE:LP:AF:SS:ID   0.000244027:0.00094196:0.0993118:0.121617:89866:rs141242758
1   749963  rs529266287 T   TAA .   PASS    AF=0.868959 ES:SE:LP:AF:SS:ID   -0.000407348:0.000926747:0.18028:0.868959:89866:rs529266287
1   751343  rs28544273  T   A   .   PASS    AF=0.123678 ES:SE:LP:AF:SS:ID   0.000273627:0.000918737:0.115865:0.123678:89866:rs28544273
1   751488  rs200141114 G   GA  .   PASS    AF=0.143191 ES:SE:LP:AF:SS:ID   0.000592713:0.0009096:0.28849:0.143191:89866:rs200141114
1   751756  rs28527770  T   C   .   PASS    AF=0.123813 ES:SE:LP:AF:SS:ID   0.000353544:0.000917412:0.154925:0.123813:89866:rs28527770
1   753405  rs3115860   C   A   .   PASS    AF=0.869927 ES:SE:LP:AF:SS:ID   -0.000411137:0.000893886:0.190065:0.869927:89866:rs3115860
1   753425  rs3131970   T   C   .   PASS    AF=0.87461  ES:SE:LP:AF:SS:ID   -0.000349364:0.00090846:0.154555:0.87461:89866:rs3131970
1   753541  rs2073813   G   A   .   PASS    AF=0.12951  ES:SE:LP:AF:SS:ID   0.000445479:0.000896619:0.208098:0.12951:89866:rs2073813
1   754105  rs12184325  C   T   .   PASS    AF=0.0357365    ES:SE:LP:AF:SS:ID   -0.000644657:0.00164037:0.158437:0.0357365:89866:rs12184325
1   754182  rs3131969   A   G   .   PASS    AF=0.869574 ES:SE:LP:AF:SS:ID   -0.000416447:0.000893099:0.193138:0.869574:89866:rs3131969
1   754192  rs3131968   A   G   .   PASS    AF=0.869652 ES:SE:LP:AF:SS:ID   -0.000380309:0.000893496:0.173685:0.869652:89866:rs3131968
1   754334  rs3131967   T   C   .   PASS    AF=0.86957  ES:SE:LP:AF:SS:ID   -0.000419303:0.000893063:0.1947:0.86957:89866:rs3131967
1   755890  rs3115858   A   T   .   PASS    AF=0.869566 ES:SE:LP:AF:SS:ID   -0.000436662:0.000891293:0.204682:0.869566:89866:rs3115858
1   756434  rs61768170  G   C   .   PASS    AF=0.126879 ES:SE:LP:AF:SS:ID   0.000433364:0.00090987:0.198001:0.126879:89866:rs61768170
1   756604  rs3131962   A   G   .   PASS    AF=0.869094 ES:SE:LP:AF:SS:ID   -0.000526972:0.000888981:0.257017:0.869094:89866:rs3131962
1   757640  rs3115853   G   A   .   PASS    AF=0.868498 ES:SE:LP:AF:SS:ID   -0.000534783:0.000888437:0.26184:0.868498:89866:rs3115853
1   757734  rs4951929   C   T   .   PASS    AF=0.869245 ES:SE:LP:AF:SS:ID   -0.000490926:0.000889793:0.235724:0.869245:89866:rs4951929
1   757936  rs4951862   C   A   .   PASS    AF=0.869251 ES:SE:LP:AF:SS:ID   -0.000491533:0.000889854:0.236053:0.869251:89866:rs4951862
1   758144  rs3131956   A   G   .   PASS    AF=0.869252 ES:SE:LP:AF:SS:ID   -0.000492546:0.00088986:0.236635:0.869252:89866:rs3131956
1   758626  rs3131954   C   T   .   PASS    AF=0.869724 ES:SE:LP:AF:SS:ID   -0.000462069:0.000892227:0.218574:0.869724:89866:rs3131954
1   759293  rs10157329  T   A   .   PASS    AF=0.0991203    ES:SE:LP:AF:SS:ID   0.0007675:0.0010387:0.337274:0.0991203:89866:rs10157329
1   759837  rs3115851   T   A   .   PASS    AF=0.873732 ES:SE:LP:AF:SS:ID   -0.000305416:0.000905482:0.133185:0.873732:89866:rs3115851
1   761732  rs2286139   C   T   .   PASS    AF=0.863116 ES:SE:LP:AF:SS:ID   -0.000575733:0.000888137:0.286655:0.863116:89866:rs2286139
1   761752  rs1057213   C   T   .   PASS    AF=0.868511 ES:SE:LP:AF:SS:ID   -0.000325182:0.000897011:0.144501:0.868511:89866:rs1057213
1   762273  rs3115849   G   A   .   PASS    AF=0.86539  ES:SE:LP:AF:SS:ID   -0.000331752:0.000897142:0.147799:0.86539:89866:rs3115849
1   762485  rs12095200  C   A   .   PASS    AF=0.0994301    ES:SE:LP:AF:SS:ID   2.40407e-05:0.00107433:0.00782351:0.0994301:89866:rs12095200
1   762589  rs3115848   G   C   .   PASS    AF=0.870696 ES:SE:LP:AF:SS:ID   -0.000311626:0.000907463:0.135907:0.870696:89866:rs3115848
1   762592  rs3131950   C   G   .   PASS    AF=0.870695 ES:SE:LP:AF:SS:ID   -0.000311617:0.000907463:0.135903:0.870695:89866:rs3131950
1   762601  rs3131949   T   C   .   PASS    AF=0.870693 ES:SE:LP:AF:SS:ID   -0.00031122:0.000907466:0.135707:0.870693:89866:rs3131949
1   762632  rs3131948   T   A   .   PASS    AF=0.871061 ES:SE:LP:AF:SS:ID   -0.000331999:0.000907894:0.145934:0.871061:89866:rs3131948
1   764191  rs7515915   T   G   .   PASS    AF=0.126484 ES:SE:LP:AF:SS:ID   0.000385721:0.000908811:0.173111:0.126484:89866:rs7515915
1   766007  rs61768174  A   C   .   PASS    AF=0.106022 ES:SE:LP:AF:SS:ID   0.000582628:0.000987952:0.255417:0.106022:89866:rs61768174
1   766105  rs2519015   T   A   .   PASS    AF=0.854451 ES:SE:LP:AF:SS:ID   -0.000295523:0.000887927:0.131198:0.854451:89866:rs2519015
1   768116  rs376645387 A   AGTTTT  .   PASS    AF=0.839008 ES:SE:LP:AF:SS:ID   -0.000423992:0.000877178:0.201458:0.839008:89866:rs376645387
1   768253  rs2977608   A   C   .   PASS    AF=0.76317  ES:SE:LP:AF:SS:ID   -0.000645889:0.000705244:0.443994:0.76317:89866:rs2977608
1   768448  rs12562034  G   A   .   PASS    AF=0.10519  ES:SE:LP:AF:SS:ID   0.000674128:0.000971936:0.311634:0.10519:89866:rs12562034
1   769138  rs59306077  CAT C   .   PASS    AF=0.129876 ES:SE:LP:AF:SS:ID   0.000475294:0.00089608:0.224881:0.129876:89866:rs762168062
1   769223  rs60320384  C   G   .   PASS    AF=0.129602 ES:SE:LP:AF:SS:ID   0.000535236:0.000894908:0.25981:0.129602:89866:rs60320384
1   769963  rs7518545   G   A   .   PASS    AF=0.104469 ES:SE:LP:AF:SS:ID   0.000813179:0.00097943:0.391051:0.104469:89866:rs7518545
1   770181  rs146076599 A   G   .   PASS    AF=0.00900456   ES:SE:LP:AF:SS:ID   -0.000167707:0.00345626:0.0171411:0.00900456:89866:rs146076599
1   770886  rs371458725 G   A   .   PASS    AF=0.103468 ES:SE:LP:AF:SS:ID   0.000864115:0.000987928:0.418216:0.103468:89866:rs371458725
1   771410  rs2519006   C   T   .   PASS    AF=0.829371 ES:SE:LP:AF:SS:ID   -0.000279932:0.000873499:0.125744:0.829371:89866:rs2519006
1   771823  rs2977605   T   C   .   PASS    AF=0.869112 ES:SE:LP:AF:SS:ID   -0.000496765:0.00089059:0.238834:0.869112:89866:rs2977605