Summary

Summary {data-width=650}

Manhattan plot

manhattan_plot

manhattan_plot

QQ plot

qq_plot

qq_plot

AF plot

af_plot

af_plot

P-Z plot

pz_plot

pz_plot

beta_std plot

beta_std_plot

beta_std_plot

Metadata

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}

LDSC

*********************************************************************
* LD Score Regression (LDSC)
* Version 1.0.1
* (C) 2014-2019 Brendan Bulik-Sullivan and Hilary Finucane
* Broad Institute of MIT and Harvard / MIT Department of Mathematics
* GNU General Public License v3
*********************************************************************
Call: 
./ldsc.py \
--h2 /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-8793/UKB-b-8793_data.vcf.gz \
--ref-ld-chr ../reference/eur_w_ld_chr/ \
--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-8793/ldsc.txt \
--w-ld-chr ../reference/eur_w_ld_chr/ 

Beginning analysis at Thu Oct 17 14:42:04 2019
Reading summary statistics from /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-8793/UKB-b-8793_data.vcf.gz ...
Read summary statistics for 8625255 SNPs.
Dropped 7372 SNPs with duplicated rs numbers.
Reading reference panel LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read reference panel LD Scores for 1290028 SNPs.
Removing partitioned LD Scores with zero variance.
Reading regression weight LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read regression weight LD Scores for 1290028 SNPs.
After merging with reference panel LD, 1285751 SNPs remain.
After merging with regression SNP LD, 1285751 SNPs remain.
Using two-step estimator with cutoff at 30.
Total Observed scale h2: 0.0104 (0.007)
Lambda GC: 1.0239
Mean Chi^2: 1.024
Intercept: 1.011 (0.006)
Ratio: 0.4586 (0.2509)
Analysis finished at Thu Oct 17 14:43:37 2019
Total time elapsed: 1.0m:33.0s

QC metrics

Metrics

Metrics

{
    "af_correlation": 0.9462,
    "inflation_factor": 1,
    "mean_EFFECT": 0,
    "n": "-Inf",
    "n_snps": 9851866,
    "n_clumped_hits": 1,
    "n_p_sig": 1,
    "n_mono": 0,
    "n_ns": 0,
    "n_mac": 0,
    "is_snpid_unique": true,
    "n_miss_EFFECT": 0,
    "n_miss_SE": 0,
    "n_miss_PVAL": 0,
    "n_miss_AF": 0,
    "n_miss_AF_reference": 83101,
    "n_est": "NA",
    "ratio_se_n": "NA",
    "mean_diff": "NaN",
    "ratio_diff": "NaN",
    "sd_y_est1": "NaN",
    "sd_y_est2": "NA",
    "r2_sum1": 0,
    "r2_sum2": 0,
    "r2_sum3": 0,
    "r2_sum4": 0,
    "ldsc_nsnp_merge_refpanel_ld": 1285751,
    "ldsc_nsnp_merge_regression_ld": 1285751,
    "ldsc_observed_scale_h2_beta": 0.0104,
    "ldsc_observed_scale_h2_se": 0.007,
    "ldsc_intercept_beta": 1.011,
    "ldsc_intercept_se": 0.006,
    "ldsc_lambda_gc": 1.0239,
    "ldsc_mean_chisq": 1.024,
    "ldsc_ratio": 0.4583
}

Flags

name value
af_correlation FALSE
inflation_factor FALSE
n TRUE
is_snpid_non_unique FALSE
mean_EFFECT_nonfinite FALSE
mean_EFFECT_05 FALSE
mean_EFFECT_01 FALSE
mean_chisq FALSE
n_p_sig FALSE
miss_EFFECT FALSE
miss_SE FALSE
miss_PVAL FALSE
ldsc_ratio FALSE
ldsc_intercept_beta FALSE
n_clumped_hits FALSE
r2_sum1 FALSE
r2_sum2 FALSE
r2_sum3 FALSE
r2_sum4 FALSE

Definitions

General metrics

  • af_correlation: Correlation coefficient between AF and AF_reference.
  • inflation_factor (lambda): Genomic inflation factor.
  • mean_EFFECT: Mean of EFFECT size.
  • n: Maximum value of reported sample size across all SNPs, \(n\).
  • n_clumped_hits: Number of clumped hits.
  • n_snps: Number of SNPs
  • n_p_sig: Number of SNPs with pvalue below 5e-8.
  • n_mono: Number of monomorphic (MAF == 1 or MAF == 0) SNPs.
  • n_ns: Number of SNPs with nonsense values:
    • alleles other than A, C, G or T.
    • P-values < 0 or > 1.
    • negative or infinite standard errors (<= 0 or = Infinity).
    • infinite beta estimates or allele frequencies < 0 or > 1.
  • n_mac: Number of cases where MAC (\(2 \times N \times MAF\)) is less than 6.
  • is_snpid_unique: true if the combination of ID REF ALT is unique and therefore no duplication in snpid.
  • n_miss_<*>: Number of NA observations for <*> column.

se_n metrics

  • n_est: Estimated sample size value, \(\widehat{n}\).
  • ratio_se_n: \(\texttt{ratio_se_n} = \frac{\sqrt{\widehat{n}}}{\sqrt{n}}\). We expect ratio_se_n to be 1. When it is not 1, it implies that the trait did not have a variance of 1, the reported sample size is wrong, or that the SNP-level effective sample sizes differ markedly from the reported sample size.
  • mean_diff: \(\texttt{mean_diff} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta_j}{\texttt{n_snps}}\), mean difference between the standardised beta, predicted from P-values, and the observed beta. The difference should be very close to zero if trait has a variance of 1.
    • \(\widehat{\beta_j^{std}} = \sqrt{\frac{{z}_j^2 / ({z}_j^2 + n -2)}{2 \times {MAF}_j \times (1 - {MAF}_j)}} \times sign({z}_j)\),
    • \({z}_j = \frac{\beta_j}{{se}_j}\),
    • and \(\beta_j\) is the reported effect size.
  • ratio_diff: \(\texttt{ratio_diff} = |\frac{\texttt{mean_diff}}{\texttt{mean_diff2}}|\), absolute ratio between the mean of diff and the mean of diff2 (expected difference between the standardised beta predicted from P-values, and the standardised beta derived from the observed beta divided by the predicted SD; NOT reported). The ratio should be close to 1. If different from 1, then implies that the betas are not in a standard deviation scale.
    • \(\texttt{mean_diff2} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta^{\prime}_j}{\texttt{n_snps}}\)
    • \(\beta^{\prime}_j = \frac{\beta_j}{\widehat{\texttt{sd2}}_{y}}\)
  • sd_y_est1: The standard deviation for the trait inferred from the reported sample size, median standard errors for the SNP-trait assocations and SNP variances.
    • \(\widehat{\texttt{sd1}}_{y} = \frac{\sqrt{n} \times median({se}_j)}{C}\),
    • \(C = median(\frac{1}{\sqrt{2 \times {MAF}_j \times (1 - {MAF}_j)}})\),
    • and \({se}_j\) is the reported standard error.
  • sd_y_est2: The standard deviation for the trait inferred from the reported sample size, Z statistics for the SNP-trait effects (beta/se) and allele frequency.
    • \(\widehat{\texttt{sd2}}_{y} = median(\widehat{sd_j})\),
    • \(\widehat{sd_j} = \frac{\beta_j}{\widehat{\beta_j^{std}}}\),

r2 metrics

Sum of variance explained, calculated from the clumped top hits sample.

  • r2_sum<*>: r2 statistics under various assumptions
    • 1: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var1}}}\), \(\texttt{var1} = 1\).
    • 2: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var2}}}\), \(\texttt{var2} = {\widehat{\texttt{sd1}}_{y}}^2\),
    • 3: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var3}}}\), \(\texttt{var3} = {\widehat{\texttt{sd2}}_{y}}^2\),
    • 4: \(r^2 = \sum_j{\frac{F_j}{F_j + n - 2}}\), \(F = \frac{\beta_j^2}{{se}_j^2}\).

LDSC metrics

Metrics from LD regression

  • ldsc_nsnp_merge_refpanel_ld: Number of remaining SNPs after merging with reference panel LD.
  • ldsc_nsnp_merge_regression_ld: Number of remaining SNPs after merging with regression SNP LD.
  • ldsc_observed_scale_h2_{beta,se} Coefficient value and SE for total observed scale h2.
  • ldsc_intercept_{beta,se}: Coefficient value and SE for intercept. Intercept is expected to be 1.
  • ldsc_lambda_gc: Lambda GC statistics.
  • ldsc_mean_chisq: Mean \(\chi^2\) statistics.
  • ldsc_ratio: \(\frac{\texttt{ldsc_intercept_beta} - 1}{\texttt{ldsc_mean_chisq} - 1}\), the proportion of the inflation in the mean \(\chi^2\) that the LD Score regression intercepts ascribes to causes other than polygenic heritability. The value of ratio should be close to zero, though in practice values of 0.1-0.2 are not uncommon, probably due to sample/reference LD Score mismatch or model misspecification (e.g., low LD variants have slightly higher \(h^2\) per SNP).

Flags

When a metric needs attention, the flag should return TRUE.

  • af_correlation: abs(af_correlation) < 0.7.
  • inflation_factor: inflation_factor > 1.2.
  • n: n (max reported sample size) < 10000.
  • is_snpid_non_unique: NOT is_snpid_unique.
  • mean_EFFECT_nonfinite: mean(EFFECT) is NA, NaN, or Inf.
  • mean_EFFECT_05: abs(mean(EFFECT)) > 0.5.
  • mean_EFFECT_01: abs(mean(EFFECT)) > 0.1.
  • mean_chisq: ldsc_mean_chisq > 1.3 or ldsc_mean_chisq < 0.7.
  • n_p_sig: n_p_sig > 1000.
  • miss_<*>: n_miss_<*> / n_snps > 0.01.
  • ldsc_ratio: ldsc_ratio > 0.5
  • ldsc_intercept_beta: ldsc_intercept_beta > 1.5
  • n_clumped_hits: n_clumped_hits > 1000
  • r2_sum<*>: r2_sum<*> > 0.5

Plots

  • Manhattan plot
    • Red line: \(-log_{10}^{5 \times 10^{-8}}\)
    • Blue line: \(-log_{10}^{5 \times 10^{-5}}\)
  • QQ plot
  • AF plot
  • P-Z plot
  • beta_std plot: Scatter plot between \(\widehat{\beta_j^{std}}\) and \(\beta_j\)

Diagnostics

Details

Summary stats

skim_type skim_variable n_missing complete_rate character.min character.max character.empty character.n_unique character.whitespace logical.mean logical.count numeric.mean numeric.sd numeric.p0 numeric.p25 numeric.p50 numeric.p75 numeric.p100 numeric.hist
character ID 0 1.0000000 3 58 0 8617917 0 NA NA NA NA NA NA NA NA NA NA
character REF 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
character ALT 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
logical N 8625255 0.0000000 NA NA NA NA NA NaN : NA NA NA NA NA NA NA NA
numeric CHROM 0 1.0000000 NA NA NA NA NA NA NA 8.650335e+00 5.760993e+00 1.0000000 4.000000e+00 8.000000e+00 1.300000e+01 2.200000e+01 ▇▅▅▂▂
numeric POS 0 1.0000000 NA NA NA NA NA NA NA 7.877026e+07 5.637169e+07 828.0000000 3.238547e+07 6.929218e+07 1.145689e+08 2.492385e+08 ▇▆▅▂▁
numeric EFFECT 0 1.0000000 NA NA NA NA NA NA NA 1.710000e-05 1.423480e-02 -0.1342030 -5.953600e-03 -7.090000e-05 5.875200e-03 1.693940e-01 ▁▁▇▁▁
numeric SE 0 1.0000000 NA NA NA NA NA NA NA 1.170450e-02 7.989900e-03 0.0046483 5.474000e-03 8.071200e-03 1.582230e-02 8.012970e-02 ▇▂▁▁▁
numeric PVAL 0 1.0000000 NA NA NA NA NA NA NA 4.972856e-01 2.895259e-01 0.0000000 2.500000e-01 5.000000e-01 7.499995e-01 1.000000e+00 ▇▇▇▇▇
numeric PVAL_ztest 0 1.0000000 NA NA NA NA NA NA NA 4.972859e-01 2.894990e-01 0.0000000 2.458835e-01 4.965277e-01 7.482943e-01 1.000000e+00 ▇▇▇▇▇
numeric AF 0 1.0000000 NA NA NA NA NA NA NA 2.291107e-01 2.594876e-01 0.0053890 2.518600e-02 1.138890e-01 3.626125e-01 9.946110e-01 ▇▂▁▁▁
numeric AF_reference 83101 0.9903654 NA NA NA NA NA NA NA 2.288600e-01 2.514584e-01 0.0000000 2.316290e-02 1.303910e-01 3.596250e-01 1.000000e+00 ▇▂▂▁▁

Head and tail

CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
1 49298 rs200943160 T C -0.0111753 0.0085670 0.1900002 0.1920751 0.623812 0.7821490 NA
1 54676 rs2462492 C T 0.0224866 0.0085420 0.0085000 0.0084768 0.399144 NA NA
1 86028 rs114608975 T C -0.0005237 0.0135976 0.9699999 0.9692763 0.103536 0.0277556 NA
1 91536 rs6702460 G T -0.0026218 0.0084017 0.7499995 0.7549962 0.455916 0.4207270 NA
1 234313 rs8179466 C T 0.0022423 0.0166155 0.8900000 0.8926486 0.074455 NA NA
1 534192 rs6680723 C T 0.0065962 0.0095691 0.4899999 0.4906223 0.242057 NA NA
1 546697 rs12025928 A G 0.0081313 0.0118732 0.4899999 0.4934439 0.912862 NA NA
1 693731 rs12238997 A G 0.0000581 0.0079790 0.9900000 0.9941890 0.117313 0.1417730 NA
1 705882 rs72631875 G A -0.0048184 0.0116302 0.6800001 0.6786564 0.067698 0.0315495 NA
1 706368 rs55727773 A G -0.0061356 0.0059226 0.2999998 0.3002173 0.513304 0.2751600 NA
CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
22 51219006 rs28729663 G A 0.0002015 0.0072033 0.9800000 0.9776857 0.136315 0.2052720 NA
22 51219387 rs9616832 T C -0.0113499 0.0093886 0.2300001 0.2267019 0.071797 0.0654952 NA
22 51219704 rs147475742 G A -0.0150164 0.0124894 0.2300001 0.2292351 0.041190 0.0473243 NA
22 51221190 rs369304721 G A -0.0133868 0.0125780 0.2900000 0.2871916 0.048372 NA NA
22 51221731 rs115055839 T C -0.0103058 0.0093907 0.2700001 0.2724453 0.071348 0.0625000 NA
22 51222100 rs114553188 G T 0.0087725 0.0108818 0.4199997 0.4201486 0.054850 0.0880591 NA
22 51223637 rs375798137 G A 0.0086819 0.0109386 0.4299995 0.4273747 0.054470 0.0788738 NA
22 51229805 rs9616985 T C -0.0107469 0.0094197 0.2500000 0.2539121 0.071253 0.0730831 NA
22 51232488 rs376461333 A G 0.0111187 0.0216953 0.6100002 0.6083057 0.020460 NA NA
22 51237063 rs3896457 T C 0.0114183 0.0056867 0.0449997 0.0446544 0.298393 0.2050720 NA

bcf preview

1   49298   rs10399793  T   C   .   PASS    AF=0.623812 ES:SE:LP:AF:ID  -0.0111753:0.00856696:0.721246:0.623812:rs10399793
1   54676   rs2462492   C   T   .   PASS    AF=0.399144 ES:SE:LP:AF:ID  0.0224866:0.00854204:2.07058:0.399144:rs2462492
1   86028   rs114608975 T   C   .   PASS    AF=0.103536 ES:SE:LP:AF:ID  -0.000523725:0.0135976:0.0132283:0.103536:rs114608975
1   91536   rs6702460   G   T   .   PASS    AF=0.455916 ES:SE:LP:AF:ID  -0.00262182:0.0084017:0.124939:0.455916:rs6702460
1   234313  rs8179466   C   T   .   PASS    AF=0.074455 ES:SE:LP:AF:ID  0.00224232:0.0166155:0.05061:0.074455:rs8179466
1   534192  rs6680723   C   T   .   PASS    AF=0.242057 ES:SE:LP:AF:ID  0.00659616:0.00956909:0.309804:0.242057:rs6680723
1   546697  rs12025928  A   G   .   PASS    AF=0.912862 ES:SE:LP:AF:ID  0.00813126:0.0118732:0.309804:0.912862:rs12025928
1   693731  rs12238997  A   G   .   PASS    AF=0.117313 ES:SE:LP:AF:ID  5.8112e-05:0.007979:0.00436481:0.117313:rs12238997
1   705882  rs72631875  G   A   .   PASS    AF=0.067698 ES:SE:LP:AF:ID  -0.00481836:0.0116302:0.167491:0.067698:rs72631875
1   706368  rs12029736  A   G   .   PASS    AF=0.513304 ES:SE:LP:AF:ID  -0.00613559:0.00592257:0.522879:0.513304:rs12029736
1   714596  rs149887893 T   C   .   PASS    AF=0.033677 ES:SE:LP:AF:ID  -0.00245164:0.0147623:0.0604807:0.033677:rs149887893
1   715265  rs12184267  C   T   .   PASS    AF=0.037457 ES:SE:LP:AF:ID  0.00546972:0.0133886:0.167491:0.037457:rs12184267
1   715367  rs12184277  A   G   .   PASS    AF=0.037644 ES:SE:LP:AF:ID  0.00394315:0.0133186:0.113509:0.037644:rs12184277
1   717485  rs12184279  C   A   .   PASS    AF=0.03722  ES:SE:LP:AF:ID  0.0046248:0.0134403:0.136677:0.03722:rs12184279
1   717587  rs144155419 G   A   .   PASS    AF=0.016282 ES:SE:LP:AF:ID  -0.0183731:0.021082:0.420216:0.016282:rs144155419
1   720381  rs116801199 G   T   .   PASS    AF=0.03786  ES:SE:LP:AF:ID  0.00410669:0.0132731:0.119186:0.03786:rs116801199
1   721290  rs12565286  G   C   .   PASS    AF=0.037955 ES:SE:LP:AF:ID  0.00412057:0.013231:0.119186:0.037955:rs12565286
1   722670  rs116030099 T   C   .   PASS    AF=0.102736 ES:SE:LP:AF:ID  0.00292787:0.00966489:0.119186:0.102736:rs116030099
1   723891  rs2977670   G   C   .   PASS    AF=0.95809  ES:SE:LP:AF:ID  -0.00853289:0.0127791:0.30103:0.95809:rs2977670
1   724849  rs12126395  C   A   .   PASS    AF=0.03169  ES:SE:LP:AF:ID  -0.0145852:0.0234006:0.275724:0.03169:rs12126395
1   725060  rs865924913 A   T   .   PASS    AF=0.052723 ES:SE:LP:AF:ID  0.000514106:0.0188485:0.00877392:0.052723:rs865924913
1   726794  rs28454925  C   G   .   PASS    AF=0.037449 ES:SE:LP:AF:ID  0.00111611:0.0133178:0.0315171:0.037449:rs28454925
1   729632  rs116720794 C   T   .   PASS    AF=0.037717 ES:SE:LP:AF:ID  0.00183803:0.0132075:0.05061:0.037717:rs116720794
1   729679  rs4951859   C   G   .   PASS    AF=0.841441 ES:SE:LP:AF:ID  -0.00205737:0.00690036:0.113509:0.841441:rs4951859
1   730087  rs148120343 T   C   .   PASS    AF=0.056334 ES:SE:LP:AF:ID  -0.00326125:0.0112092:0.113509:0.056334:rs148120343
1   731718  rs58276399  T   C   .   PASS    AF=0.123078 ES:SE:LP:AF:ID  0.00166841:0.00757913:0.0809219:0.123078:rs58276399
1   732989  rs369030935 C   T   .   PASS    AF=0.02513  ES:SE:LP:AF:ID  0.00271571:0.0188746:0.05061:0.02513:rs369030935
1   734349  rs141242758 T   C   .   PASS    AF=0.12233  ES:SE:LP:AF:ID  0.0011089:0.00758157:0.0555173:0.12233:rs141242758
1   736289  rs79010578  T   A   .   PASS    AF=0.134139 ES:SE:LP:AF:ID  0.00281534:0.00744254:0.148742:0.134139:rs79010578
1   736689  rs181876450 T   C   .   PASS    AF=0.011558 ES:SE:LP:AF:ID  0.0151931:0.0265256:0.244125:0.011558:rs181876450
1   740284  rs61770167  C   T   .   PASS    AF=0.0061   ES:SE:LP:AF:ID  -0.0318477:0.0336842:0.468521:0.0061:rs61770167
1   752478  rs146277091 G   A   .   PASS    AF=0.0376   ES:SE:LP:AF:ID  0.00285498:0.0130851:0.0809219:0.0376:rs146277091
1   752566  rs3094315   G   A   .   PASS    AF=0.837029 ES:SE:LP:AF:ID  0.000890001:0.00667526:0.05061:0.837029:rs3094315
1   752721  rs3131972   A   G   .   PASS    AF=0.836733 ES:SE:LP:AF:ID  4.88734e-05:0.00667036:0.00436481:0.836733:rs3131972
1   753405  rs3115860   C   A   .   PASS    AF=0.868562 ES:SE:LP:AF:ID  0.00174381:0.00716765:0.091515:0.868562:rs3115860
1   753541  rs2073813   G   A   .   PASS    AF=0.131004 ES:SE:LP:AF:ID  -0.00137818:0.00718606:0.0705811:0.131004:rs2073813
1   754063  rs12184312  G   T   .   PASS    AF=0.038045 ES:SE:LP:AF:ID  0.00384718:0.0128799:0.113509:0.038045:rs12184312
1   754105  rs12184325  C   T   .   PASS    AF=0.038292 ES:SE:LP:AF:ID  0.00461729:0.0128:0.142668:0.038292:rs12184325
1   754182  rs3131969   A   G   .   PASS    AF=0.867976 ES:SE:LP:AF:ID  0.00119832:0.00715612:0.0604807:0.867976:rs3131969
1   754192  rs3131968   A   G   .   PASS    AF=0.86805  ES:SE:LP:AF:ID  0.000993681:0.00715905:0.05061:0.86805:rs3131968
1   754211  rs12184313  G   A   .   PASS    AF=0.038199 ES:SE:LP:AF:ID  0.00381008:0.0128592:0.113509:0.038199:rs12184313
1   754334  rs3131967   T   C   .   PASS    AF=0.867987 ES:SE:LP:AF:ID  0.00119152:0.00715595:0.0604807:0.867987:rs3131967
1   754433  rs150578204 G   A   .   PASS    AF=0.005396 ES:SE:LP:AF:ID  -0.0512298:0.0358653:0.823909:0.005396:rs150578204
1   754503  rs3115859   G   A   .   PASS    AF=0.836159 ES:SE:LP:AF:ID  0.0010276:0.00665084:0.0555173:0.836159:rs3115859
1   754629  rs10454459  A   G   .   PASS    AF=0.038203 ES:SE:LP:AF:ID  0.00420639:0.0128779:0.130768:0.038203:rs10454459
1   754964  rs3131966   C   T   .   PASS    AF=0.836793 ES:SE:LP:AF:ID  0.00105221:0.00666906:0.0604807:0.836793:rs3131966
1   755240  rs181660517 T   G   .   PASS    AF=0.01303  ES:SE:LP:AF:ID  -0.025638:0.024128:0.537602:0.01303:rs181660517
1   755435  rs184270342 T   G   .   PASS    AF=0.005662 ES:SE:LP:AF:ID  0.0590945:0.0358183:1.00436:0.005662:rs184270342
1   755775  rs3131965   A   G   .   PASS    AF=0.838109 ES:SE:LP:AF:ID  0.00136454:0.00676294:0.0757207:0.838109:rs3131965
1   755890  rs3115858   A   T   .   PASS    AF=0.868228 ES:SE:LP:AF:ID  0.00222572:0.00714673:0.119186:0.868228:rs3115858