Summary

Summary {data-width=650}

Manhattan plot

manhattan_plot

manhattan_plot

QQ plot

qq_plot

qq_plot

AF plot

af_plot

af_plot

P-Z plot

pz_plot

pz_plot

beta_std plot

beta_std_plot

beta_std_plot

Metadata

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    "gwas_harmonisation_command": "--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/vcf_09_19b/bgzip_vcf/data.batch_2764.vcf.gz --id UKB-b:8727 --data /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/raw-output/data.batch_2764.txt.gz --cohort_controls 170248 --ref /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/human_g1k_v37.fasta --json /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/ukb_gwas.json; 1.1.1",
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}
 

LDSC

*********************************************************************
* LD Score Regression (LDSC)
* Version 1.0.1
* (C) 2014-2019 Brendan Bulik-Sullivan and Hilary Finucane
* Broad Institute of MIT and Harvard / MIT Department of Mathematics
* GNU General Public License v3
*********************************************************************
Call: 
./ldsc.py \
--h2 /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-8727/UKB-b-8727_data.vcf.gz \
--ref-ld-chr ../reference/eur_w_ld_chr/ \
--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-8727/ldsc.txt \
--w-ld-chr ../reference/eur_w_ld_chr/ 

Beginning analysis at Thu Oct 17 14:42:04 2019
Reading summary statistics from /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-8727/UKB-b-8727_data.vcf.gz ...
Read summary statistics for 9394096 SNPs.
Dropped 10902 SNPs with duplicated rs numbers.
Reading reference panel LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read reference panel LD Scores for 1290028 SNPs.
Removing partitioned LD Scores with zero variance.
Reading regression weight LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read regression weight LD Scores for 1290028 SNPs.
After merging with reference panel LD, 1288146 SNPs remain.
After merging with regression SNP LD, 1288146 SNPs remain.
Using two-step estimator with cutoff at 30.
Total Observed scale h2: 0.0806 (0.0045)
Lambda GC: 1.2717
Mean Chi^2: 1.3153
Intercept: 1.0444 (0.0079)
Ratio: 0.1407 (0.025)
Analysis finished at Thu Oct 17 14:43:41 2019
Total time elapsed: 1.0m:36.03s

QC metrics

Metrics

Metrics

{
    "af_correlation": 0.9488,
    "inflation_factor": 1.1999,
    "mean_EFFECT": -0,
    "n": "-Inf",
    "n_snps": 9851866,
    "n_clumped_hits": 6,
    "n_p_sig": 540,
    "n_mono": 0,
    "n_ns": 0,
    "n_mac": 0,
    "is_snpid_unique": true,
    "n_miss_EFFECT": 0,
    "n_miss_SE": 0,
    "n_miss_PVAL": 0,
    "n_miss_AF": 0,
    "n_miss_AF_reference": 121499,
    "n_est": "NA",
    "ratio_se_n": "NA",
    "mean_diff": "NaN",
    "ratio_diff": "NaN",
    "sd_y_est1": "NaN",
    "sd_y_est2": "NA",
    "r2_sum1": 0,
    "r2_sum2": 0,
    "r2_sum3": 0,
    "r2_sum4": 0,
    "ldsc_nsnp_merge_refpanel_ld": 1288146,
    "ldsc_nsnp_merge_regression_ld": 1288146,
    "ldsc_observed_scale_h2_beta": 0.0806,
    "ldsc_observed_scale_h2_se": 0.0045,
    "ldsc_intercept_beta": 1.0444,
    "ldsc_intercept_se": 0.0079,
    "ldsc_lambda_gc": 1.2717,
    "ldsc_mean_chisq": 1.3153,
    "ldsc_ratio": 0.1408
}
 

Flags

name value
af_correlation FALSE
inflation_factor FALSE
n TRUE
is_snpid_non_unique FALSE
mean_EFFECT_nonfinite FALSE
mean_EFFECT_05 FALSE
mean_EFFECT_01 FALSE
mean_chisq TRUE
n_p_sig FALSE
miss_EFFECT FALSE
miss_SE FALSE
miss_PVAL FALSE
ldsc_ratio FALSE
ldsc_intercept_beta FALSE
n_clumped_hits FALSE
r2_sum1 FALSE
r2_sum2 FALSE
r2_sum3 FALSE
r2_sum4 FALSE

Definitions

General metrics

  • af_correlation: Correlation coefficient between AF and AF_reference.
  • inflation_factor (lambda): Genomic inflation factor.
  • mean_EFFECT: Mean of EFFECT size.
  • n: Maximum value of reported sample size across all SNPs, \(n\).
  • n_clumped_hits: Number of clumped hits.
  • n_snps: Number of SNPs
  • n_p_sig: Number of SNPs with pvalue below 5e-8.
  • n_mono: Number of monomorphic (MAF == 1 or MAF == 0) SNPs.
  • n_ns: Number of SNPs with nonsense values:
    • alleles other than A, C, G or T.
    • P-values < 0 or > 1.
    • negative or infinite standard errors (<= 0 or = Infinity).
    • infinite beta estimates or allele frequencies < 0 or > 1.
  • n_mac: Number of cases where MAC (\(2 \times N \times MAF\)) is less than 6.
  • is_snpid_unique: true if the combination of ID REF ALT is unique and therefore no duplication in snpid.
  • n_miss_<*>: Number of NA observations for <*> column.

se_n metrics

  • n_est: Estimated sample size value, \(\widehat{n}\).
  • ratio_se_n: \(\texttt{ratio_se_n} = \frac{\sqrt{\widehat{n}}}{\sqrt{n}}\). We expect ratio_se_n to be 1. When it is not 1, it implies that the trait did not have a variance of 1, the reported sample size is wrong, or that the SNP-level effective sample sizes differ markedly from the reported sample size.
  • mean_diff: \(\texttt{mean_diff} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta_j}{\texttt{n_snps}}\), mean difference between the standardised beta, predicted from P-values, and the observed beta. The difference should be very close to zero if trait has a variance of 1.
    • \(\widehat{\beta_j^{std}} = \sqrt{\frac{{z}_j^2 / ({z}_j^2 + n -2)}{2 \times {MAF}_j \times (1 - {MAF}_j)}} \times sign({z}_j)\),
    • \({z}_j = \frac{\beta_j}{{se}_j}\),
    • and \(\beta_j\) is the reported effect size.
  • ratio_diff: \(\texttt{ratio_diff} = |\frac{\texttt{mean_diff}}{\texttt{mean_diff2}}|\), absolute ratio between the mean of diff and the mean of diff2 (expected difference between the standardised beta predicted from P-values, and the standardised beta derived from the observed beta divided by the predicted SD; NOT reported). The ratio should be close to 1. If different from 1, then implies that the betas are not in a standard deviation scale.
    • \(\texttt{mean_diff2} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta^{\prime}_j}{\texttt{n_snps}}\)
    • \(\beta^{\prime}_j = \frac{\beta_j}{\widehat{\texttt{sd2}}_{y}}\)
  • sd_y_est1: The standard deviation for the trait inferred from the reported sample size, median standard errors for the SNP-trait assocations and SNP variances.
    • \(\widehat{\texttt{sd1}}_{y} = \frac{\sqrt{n} \times median({se}_j)}{C}\),
    • \(C = median(\frac{1}{\sqrt{2 \times {MAF}_j \times (1 - {MAF}_j)}})\),
    • and \({se}_j\) is the reported standard error.
  • sd_y_est2: The standard deviation for the trait inferred from the reported sample size, Z statistics for the SNP-trait effects (beta/se) and allele frequency.
    • \(\widehat{\texttt{sd2}}_{y} = median(\widehat{sd_j})\),
    • \(\widehat{sd_j} = \frac{\beta_j}{\widehat{\beta_j^{std}}}\),

r2 metrics

Sum of variance explained, calculated from the clumped top hits sample.

  • r2_sum<*>: r2 statistics under various assumptions
    • 1: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var1}}}\), \(\texttt{var1} = 1\).
    • 2: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var2}}}\), \(\texttt{var2} = {\widehat{\texttt{sd1}}_{y}}^2\),
    • 3: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var3}}}\), \(\texttt{var3} = {\widehat{\texttt{sd2}}_{y}}^2\),
    • 4: \(r^2 = \sum_j{\frac{F_j}{F_j + n - 2}}\), \(F = \frac{\beta_j^2}{{se}_j^2}\).

LDSC metrics

Metrics from LD regression

  • ldsc_nsnp_merge_refpanel_ld: Number of remaining SNPs after merging with reference panel LD.
  • ldsc_nsnp_merge_regression_ld: Number of remaining SNPs after merging with regression SNP LD.
  • ldsc_observed_scale_h2_{beta,se} Coefficient value and SE for total observed scale h2.
  • ldsc_intercept_{beta,se}: Coefficient value and SE for intercept. Intercept is expected to be 1.
  • ldsc_lambda_gc: Lambda GC statistics.
  • ldsc_mean_chisq: Mean \(\chi^2\) statistics.
  • ldsc_ratio: \(\frac{\texttt{ldsc_intercept_beta} - 1}{\texttt{ldsc_mean_chisq} - 1}\), the proportion of the inflation in the mean \(\chi^2\) that the LD Score regression intercepts ascribes to causes other than polygenic heritability. The value of ratio should be close to zero, though in practice values of 0.1-0.2 are not uncommon, probably due to sample/reference LD Score mismatch or model misspecification (e.g., low LD variants have slightly higher \(h^2\) per SNP).

Flags

When a metric needs attention, the flag should return TRUE.

  • af_correlation: abs(af_correlation) < 0.7.
  • inflation_factor: inflation_factor > 1.2.
  • n: n (max reported sample size) < 10000.
  • is_snpid_non_unique: NOT is_snpid_unique.
  • mean_EFFECT_nonfinite: mean(EFFECT) is NA, NaN, or Inf.
  • mean_EFFECT_05: abs(mean(EFFECT)) > 0.5.
  • mean_EFFECT_01: abs(mean(EFFECT)) > 0.1.
  • mean_chisq: ldsc_mean_chisq > 1.3 or ldsc_mean_chisq < 0.7.
  • n_p_sig: n_p_sig > 1000.
  • miss_<*>: n_miss_<*> / n_snps > 0.01.
  • ldsc_ratio: ldsc_ratio > 0.5
  • ldsc_intercept_beta: ldsc_intercept_beta > 1.5
  • n_clumped_hits: n_clumped_hits > 1000
  • r2_sum<*>: r2_sum<*> > 0.5

Plots

  • Manhattan plot
    • Red line: \(-log_{10}^{5 \times 10^{-8}}\)
    • Blue line: \(-log_{10}^{5 \times 10^{-5}}\)
  • QQ plot
  • AF plot
  • P-Z plot
  • beta_std plot: Scatter plot between \(\widehat{\beta_j^{std}}\) and \(\beta_j\)

Diagnostics

Details

Summary stats

skim_type skim_variable n_missing complete_rate character.min character.max character.empty character.n_unique character.whitespace logical.mean logical.count numeric.mean numeric.sd numeric.p0 numeric.p25 numeric.p50 numeric.p75 numeric.p100 numeric.hist
character ID 0 1.0000000 3 58 0 9383250 0 NA NA NA NA NA NA NA NA NA NA
character REF 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
character ALT 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
logical N 9394096 0.0000000 NA NA NA NA NA NaN : NA NA NA NA NA NA NA NA
numeric CHROM 0 1.0000000 NA NA NA NA NA NA NA 8.631408e+00 5.752726e+00 1.000000 4.000000e+00 8.000000e+00 1.300000e+01 2.200000e+01 ▇▅▃▂▂
numeric POS 0 1.0000000 NA NA NA NA NA NA NA 7.882518e+07 5.630561e+07 828.000000 3.252208e+07 6.941152e+07 1.145533e+08 2.492385e+08 ▇▆▅▂▁
numeric EFFECT 0 1.0000000 NA NA NA NA NA NA NA -2.990000e-05 1.339290e-02 -0.166215 -5.045700e-03 -3.620000e-05 4.976600e-03 2.011280e-01 ▁▁▇▁▁
numeric SE 0 1.0000000 NA NA NA NA NA NA NA 9.987100e-03 8.099900e-03 0.003253 3.935500e-03 6.273300e-03 1.365070e-02 1.166810e-01 ▇▁▁▁▁
numeric PVAL 0 1.0000000 NA NA NA NA NA NA NA 4.706396e-01 2.963533e-01 0.000000 2.099999e-01 4.600002e-01 7.300002e-01 1.000000e+00 ▇▇▇▆▆
numeric PVAL_ztest 0 1.0000000 NA NA NA NA NA NA NA 4.706409e-01 2.963279e-01 0.000000 2.068138e-01 4.603515e-01 7.269624e-01 9.999998e-01 ▇▆▆▆▆
numeric AF 0 1.0000000 NA NA NA NA NA NA NA 2.119266e-01 2.575764e-01 0.002056 1.667800e-02 8.986000e-02 3.322400e-01 9.979440e-01 ▇▂▁▁▁
numeric AF_reference 121499 0.9870665 NA NA NA NA NA NA NA 2.132154e-01 2.493423e-01 0.000000 1.417730e-02 1.088260e-01 3.328670e-01 1.000000e+00 ▇▂▁▁▁

Head and tail

CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
1 49298 rs200943160 T C -0.0151213 0.0059984 0.0120000 0.0117056 0.623587 0.7821490 NA
1 54676 rs2462492 C T -0.0004300 0.0059253 0.9400001 0.9421476 0.401333 NA NA
1 86028 rs114608975 T C 0.0049772 0.0095144 0.5999997 0.6008906 0.103589 0.0277556 NA
1 91536 rs6702460 G T -0.0094577 0.0058440 0.1100001 0.1055866 0.457079 0.4207270 NA
1 234313 rs8179466 C T -0.0020823 0.0116028 0.8600001 0.8575717 0.074274 NA NA
1 534192 rs6680723 C T 0.0025358 0.0066832 0.6999999 0.7043672 0.241088 NA NA
1 546697 rs12025928 A G -0.0146554 0.0083544 0.0790005 0.0793922 0.913745 NA NA
1 693731 rs12238997 A G 0.0013673 0.0055885 0.8100000 0.8067166 0.116908 0.1417730 NA
1 705882 rs72631875 G A 0.0119938 0.0082702 0.1499999 0.1469883 0.066476 0.0315495 NA
1 706368 rs55727773 A G -0.0007932 0.0041518 0.8499999 0.8484932 0.514834 0.2751600 NA
CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
22 51219006 rs28729663 G A 0.0011491 0.0050162 0.8200001 0.8188107 0.138184 0.2052720 NA
22 51219387 rs9616832 T C 0.0025029 0.0065122 0.6999999 0.7007266 0.073662 0.0654952 NA
22 51219704 rs147475742 G A 0.0052437 0.0087298 0.5500004 0.5480669 0.041874 0.0473243 NA
22 51221190 rs369304721 G A 0.0027514 0.0087199 0.7499995 0.7523585 0.049542 NA NA
22 51221731 rs115055839 T C 0.0018724 0.0065138 0.7700005 0.7737645 0.073198 0.0625000 NA
22 51222100 rs114553188 G T -0.0009772 0.0076338 0.9000000 0.8981416 0.054886 0.0880591 NA
22 51223637 rs375798137 G A -0.0007198 0.0076714 0.9299999 0.9252454 0.054506 0.0788738 NA
22 51229805 rs9616985 T C 0.0023675 0.0065364 0.7199992 0.7172043 0.073074 0.0730831 NA
22 51232488 rs376461333 A G 0.0101409 0.0152154 0.5099998 0.5050985 0.020339 NA NA
22 51237063 rs3896457 T C -0.0070834 0.0039892 0.0759994 0.0757919 0.298331 0.2050720 NA

bcf preview

1   49298   rs10399793  T   C   .   PASS    AF=0.623587 ES:SE:LP:AF:ID  -0.0151213:0.00599838:1.92082:0.623587:rs10399793
1   54676   rs2462492   C   T   .   PASS    AF=0.401333 ES:SE:LP:AF:ID  -0.000430002:0.00592527:0.0268721:0.401333:rs2462492
1   86028   rs114608975 T   C   .   PASS    AF=0.103589 ES:SE:LP:AF:ID  0.00497717:0.00951439:0.221849:0.103589:rs114608975
1   91536   rs6702460   G   T   .   PASS    AF=0.457079 ES:SE:LP:AF:ID  -0.00945767:0.00584401:0.958607:0.457079:rs6702460
1   234313  rs8179466   C   T   .   PASS    AF=0.074274 ES:SE:LP:AF:ID  -0.00208231:0.0116028:0.0655015:0.074274:rs8179466
1   534192  rs6680723   C   T   .   PASS    AF=0.241088 ES:SE:LP:AF:ID  0.0025358:0.00668315:0.154902:0.241088:rs6680723
1   546697  rs12025928  A   G   .   PASS    AF=0.913745 ES:SE:LP:AF:ID  -0.0146554:0.00835435:1.10237:0.913745:rs12025928
1   693731  rs12238997  A   G   .   PASS    AF=0.116908 ES:SE:LP:AF:ID  0.0013673:0.00558848:0.091515:0.116908:rs12238997
1   705882  rs72631875  G   A   .   PASS    AF=0.066476 ES:SE:LP:AF:ID  0.0119938:0.00827015:0.823909:0.066476:rs72631875
1   706368  rs12029736  A   G   .   PASS    AF=0.514834 ES:SE:LP:AF:ID  -0.000793173:0.00415184:0.0705811:0.514834:rs12029736
1   714596  rs149887893 T   C   .   PASS    AF=0.032984 ES:SE:LP:AF:ID  0.00735974:0.0104576:0.318759:0.032984:rs149887893
1   715265  rs12184267  C   T   .   PASS    AF=0.03657  ES:SE:LP:AF:ID  0.0081046:0.00950884:0.408935:0.03657:rs12184267
1   715367  rs12184277  A   G   .   PASS    AF=0.036652 ES:SE:LP:AF:ID  0.00762297:0.00947805:0.376751:0.036652:rs12184277
1   717485  rs12184279  C   A   .   PASS    AF=0.036338 ES:SE:LP:AF:ID  0.00762184:0.00954917:0.376751:0.036338:rs12184279
1   717587  rs144155419 G   A   .   PASS    AF=0.016288 ES:SE:LP:AF:ID  0.0244642:0.0147135:1.01773:0.016288:rs144155419
1   720381  rs116801199 G   T   .   PASS    AF=0.036873 ES:SE:LP:AF:ID  0.0069396:0.00944347:0.337242:0.036873:rs116801199
1   721290  rs12565286  G   C   .   PASS    AF=0.036966 ES:SE:LP:AF:ID  0.00699746:0.00941185:0.337242:0.036966:rs12565286
1   722670  rs116030099 T   C   .   PASS    AF=0.101103 ES:SE:LP:AF:ID  -0.00632513:0.00684962:0.443698:0.101103:rs116030099
1   723891  rs2977670   G   C   .   PASS    AF=0.959315 ES:SE:LP:AF:ID  -0.00953844:0.00908995:0.537602:0.959315:rs2977670
1   724849  rs12126395  C   A   .   PASS    AF=0.031557 ES:SE:LP:AF:ID  -0.00848455:0.0163032:0.221849:0.031557:rs12126395
1   725060  rs865924913 A   T   .   PASS    AF=0.053487 ES:SE:LP:AF:ID  0.00637563:0.01303:0.207608:0.053487:rs865924913
1   726794  rs28454925  C   G   .   PASS    AF=0.036507 ES:SE:LP:AF:ID  0.0049096:0.0094675:0.221849:0.036507:rs28454925
1   729632  rs116720794 C   T   .   PASS    AF=0.036845 ES:SE:LP:AF:ID  0.00540068:0.00938078:0.251812:0.036845:rs116720794
1   729679  rs4951859   C   G   .   PASS    AF=0.842876 ES:SE:LP:AF:ID  -0.00218003:0.00485409:0.187087:0.842876:rs4951859
1   730087  rs148120343 T   C   .   PASS    AF=0.056068 ES:SE:LP:AF:ID  -0.00621556:0.00784649:0.366532:0.056068:rs148120343
1   731718  rs58276399  T   C   .   PASS    AF=0.122868 ES:SE:LP:AF:ID  -0.000973152:0.00530259:0.0705811:0.122868:rs58276399
1   732989  rs369030935 C   T   .   PASS    AF=0.025821 ES:SE:LP:AF:ID  0.0137539:0.0130279:0.537602:0.025821:rs369030935
1   734349  rs141242758 T   C   .   PASS    AF=0.122066 ES:SE:LP:AF:ID  -0.000846:0.00530601:0.0604807:0.122066:rs141242758
1   736289  rs79010578  T   A   .   PASS    AF=0.132961 ES:SE:LP:AF:ID  -0.000957368:0.00522748:0.0705811:0.132961:rs79010578
1   736689  rs181876450 T   C   .   PASS    AF=0.011192 ES:SE:LP:AF:ID  0.0116102:0.0189822:0.267606:0.011192:rs181876450
1   740284  rs61770167  C   T   .   PASS    AF=0.005853 ES:SE:LP:AF:ID  -0.00246024:0.0241161:0.0362122:0.005853:rs61770167
1   742813  rs112573343 C   T   .   PASS    AF=0.002155 ES:SE:LP:AF:ID  0.0295879:0.042467:0.309804:0.002155:rs112573343
1   752478  rs146277091 G   A   .   PASS    AF=0.036766 ES:SE:LP:AF:ID  0.00655112:0.00928587:0.318759:0.036766:rs146277091
1   752566  rs3094315   G   A   .   PASS    AF=0.838652 ES:SE:LP:AF:ID  -0.00399525:0.00470093:0.39794:0.838652:rs3094315
1   752721  rs3131972   A   G   .   PASS    AF=0.838284 ES:SE:LP:AF:ID  -0.00420469:0.00469621:0.431798:0.838284:rs3131972
1   753405  rs3115860   C   A   .   PASS    AF=0.869449 ES:SE:LP:AF:ID  -0.00282743:0.00503594:0.244125:0.869449:rs3115860
1   753541  rs2073813   G   A   .   PASS    AF=0.130214 ES:SE:LP:AF:ID  0.00342264:0.00504632:0.30103:0.130214:rs2073813
1   754063  rs12184312  G   T   .   PASS    AF=0.037271 ES:SE:LP:AF:ID  0.0050784:0.00912859:0.236572:0.037271:rs12184312
1   754105  rs12184325  C   T   .   PASS    AF=0.03754  ES:SE:LP:AF:ID  0.00470833:0.00906612:0.221849:0.03754:rs12184325
1   754182  rs3131969   A   G   .   PASS    AF=0.868802 ES:SE:LP:AF:ID  -0.00310392:0.00502729:0.267606:0.868802:rs3131969
1   754192  rs3131968   A   G   .   PASS    AF=0.868871 ES:SE:LP:AF:ID  -0.00294656:0.00502911:0.251812:0.868871:rs3131968
1   754211  rs12184313  G   A   .   PASS    AF=0.037481 ES:SE:LP:AF:ID  0.00515074:0.00910829:0.244125:0.037481:rs12184313
1   754334  rs3131967   T   C   .   PASS    AF=0.868797 ES:SE:LP:AF:ID  -0.00299959:0.00502717:0.259637:0.868797:rs3131967
1   754433  rs150578204 G   A   .   PASS    AF=0.004916 ES:SE:LP:AF:ID  0.0135011:0.0263997:0.21467:0.004916:rs150578204
1   754458  rs142682604 G   T   .   PASS    AF=0.004884 ES:SE:LP:AF:ID  0.0133175:0.0264679:0.21467:0.004884:rs142682604
1   754503  rs3115859   G   A   .   PASS    AF=0.837721 ES:SE:LP:AF:ID  -0.00451839:0.00468249:0.481486:0.837721:rs3115859
1   754629  rs10454459  A   G   .   PASS    AF=0.037499 ES:SE:LP:AF:ID  0.00592899:0.00912163:0.283997:0.037499:rs10454459
1   754964  rs3131966   C   T   .   PASS    AF=0.838346 ES:SE:LP:AF:ID  -0.00444312:0.00469543:0.468521:0.838346:rs3131966
1   755240  rs181660517 T   G   .   PASS    AF=0.013805 ES:SE:LP:AF:ID  -0.0191119:0.0163585:0.619789:0.013805:rs181660517
1   755435  rs184270342 T   G   .   PASS    AF=0.005636 ES:SE:LP:AF:ID  0.010824:0.0251028:0.173925:0.005636:rs184270342