Summary

Summary {data-width=650}

Manhattan plot

manhattan_plot

manhattan_plot

QQ plot

qq_plot

qq_plot

AF plot

af_plot

af_plot

P-Z plot

pz_plot

pz_plot

beta_std plot

beta_std_plot

beta_std_plot

Metadata

{
    "fileformat": "VCFv4.2",
    "FILTER": "<ID=PASS,Description=\"All filters passed\">",
    "INFO": "<ID=AF,Number=A,Type=Float,Description=\"Allele Frequency\">",
    "FORMAT": "<ID=ES,Number=A,Type=Float,Description=\"Effect size estimate relative to the alternative allele\">",
    "FORMAT.1": "<ID=SE,Number=A,Type=Float,Description=\"Standard error of effect size estimate\">",
    "FORMAT.2": "<ID=LP,Number=A,Type=Float,Description=\"-log10 p-value for effect estimate\">",
    "FORMAT.3": "<ID=AF,Number=A,Type=Float,Description=\"Alternate allele frequency in the association study\">",
    "FORMAT.4": "<ID=SS,Number=A,Type=Float,Description=\"Sample size used to estimate genetic effect\">",
    "FORMAT.5": "<ID=EZ,Number=A,Type=Float,Description=\"Z-score provided if it was used to derive the EFFECT and SE fields\">",
    "FORMAT.6": "<ID=SI,Number=A,Type=Float,Description=\"Accuracy score of summary data imputation\">",
    "FORMAT.7": "<ID=NC,Number=A,Type=Float,Description=\"Number of cases used to estimate genetic effect\">",
    "FORMAT.8": "<ID=ID,Number=1,Type=String,Description=\"Study variant identifier\">",
    "META": "<ID=TotalVariants,Number=1,Type=Integer,Description=\"Total number of variants in input\">",
    "META.1": "<ID=VariantsNotRead,Number=1,Type=Integer,Description=\"Number of variants that could not be read\">",
    "META.2": "<ID=HarmonisedVariants,Number=1,Type=Integer,Description=\"Total number of harmonised variants\">",
    "META.3": "<ID=VariantsNotHarmonised,Number=1,Type=Integer,Description=\"Total number of variants that could not be harmonised\">",
    "META.4": "<ID=SwitchedAlleles,Number=1,Type=Integer,Description=\"Total number of variants strand switched\">",
    "META.5": "<ID=TotalControls,Number=1,Type=Integer,Description=\"Total number of controls in the association study\">",
    "META.6": "<ID=TotalCases,Number=1,Type=Integer,Description=\"Total number of cases in the association study\">",
    "META.7": "<ID=StudyType,Number=1,Type=String,Description=\"Type of GWAS study [Continuous or CaseControl]\">",
    "SAMPLE": "<ID=UKB-b:6519,TotalVariants=9851866,VariantsNotRead=0,HarmonisedVariants=9851866,VariantsNotHarmonised=0,SwitchedAlleles=9851866,TotalControls=194953,TotalCases=255812,StudyType=CaseControl>",
    "contig": "<ID=1,length=249250621,assembly=GRCh37>",
    "contig.1": "<ID=2,length=243199373,assembly=GRCh37>",
    "contig.2": "<ID=3,length=198022430,assembly=GRCh37>",
    "contig.3": "<ID=4,length=191154276,assembly=GRCh37>",
    "contig.4": "<ID=5,length=180915260,assembly=GRCh37>",
    "contig.5": "<ID=6,length=171115067,assembly=GRCh37>",
    "contig.6": "<ID=7,length=159138663,assembly=GRCh37>",
    "contig.7": "<ID=8,length=146364022,assembly=GRCh37>",
    "contig.8": "<ID=9,length=141213431,assembly=GRCh37>",
    "contig.9": "<ID=10,length=135534747,assembly=GRCh37>",
    "contig.10": "<ID=11,length=135006516,assembly=GRCh37>",
    "contig.11": "<ID=12,length=133851895,assembly=GRCh37>",
    "contig.12": "<ID=13,length=115169878,assembly=GRCh37>",
    "contig.13": "<ID=14,length=107349540,assembly=GRCh37>",
    "contig.14": "<ID=15,length=102531392,assembly=GRCh37>",
    "contig.15": "<ID=16,length=90354753,assembly=GRCh37>",
    "contig.16": "<ID=17,length=81195210,assembly=GRCh37>",
    "contig.17": "<ID=18,length=78077248,assembly=GRCh37>",
    "contig.18": "<ID=19,length=59128983,assembly=GRCh37>",
    "contig.19": "<ID=20,length=63025520,assembly=GRCh37>",
    "contig.20": "<ID=21,length=48129895,assembly=GRCh37>",
    "contig.21": "<ID=22,length=51304566,assembly=GRCh37>",
    "contig.22": "<ID=X,length=155270560,assembly=GRCh37>",
    "contig.23": "<ID=Y,length=59373566,assembly=GRCh37>",
    "contig.24": "<ID=MT,length=16569,assembly=GRCh37>",
    "contig.25": "<ID=GL000207.1,length=4262,assembly=GRCh37>",
    "contig.26": "<ID=GL000226.1,length=15008,assembly=GRCh37>",
    "contig.27": "<ID=GL000229.1,length=19913,assembly=GRCh37>",
    "contig.28": "<ID=GL000231.1,length=27386,assembly=GRCh37>",
    "contig.29": "<ID=GL000210.1,length=27682,assembly=GRCh37>",
    "contig.30": "<ID=GL000239.1,length=33824,assembly=GRCh37>",
    "contig.31": "<ID=GL000235.1,length=34474,assembly=GRCh37>",
    "contig.32": "<ID=GL000201.1,length=36148,assembly=GRCh37>",
    "contig.33": "<ID=GL000247.1,length=36422,assembly=GRCh37>",
    "contig.34": "<ID=GL000245.1,length=36651,assembly=GRCh37>",
    "contig.35": "<ID=GL000197.1,length=37175,assembly=GRCh37>",
    "contig.36": "<ID=GL000203.1,length=37498,assembly=GRCh37>",
    "contig.37": "<ID=GL000246.1,length=38154,assembly=GRCh37>",
    "contig.38": "<ID=GL000249.1,length=38502,assembly=GRCh37>",
    "contig.39": "<ID=GL000196.1,length=38914,assembly=GRCh37>",
    "contig.40": "<ID=GL000248.1,length=39786,assembly=GRCh37>",
    "contig.41": "<ID=GL000244.1,length=39929,assembly=GRCh37>",
    "contig.42": "<ID=GL000238.1,length=39939,assembly=GRCh37>",
    "contig.43": "<ID=GL000202.1,length=40103,assembly=GRCh37>",
    "contig.44": "<ID=GL000234.1,length=40531,assembly=GRCh37>",
    "contig.45": "<ID=GL000232.1,length=40652,assembly=GRCh37>",
    "contig.46": "<ID=GL000206.1,length=41001,assembly=GRCh37>",
    "contig.47": "<ID=GL000240.1,length=41933,assembly=GRCh37>",
    "contig.48": "<ID=GL000236.1,length=41934,assembly=GRCh37>",
    "contig.49": "<ID=GL000241.1,length=42152,assembly=GRCh37>",
    "contig.50": "<ID=GL000243.1,length=43341,assembly=GRCh37>",
    "contig.51": "<ID=GL000242.1,length=43523,assembly=GRCh37>",
    "contig.52": "<ID=GL000230.1,length=43691,assembly=GRCh37>",
    "contig.53": "<ID=GL000237.1,length=45867,assembly=GRCh37>",
    "contig.54": "<ID=GL000233.1,length=45941,assembly=GRCh37>",
    "contig.55": "<ID=GL000204.1,length=81310,assembly=GRCh37>",
    "contig.56": "<ID=GL000198.1,length=90085,assembly=GRCh37>",
    "contig.57": "<ID=GL000208.1,length=92689,assembly=GRCh37>",
    "contig.58": "<ID=GL000191.1,length=106433,assembly=GRCh37>",
    "contig.59": "<ID=GL000227.1,length=128374,assembly=GRCh37>",
    "contig.60": "<ID=GL000228.1,length=129120,assembly=GRCh37>",
    "contig.61": "<ID=GL000214.1,length=137718,assembly=GRCh37>",
    "contig.62": "<ID=GL000221.1,length=155397,assembly=GRCh37>",
    "contig.63": "<ID=GL000209.1,length=159169,assembly=GRCh37>",
    "contig.64": "<ID=GL000218.1,length=161147,assembly=GRCh37>",
    "contig.65": "<ID=GL000220.1,length=161802,assembly=GRCh37>",
    "contig.66": "<ID=GL000213.1,length=164239,assembly=GRCh37>",
    "contig.67": "<ID=GL000211.1,length=166566,assembly=GRCh37>",
    "contig.68": "<ID=GL000199.1,length=169874,assembly=GRCh37>",
    "contig.69": "<ID=GL000217.1,length=172149,assembly=GRCh37>",
    "contig.70": "<ID=GL000216.1,length=172294,assembly=GRCh37>",
    "contig.71": "<ID=GL000215.1,length=172545,assembly=GRCh37>",
    "contig.72": "<ID=GL000205.1,length=174588,assembly=GRCh37>",
    "contig.73": "<ID=GL000219.1,length=179198,assembly=GRCh37>",
    "contig.74": "<ID=GL000224.1,length=179693,assembly=GRCh37>",
    "contig.75": "<ID=GL000223.1,length=180455,assembly=GRCh37>",
    "contig.76": "<ID=GL000195.1,length=182896,assembly=GRCh37>",
    "contig.77": "<ID=GL000212.1,length=186858,assembly=GRCh37>",
    "contig.78": "<ID=GL000222.1,length=186861,assembly=GRCh37>",
    "contig.79": "<ID=GL000200.1,length=187035,assembly=GRCh37>",
    "contig.80": "<ID=GL000193.1,length=189789,assembly=GRCh37>",
    "contig.81": "<ID=GL000194.1,length=191469,assembly=GRCh37>",
    "contig.82": "<ID=GL000225.1,length=211173,assembly=GRCh37>",
    "contig.83": "<ID=GL000192.1,length=547496,assembly=GRCh37>",
    "gwas_harmonisation_command": "--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/vcf_09_19b/bgzip_vcf/data.batch_1980.vcf.gz --id UKB-b:6519 --data /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/raw-output/data.batch_1980.txt.gz --cohort_cases 255812 --cohort_controls 194953 --ref /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/human_g1k_v37.fasta --json /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/ukb_gwas.json; 1.1.1",
    "file_date": "2019-09-13T07:55:12.398795",
    "bcftools_viewVersion": "1.9-74-g6af271c+htslib-1.9-64-g226b4a8",
    "bcftools_viewCommand": "view -T ^/mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-6519/mac_discard.txt -Oz /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-6519/UKB-b-6519_raw.vcf.gz; Date=Thu Oct 17 12:17:53 2019",
    "bcftools_viewCommand.1": "view -h /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukb-b-6519/ukb-b-6519.vcf.gz; Date=Sun May 10 14:49:33 2020"
}

LDSC

*********************************************************************
* LD Score Regression (LDSC)
* Version 1.0.1
* (C) 2014-2019 Brendan Bulik-Sullivan and Hilary Finucane
* Broad Institute of MIT and Harvard / MIT Department of Mathematics
* GNU General Public License v3
*********************************************************************
Call: 
./ldsc.py \
--h2 /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-6519/UKB-b-6519_data.vcf.gz \
--ref-ld-chr ../reference/eur_w_ld_chr/ \
--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-6519/ldsc.txt \
--w-ld-chr ../reference/eur_w_ld_chr/ 

Beginning analysis at Thu Oct 17 14:40:18 2019
Reading summary statistics from /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-6519/UKB-b-6519_data.vcf.gz ...
Read summary statistics for 9655477 SNPs.
Dropped 12917 SNPs with duplicated rs numbers.
Reading reference panel LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read reference panel LD Scores for 1290028 SNPs.
Removing partitioned LD Scores with zero variance.
Reading regression weight LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read regression weight LD Scores for 1290028 SNPs.
After merging with reference panel LD, 1288690 SNPs remain.
After merging with regression SNP LD, 1288690 SNPs remain.
Using two-step estimator with cutoff at 30.
Total Observed scale h2: 0.0736 (0.0033)
Lambda GC: 1.4983
Mean Chi^2: 1.7114
Intercept: 1.0235 (0.0083)
Ratio: 0.033 (0.0117)
Analysis finished at Thu Oct 17 14:42:05 2019
Total time elapsed: 1.0m:47.02s

QC metrics

Metrics

Metrics

{
    "af_correlation": 0.9495,
    "inflation_factor": 1.369,
    "mean_EFFECT": 7.1487e-06,
    "n": "-Inf",
    "n_snps": 9851866,
    "n_clumped_hits": 67,
    "n_p_sig": 11106,
    "n_mono": 0,
    "n_ns": 0,
    "n_mac": 0,
    "is_snpid_unique": true,
    "n_miss_EFFECT": 0,
    "n_miss_SE": 0,
    "n_miss_PVAL": 0,
    "n_miss_AF": 0,
    "n_miss_AF_reference": 155172,
    "n_est": "NA",
    "ratio_se_n": "NA",
    "mean_diff": "NaN",
    "ratio_diff": "NaN",
    "sd_y_est1": "NaN",
    "sd_y_est2": "NA",
    "r2_sum1": 0,
    "r2_sum2": 0,
    "r2_sum3": 0,
    "r2_sum4": 0,
    "ldsc_nsnp_merge_refpanel_ld": 1288690,
    "ldsc_nsnp_merge_regression_ld": 1288690,
    "ldsc_observed_scale_h2_beta": 0.0736,
    "ldsc_observed_scale_h2_se": 0.0033,
    "ldsc_intercept_beta": 1.0235,
    "ldsc_intercept_se": 0.0083,
    "ldsc_lambda_gc": 1.4983,
    "ldsc_mean_chisq": 1.7114,
    "ldsc_ratio": 0.033
}

Flags

name value
af_correlation FALSE
inflation_factor TRUE
n TRUE
is_snpid_non_unique FALSE
mean_EFFECT_nonfinite FALSE
mean_EFFECT_05 FALSE
mean_EFFECT_01 FALSE
mean_chisq TRUE
n_p_sig TRUE
miss_EFFECT FALSE
miss_SE FALSE
miss_PVAL FALSE
ldsc_ratio FALSE
ldsc_intercept_beta FALSE
n_clumped_hits FALSE
r2_sum1 FALSE
r2_sum2 FALSE
r2_sum3 FALSE
r2_sum4 FALSE

Definitions

General metrics

  • af_correlation: Correlation coefficient between AF and AF_reference.
  • inflation_factor (lambda): Genomic inflation factor.
  • mean_EFFECT: Mean of EFFECT size.
  • n: Maximum value of reported sample size across all SNPs, \(n\).
  • n_clumped_hits: Number of clumped hits.
  • n_snps: Number of SNPs
  • n_p_sig: Number of SNPs with pvalue below 5e-8.
  • n_mono: Number of monomorphic (MAF == 1 or MAF == 0) SNPs.
  • n_ns: Number of SNPs with nonsense values:
    • alleles other than A, C, G or T.
    • P-values < 0 or > 1.
    • negative or infinite standard errors (<= 0 or = Infinity).
    • infinite beta estimates or allele frequencies < 0 or > 1.
  • n_mac: Number of cases where MAC (\(2 \times N \times MAF\)) is less than 6.
  • is_snpid_unique: true if the combination of ID REF ALT is unique and therefore no duplication in snpid.
  • n_miss_<*>: Number of NA observations for <*> column.

se_n metrics

  • n_est: Estimated sample size value, \(\widehat{n}\).
  • ratio_se_n: \(\texttt{ratio_se_n} = \frac{\sqrt{\widehat{n}}}{\sqrt{n}}\). We expect ratio_se_n to be 1. When it is not 1, it implies that the trait did not have a variance of 1, the reported sample size is wrong, or that the SNP-level effective sample sizes differ markedly from the reported sample size.
  • mean_diff: \(\texttt{mean_diff} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta_j}{\texttt{n_snps}}\), mean difference between the standardised beta, predicted from P-values, and the observed beta. The difference should be very close to zero if trait has a variance of 1.
    • \(\widehat{\beta_j^{std}} = \sqrt{\frac{{z}_j^2 / ({z}_j^2 + n -2)}{2 \times {MAF}_j \times (1 - {MAF}_j)}} \times sign({z}_j)\),
    • \({z}_j = \frac{\beta_j}{{se}_j}\),
    • and \(\beta_j\) is the reported effect size.
  • ratio_diff: \(\texttt{ratio_diff} = |\frac{\texttt{mean_diff}}{\texttt{mean_diff2}}|\), absolute ratio between the mean of diff and the mean of diff2 (expected difference between the standardised beta predicted from P-values, and the standardised beta derived from the observed beta divided by the predicted SD; NOT reported). The ratio should be close to 1. If different from 1, then implies that the betas are not in a standard deviation scale.
    • \(\texttt{mean_diff2} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta^{\prime}_j}{\texttt{n_snps}}\)
    • \(\beta^{\prime}_j = \frac{\beta_j}{\widehat{\texttt{sd2}}_{y}}\)
  • sd_y_est1: The standard deviation for the trait inferred from the reported sample size, median standard errors for the SNP-trait assocations and SNP variances.
    • \(\widehat{\texttt{sd1}}_{y} = \frac{\sqrt{n} \times median({se}_j)}{C}\),
    • \(C = median(\frac{1}{\sqrt{2 \times {MAF}_j \times (1 - {MAF}_j)}})\),
    • and \({se}_j\) is the reported standard error.
  • sd_y_est2: The standard deviation for the trait inferred from the reported sample size, Z statistics for the SNP-trait effects (beta/se) and allele frequency.
    • \(\widehat{\texttt{sd2}}_{y} = median(\widehat{sd_j})\),
    • \(\widehat{sd_j} = \frac{\beta_j}{\widehat{\beta_j^{std}}}\),

r2 metrics

Sum of variance explained, calculated from the clumped top hits sample.

  • r2_sum<*>: r2 statistics under various assumptions
    • 1: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var1}}}\), \(\texttt{var1} = 1\).
    • 2: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var2}}}\), \(\texttt{var2} = {\widehat{\texttt{sd1}}_{y}}^2\),
    • 3: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var3}}}\), \(\texttt{var3} = {\widehat{\texttt{sd2}}_{y}}^2\),
    • 4: \(r^2 = \sum_j{\frac{F_j}{F_j + n - 2}}\), \(F = \frac{\beta_j^2}{{se}_j^2}\).

LDSC metrics

Metrics from LD regression

  • ldsc_nsnp_merge_refpanel_ld: Number of remaining SNPs after merging with reference panel LD.
  • ldsc_nsnp_merge_regression_ld: Number of remaining SNPs after merging with regression SNP LD.
  • ldsc_observed_scale_h2_{beta,se} Coefficient value and SE for total observed scale h2.
  • ldsc_intercept_{beta,se}: Coefficient value and SE for intercept. Intercept is expected to be 1.
  • ldsc_lambda_gc: Lambda GC statistics.
  • ldsc_mean_chisq: Mean \(\chi^2\) statistics.
  • ldsc_ratio: \(\frac{\texttt{ldsc_intercept_beta} - 1}{\texttt{ldsc_mean_chisq} - 1}\), the proportion of the inflation in the mean \(\chi^2\) that the LD Score regression intercepts ascribes to causes other than polygenic heritability. The value of ratio should be close to zero, though in practice values of 0.1-0.2 are not uncommon, probably due to sample/reference LD Score mismatch or model misspecification (e.g., low LD variants have slightly higher \(h^2\) per SNP).

Flags

When a metric needs attention, the flag should return TRUE.

  • af_correlation: abs(af_correlation) < 0.7.
  • inflation_factor: inflation_factor > 1.2.
  • n: n (max reported sample size) < 10000.
  • is_snpid_non_unique: NOT is_snpid_unique.
  • mean_EFFECT_nonfinite: mean(EFFECT) is NA, NaN, or Inf.
  • mean_EFFECT_05: abs(mean(EFFECT)) > 0.5.
  • mean_EFFECT_01: abs(mean(EFFECT)) > 0.1.
  • mean_chisq: ldsc_mean_chisq > 1.3 or ldsc_mean_chisq < 0.7.
  • n_p_sig: n_p_sig > 1000.
  • miss_<*>: n_miss_<*> / n_snps > 0.01.
  • ldsc_ratio: ldsc_ratio > 0.5
  • ldsc_intercept_beta: ldsc_intercept_beta > 1.5
  • n_clumped_hits: n_clumped_hits > 1000
  • r2_sum<*>: r2_sum<*> > 0.5

Plots

  • Manhattan plot
    • Red line: \(-log_{10}^{5 \times 10^{-8}}\)
    • Blue line: \(-log_{10}^{5 \times 10^{-5}}\)
  • QQ plot
  • AF plot
  • P-Z plot
  • beta_std plot: Scatter plot between \(\widehat{\beta_j^{std}}\) and \(\beta_j\)

Diagnostics

Details

Summary stats

skim_type skim_variable n_missing complete_rate character.min character.max character.empty character.n_unique character.whitespace logical.mean logical.count numeric.mean numeric.sd numeric.p0 numeric.p25 numeric.p50 numeric.p75 numeric.p100 numeric.hist
character ID 0 1.0000000 3 58 0 9642622 0 NA NA NA NA NA NA NA NA NA NA
character REF 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
character ALT 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
logical N 9655477 0.0000000 NA NA NA NA NA NaN : NA NA NA NA NA NA NA NA
numeric CHROM 0 1.0000000 NA NA NA NA NA NA NA 8.626432e+00 5.750394e+00 1.0000000 4.000000e+00 8.000000e+00 1.300000e+01 2.200000e+01 ▇▅▃▂▂
numeric POS 0 1.0000000 NA NA NA NA NA NA NA 7.883991e+07 5.629337e+07 828.0000000 3.256179e+07 6.944337e+07 1.145682e+08 2.492385e+08 ▇▆▅▂▁
numeric EFFECT 0 1.0000000 NA NA NA NA NA NA NA 7.100000e-06 4.642200e-03 -0.0724386 -1.683400e-03 9.200000e-06 1.709700e-03 9.248790e-02 ▁▁▇▁▁
numeric SE 0 1.0000000 NA NA NA NA NA NA NA 3.293900e-03 2.906800e-03 0.0009894 1.203200e-03 1.973900e-03 4.442200e-03 5.201850e-02 ▇▁▁▁▁
numeric PVAL 0 1.0000000 NA NA NA NA NA NA NA 4.513227e-01 3.019329e-01 0.0000000 1.800002e-01 4.299995e-01 7.099994e-01 1.000000e+00 ▇▆▆▅▅
numeric PVAL_ztest 0 1.0000000 NA NA NA NA NA NA NA 4.513231e-01 3.019085e-01 0.0000000 1.772580e-01 4.341920e-01 7.130746e-01 9.999999e-01 ▇▆▅▅▅
numeric AF 0 1.0000000 NA NA NA NA NA NA NA 2.068948e-01 2.570372e-01 0.0013690 1.457600e-02 8.282200e-02 3.230560e-01 9.986310e-01 ▇▂▁▁▁
numeric AF_reference 155172 0.9839291 NA NA NA NA NA NA NA 2.092550e-01 2.486658e-01 0.0000000 1.257990e-02 1.034350e-01 3.252800e-01 1.000000e+00 ▇▂▁▁▁

Head and tail

CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
1 49298 rs200943160 T C -0.0000853 0.0018204 0.9599999 0.9626118 0.623766 0.7821490 NA
1 54676 rs2462492 C T -0.0024096 0.0018027 0.1800002 0.1813361 0.400392 NA NA
1 86028 rs114608975 T C 0.0012307 0.0028817 0.6700003 0.6693364 0.103586 0.0277556 NA
1 91536 rs6702460 G T -0.0025106 0.0017748 0.1600000 0.1572028 0.456799 0.4207270 NA
1 234313 rs8179466 C T 0.0003614 0.0034988 0.9199999 0.9177199 0.074511 NA NA
1 534192 rs6680723 C T 0.0020086 0.0020277 0.3200000 0.3218713 0.240926 NA NA
1 546697 rs12025928 A G 0.0036165 0.0025281 0.1499999 0.1525622 0.913424 NA NA
1 693731 rs12238997 A G 0.0035971 0.0016988 0.0340001 0.0342244 0.116334 0.1417730 NA
1 705882 rs72631875 G A -0.0019889 0.0024902 0.4199997 0.4244571 0.067269 0.0315495 NA
1 706368 rs55727773 A G -0.0036470 0.0012585 0.0038000 0.0037559 0.515671 0.2751600 NA
CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
22 51219704 rs147475742 G A 0.0045933 0.0026445 0.0819993 0.0824029 0.041918 0.0473243 NA
22 51219766 rs182321900 C T 0.0060886 0.0123788 0.6200004 0.6228215 0.001919 NA NA
22 51220146 rs868950473 C T 0.0092713 0.0122609 0.4500005 0.4495485 0.001968 NA NA
22 51221190 rs369304721 G A 0.0048105 0.0026394 0.0680002 0.0683698 0.049715 NA NA
22 51221731 rs115055839 T C 0.0028605 0.0019742 0.1499999 0.1473497 0.073207 0.0625000 NA
22 51222100 rs114553188 G T -0.0002243 0.0023258 0.9199999 0.9231848 0.054370 0.0880591 NA
22 51223637 rs375798137 G A -0.0005864 0.0023371 0.8000000 0.8018940 0.054000 0.0788738 NA
22 51229805 rs9616985 T C 0.0029058 0.0019814 0.1400000 0.1424940 0.073038 0.0730831 NA
22 51232488 rs376461333 A G -0.0004158 0.0046647 0.9299999 0.9289693 0.020038 NA NA
22 51237063 rs3896457 T C -0.0005775 0.0012118 0.6300007 0.6336816 0.298000 0.2050720 NA

bcf preview

1   49298   rs10399793  T   C   .   PASS    AF=0.623766 ES:SE:LP:AF:ID  -8.53333e-05:0.00182039:0.0177288:0.623766:rs10399793
1   54676   rs2462492   C   T   .   PASS    AF=0.400392 ES:SE:LP:AF:ID  -0.00240957:0.00180269:0.744727:0.400392:rs2462492
1   86028   rs114608975 T   C   .   PASS    AF=0.103586 ES:SE:LP:AF:ID  0.00123066:0.00288171:0.173925:0.103586:rs114608975
1   91536   rs6702460   G   T   .   PASS    AF=0.456799 ES:SE:LP:AF:ID  -0.0025106:0.00177485:0.79588:0.456799:rs6702460
1   234313  rs8179466   C   T   .   PASS    AF=0.074511 ES:SE:LP:AF:ID  0.000361444:0.00349876:0.0362122:0.074511:rs8179466
1   534192  rs6680723   C   T   .   PASS    AF=0.240926 ES:SE:LP:AF:ID  0.00200865:0.00202767:0.49485:0.240926:rs6680723
1   546697  rs12025928  A   G   .   PASS    AF=0.913424 ES:SE:LP:AF:ID  0.00361649:0.00252806:0.823909:0.913424:rs12025928
1   693731  rs12238997  A   G   .   PASS    AF=0.116334 ES:SE:LP:AF:ID  0.00359709:0.00169881:1.46852:0.116334:rs12238997
1   705882  rs72631875  G   A   .   PASS    AF=0.067269 ES:SE:LP:AF:ID  -0.00198893:0.00249017:0.376751:0.067269:rs72631875
1   706368  rs12029736  A   G   .   PASS    AF=0.515671 ES:SE:LP:AF:ID  -0.00364703:0.00125848:2.42022:0.515671:rs12029736
1   714596  rs149887893 T   C   .   PASS    AF=0.033018 ES:SE:LP:AF:ID  0.00143083:0.00317257:0.187087:0.033018:rs149887893
1   715265  rs12184267  C   T   .   PASS    AF=0.036638 ES:SE:LP:AF:ID  0.00170808:0.00288163:0.259637:0.036638:rs12184267
1   715367  rs12184277  A   G   .   PASS    AF=0.036756 ES:SE:LP:AF:ID  0.00168068:0.00287061:0.251812:0.036756:rs12184277
1   717485  rs12184279  C   A   .   PASS    AF=0.036455 ES:SE:LP:AF:ID  0.00221624:0.00289133:0.356547:0.036455:rs12184279
1   717587  rs144155419 G   A   .   PASS    AF=0.016422 ES:SE:LP:AF:ID  -0.00309042:0.00445022:0.309804:0.016422:rs144155419
1   720381  rs116801199 G   T   .   PASS    AF=0.036997 ES:SE:LP:AF:ID  0.00200643:0.0028592:0.318759:0.036997:rs116801199
1   721290  rs12565286  G   C   .   PASS    AF=0.037091 ES:SE:LP:AF:ID  0.00189536:0.0028495:0.29243:0.037091:rs12565286
1   722670  rs116030099 T   C   .   PASS    AF=0.101231 ES:SE:LP:AF:ID  0.00107847:0.0020762:0.221849:0.101231:rs116030099
1   723891  rs2977670   G   C   .   PASS    AF=0.959081 ES:SE:LP:AF:ID  -0.00154085:0.00274819:0.236572:0.959081:rs2977670
1   724849  rs12126395  C   A   .   PASS    AF=0.031476 ES:SE:LP:AF:ID  0.0049917:0.00498525:0.49485:0.031476:rs12126395
1   725060  rs865924913 A   T   .   PASS    AF=0.05329  ES:SE:LP:AF:ID  -0.00141744:0.00396708:0.142668:0.05329:rs865924913
1   726794  rs28454925  C   G   .   PASS    AF=0.036614 ES:SE:LP:AF:ID  0.00228776:0.00286767:0.366532:0.036614:rs28454925
1   729632  rs116720794 C   T   .   PASS    AF=0.036928 ES:SE:LP:AF:ID  0.00227389:0.00284166:0.376751:0.036928:rs116720794
1   729679  rs4951859   C   G   .   PASS    AF=0.843189 ES:SE:LP:AF:ID  -0.00326142:0.00147237:1.56864:0.843189:rs4951859
1   730087  rs148120343 T   C   .   PASS    AF=0.055905 ES:SE:LP:AF:ID  0.00744888:0.00238468:2.74473:0.055905:rs148120343
1   731718  rs58276399  T   C   .   PASS    AF=0.122309 ES:SE:LP:AF:ID  0.00379514:0.00161167:1.72125:0.122309:rs58276399
1   732989  rs369030935 C   T   .   PASS    AF=0.025724 ES:SE:LP:AF:ID  -0.00237324:0.00396334:0.259637:0.025724:rs369030935
1   734349  rs141242758 T   C   .   PASS    AF=0.121546 ES:SE:LP:AF:ID  0.00383387:0.00161238:1.76955:0.121546:rs141242758
1   736289  rs79010578  T   A   .   PASS    AF=0.132354 ES:SE:LP:AF:ID  0.00386651:0.00158898:1.82391:0.132354:rs79010578
1   736689  rs181876450 T   C   .   PASS    AF=0.011127 ES:SE:LP:AF:ID  0.00228293:0.0057807:0.161151:0.011127:rs181876450
1   740284  rs61770167  C   T   .   PASS    AF=0.005709 ES:SE:LP:AF:ID  -5.71065e-05:0.00745046:0.00436481:0.005709:rs61770167
1   742813  rs112573343 C   T   .   PASS    AF=0.00226  ES:SE:LP:AF:ID  0.00766141:0.0125639:0.267606:0.00226:rs112573343
1   752478  rs146277091 G   A   .   PASS    AF=0.036845 ES:SE:LP:AF:ID  0.00261797:0.00281294:0.455932:0.036845:rs146277091
1   752566  rs3094315   G   A   .   PASS    AF=0.838906 ES:SE:LP:AF:ID  -0.00343563:0.00142597:1.79588:0.838906:rs3094315
1   752721  rs3131972   A   G   .   PASS    AF=0.838534 ES:SE:LP:AF:ID  -0.00338914:0.00142442:1.76955:0.838534:rs3131972
1   753405  rs3115860   C   A   .   PASS    AF=0.86978  ES:SE:LP:AF:ID  -0.00353063:0.00152868:1.67778:0.86978:rs3115860
1   753541  rs2073813   G   A   .   PASS    AF=0.129874 ES:SE:LP:AF:ID  0.00357432:0.00153177:1.69897:0.129874:rs2073813
1   754063  rs12184312  G   T   .   PASS    AF=0.037351 ES:SE:LP:AF:ID  0.00206222:0.00276543:0.337242:0.037351:rs12184312
1   754105  rs12184325  C   T   .   PASS    AF=0.037597 ES:SE:LP:AF:ID  0.0022003:0.00274789:0.376751:0.037597:rs12184325
1   754182  rs3131969   A   G   .   PASS    AF=0.869122 ES:SE:LP:AF:ID  -0.0035576:0.00152568:1.69897:0.869122:rs3131969
1   754192  rs3131968   A   G   .   PASS    AF=0.869221 ES:SE:LP:AF:ID  -0.00358219:0.00152629:1.72125:0.869221:rs3131968
1   754211  rs12184313  G   A   .   PASS    AF=0.037553 ES:SE:LP:AF:ID  0.00201599:0.00275984:0.327902:0.037553:rs12184313
1   754334  rs3131967   T   C   .   PASS    AF=0.869125 ES:SE:LP:AF:ID  -0.00357785:0.00152565:1.72125:0.869125:rs3131967
1   754433  rs150578204 G   A   .   PASS    AF=0.005132 ES:SE:LP:AF:ID  -0.00658854:0.00782453:0.39794:0.005132:rs150578204
1   754458  rs142682604 G   T   .   PASS    AF=0.005097 ES:SE:LP:AF:ID  -0.00649364:0.00784511:0.387216:0.005097:rs142682604
1   754503  rs3115859   G   A   .   PASS    AF=0.837995 ES:SE:LP:AF:ID  -0.00320533:0.00142052:1.61979:0.837995:rs3115859
1   754629  rs10454459  A   G   .   PASS    AF=0.037566 ES:SE:LP:AF:ID  0.00171126:0.00276378:0.267606:0.037566:rs10454459
1   754964  rs3131966   C   T   .   PASS    AF=0.838626 ES:SE:LP:AF:ID  -0.00315626:0.00142453:1.56864:0.838626:rs3131966
1   755240  rs181660517 T   G   .   PASS    AF=0.013774 ES:SE:LP:AF:ID  -0.000261817:0.00497272:0.0177288:0.013774:rs181660517
1   755435  rs184270342 T   G   .   PASS    AF=0.005536 ES:SE:LP:AF:ID  0.00251214:0.00767833:0.130768:0.005536:rs184270342