Summary

Summary {data-width=650}

Manhattan plot

manhattan_plot

manhattan_plot

QQ plot

qq_plot

qq_plot

AF plot

af_plot

af_plot

P-Z plot

pz_plot

pz_plot

beta_std plot

beta_std_plot

beta_std_plot

Metadata

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    "gwas_harmonisation_command": "--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/vcf_09_19b/bgzip_vcf/data.batch_3082.vcf.gz --id UKB-b:18389 --data /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/raw-output/data.batch_3082.txt.gz --cohort_controls 306379 --ref /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/human_g1k_v37.fasta --json /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/ukb_gwas.json; 1.1.1",
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}
 

LDSC

*********************************************************************
* LD Score Regression (LDSC)
* Version 1.0.1
* (C) 2014-2019 Brendan Bulik-Sullivan and Hilary Finucane
* Broad Institute of MIT and Harvard / MIT Department of Mathematics
* GNU General Public License v3
*********************************************************************
Call: 
./ldsc.py \
--h2 /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-18389/UKB-b-18389_data.vcf.gz \
--ref-ld-chr ../reference/eur_w_ld_chr/ \
--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-18389/ldsc.txt \
--w-ld-chr ../reference/eur_w_ld_chr/ 

Beginning analysis at Thu Oct 17 14:40:18 2019
Reading summary statistics from /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-18389/UKB-b-18389_data.vcf.gz ...
Read summary statistics for 9762976 SNPs.
Dropped 13904 SNPs with duplicated rs numbers.
Reading reference panel LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read reference panel LD Scores for 1290028 SNPs.
Removing partitioned LD Scores with zero variance.
Reading regression weight LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read regression weight LD Scores for 1290028 SNPs.
After merging with reference panel LD, 1288935 SNPs remain.
After merging with regression SNP LD, 1288935 SNPs remain.
Using two-step estimator with cutoff at 30.
Total Observed scale h2: -0.0011 (0.0015)
Lambda GC: 1.0016
Mean Chi^2: 1.0048
Intercept: 1.0115 (0.006)
Ratio: 2.3958 (1.252)
Analysis finished at Thu Oct 17 14:42:05 2019
Total time elapsed: 1.0m:47.09s

QC metrics

Metrics

Metrics

{
    "af_correlation": 0.9496,
    "inflation_factor": 1,
    "mean_EFFECT": 6.6526e-06,
    "n": "-Inf",
    "n_snps": 9851866,
    "n_clumped_hits": 0,
    "n_p_sig": 147,
    "n_mono": 0,
    "n_ns": 0,
    "n_mac": 0,
    "is_snpid_unique": true,
    "n_miss_EFFECT": 0,
    "n_miss_SE": 0,
    "n_miss_PVAL": 0,
    "n_miss_AF": 0,
    "n_miss_AF_reference": 174035,
    "n_est": "NA",
    "ratio_se_n": "NA",
    "mean_diff": "NaN",
    "ratio_diff": "NaN",
    "sd_y_est1": "NaN",
    "sd_y_est2": "NA",
    "r2_sum1": 0,
    "r2_sum2": 0,
    "r2_sum3": 0,
    "r2_sum4": 0,
    "ldsc_nsnp_merge_refpanel_ld": 1288935,
    "ldsc_nsnp_merge_regression_ld": 1288935,
    "ldsc_observed_scale_h2_beta": "NA",
    "ldsc_observed_scale_h2_se": "NA",
    "ldsc_intercept_beta": 1.0115,
    "ldsc_intercept_se": 0.006,
    "ldsc_lambda_gc": 1.0016,
    "ldsc_mean_chisq": 1.0048,
    "ldsc_ratio": 2.3958
}
 

Flags

name value
af_correlation FALSE
inflation_factor FALSE
n TRUE
is_snpid_non_unique FALSE
mean_EFFECT_nonfinite FALSE
mean_EFFECT_05 FALSE
mean_EFFECT_01 FALSE
mean_chisq FALSE
n_p_sig FALSE
miss_EFFECT FALSE
miss_SE FALSE
miss_PVAL FALSE
ldsc_ratio TRUE
ldsc_intercept_beta FALSE
n_clumped_hits FALSE
r2_sum1 FALSE
r2_sum2 FALSE
r2_sum3 FALSE
r2_sum4 FALSE

Definitions

General metrics

  • af_correlation: Correlation coefficient between AF and AF_reference.
  • inflation_factor (lambda): Genomic inflation factor.
  • mean_EFFECT: Mean of EFFECT size.
  • n: Maximum value of reported sample size across all SNPs, \(n\).
  • n_clumped_hits: Number of clumped hits.
  • n_snps: Number of SNPs
  • n_p_sig: Number of SNPs with pvalue below 5e-8.
  • n_mono: Number of monomorphic (MAF == 1 or MAF == 0) SNPs.
  • n_ns: Number of SNPs with nonsense values:
    • alleles other than A, C, G or T.
    • P-values < 0 or > 1.
    • negative or infinite standard errors (<= 0 or = Infinity).
    • infinite beta estimates or allele frequencies < 0 or > 1.
  • n_mac: Number of cases where MAC (\(2 \times N \times MAF\)) is less than 6.
  • is_snpid_unique: true if the combination of ID REF ALT is unique and therefore no duplication in snpid.
  • n_miss_<*>: Number of NA observations for <*> column.

se_n metrics

  • n_est: Estimated sample size value, \(\widehat{n}\).
  • ratio_se_n: \(\texttt{ratio_se_n} = \frac{\sqrt{\widehat{n}}}{\sqrt{n}}\). We expect ratio_se_n to be 1. When it is not 1, it implies that the trait did not have a variance of 1, the reported sample size is wrong, or that the SNP-level effective sample sizes differ markedly from the reported sample size.
  • mean_diff: \(\texttt{mean_diff} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta_j}{\texttt{n_snps}}\), mean difference between the standardised beta, predicted from P-values, and the observed beta. The difference should be very close to zero if trait has a variance of 1.
    • \(\widehat{\beta_j^{std}} = \sqrt{\frac{{z}_j^2 / ({z}_j^2 + n -2)}{2 \times {MAF}_j \times (1 - {MAF}_j)}} \times sign({z}_j)\),
    • \({z}_j = \frac{\beta_j}{{se}_j}\),
    • and \(\beta_j\) is the reported effect size.
  • ratio_diff: \(\texttt{ratio_diff} = |\frac{\texttt{mean_diff}}{\texttt{mean_diff2}}|\), absolute ratio between the mean of diff and the mean of diff2 (expected difference between the standardised beta predicted from P-values, and the standardised beta derived from the observed beta divided by the predicted SD; NOT reported). The ratio should be close to 1. If different from 1, then implies that the betas are not in a standard deviation scale.
    • \(\texttt{mean_diff2} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta^{\prime}_j}{\texttt{n_snps}}\)
    • \(\beta^{\prime}_j = \frac{\beta_j}{\widehat{\texttt{sd2}}_{y}}\)
  • sd_y_est1: The standard deviation for the trait inferred from the reported sample size, median standard errors for the SNP-trait assocations and SNP variances.
    • \(\widehat{\texttt{sd1}}_{y} = \frac{\sqrt{n} \times median({se}_j)}{C}\),
    • \(C = median(\frac{1}{\sqrt{2 \times {MAF}_j \times (1 - {MAF}_j)}})\),
    • and \({se}_j\) is the reported standard error.
  • sd_y_est2: The standard deviation for the trait inferred from the reported sample size, Z statistics for the SNP-trait effects (beta/se) and allele frequency.
    • \(\widehat{\texttt{sd2}}_{y} = median(\widehat{sd_j})\),
    • \(\widehat{sd_j} = \frac{\beta_j}{\widehat{\beta_j^{std}}}\),

r2 metrics

Sum of variance explained, calculated from the clumped top hits sample.

  • r2_sum<*>: r2 statistics under various assumptions
    • 1: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var1}}}\), \(\texttt{var1} = 1\).
    • 2: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var2}}}\), \(\texttt{var2} = {\widehat{\texttt{sd1}}_{y}}^2\),
    • 3: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var3}}}\), \(\texttt{var3} = {\widehat{\texttt{sd2}}_{y}}^2\),
    • 4: \(r^2 = \sum_j{\frac{F_j}{F_j + n - 2}}\), \(F = \frac{\beta_j^2}{{se}_j^2}\).

LDSC metrics

Metrics from LD regression

  • ldsc_nsnp_merge_refpanel_ld: Number of remaining SNPs after merging with reference panel LD.
  • ldsc_nsnp_merge_regression_ld: Number of remaining SNPs after merging with regression SNP LD.
  • ldsc_observed_scale_h2_{beta,se} Coefficient value and SE for total observed scale h2.
  • ldsc_intercept_{beta,se}: Coefficient value and SE for intercept. Intercept is expected to be 1.
  • ldsc_lambda_gc: Lambda GC statistics.
  • ldsc_mean_chisq: Mean \(\chi^2\) statistics.
  • ldsc_ratio: \(\frac{\texttt{ldsc_intercept_beta} - 1}{\texttt{ldsc_mean_chisq} - 1}\), the proportion of the inflation in the mean \(\chi^2\) that the LD Score regression intercepts ascribes to causes other than polygenic heritability. The value of ratio should be close to zero, though in practice values of 0.1-0.2 are not uncommon, probably due to sample/reference LD Score mismatch or model misspecification (e.g., low LD variants have slightly higher \(h^2\) per SNP).

Flags

When a metric needs attention, the flag should return TRUE.

  • af_correlation: abs(af_correlation) < 0.7.
  • inflation_factor: inflation_factor > 1.2.
  • n: n (max reported sample size) < 10000.
  • is_snpid_non_unique: NOT is_snpid_unique.
  • mean_EFFECT_nonfinite: mean(EFFECT) is NA, NaN, or Inf.
  • mean_EFFECT_05: abs(mean(EFFECT)) > 0.5.
  • mean_EFFECT_01: abs(mean(EFFECT)) > 0.1.
  • mean_chisq: ldsc_mean_chisq > 1.3 or ldsc_mean_chisq < 0.7.
  • n_p_sig: n_p_sig > 1000.
  • miss_<*>: n_miss_<*> / n_snps > 0.01.
  • ldsc_ratio: ldsc_ratio > 0.5
  • ldsc_intercept_beta: ldsc_intercept_beta > 1.5
  • n_clumped_hits: n_clumped_hits > 1000
  • r2_sum<*>: r2_sum<*> > 0.5

Plots

  • Manhattan plot
    • Red line: \(-log_{10}^{5 \times 10^{-8}}\)
    • Blue line: \(-log_{10}^{5 \times 10^{-5}}\)
  • QQ plot
  • AF plot
  • P-Z plot
  • beta_std plot: Scatter plot between \(\widehat{\beta_j^{std}}\) and \(\beta_j\)

Diagnostics

Details

Summary stats

skim_type skim_variable n_missing complete_rate character.min character.max character.empty character.n_unique character.whitespace logical.mean logical.count numeric.mean numeric.sd numeric.p0 numeric.p25 numeric.p50 numeric.p75 numeric.p100 numeric.hist
character ID 0 1.000000 3 58 0 9749137 0 NA NA NA NA NA NA NA NA NA NA
character REF 0 1.000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
character ALT 0 1.000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
logical N 9762976 0.000000 NA NA NA NA NA NaN : NA NA NA NA NA NA NA NA
numeric CHROM 0 1.000000 NA NA NA NA NA NA NA 8.623161e+00 5.748991e+00 1.0000000 4.000000e+00 8.000000e+00 1.300000e+01 2.200000e+01 ▇▅▃▂▂
numeric POS 0 1.000000 NA NA NA NA NA NA NA 7.886049e+07 5.629120e+07 828.0000000 3.258219e+07 6.948158e+07 1.145965e+08 2.492385e+08 ▇▆▅▂▁
numeric EFFECT 0 1.000000 NA NA NA NA NA NA NA 6.700000e-06 6.834000e-04 -0.0135718 -2.174000e-04 -7.500000e-06 1.970000e-04 1.776490e-02 ▁▁▇▁▁
numeric SE 0 1.000000 NA NA NA NA NA NA NA 4.941000e-04 4.538000e-04 0.0001427 1.742000e-04 2.892000e-04 6.601000e-04 7.437500e-03 ▇▁▁▁▁
numeric PVAL 0 1.000000 NA NA NA NA NA NA NA 4.983082e-01 2.883279e-01 0.0000000 2.500000e-01 5.000000e-01 7.499995e-01 1.000000e+00 ▇▇▇▇▇
numeric PVAL_ztest 0 1.000000 NA NA NA NA NA NA NA 4.983077e-01 2.883027e-01 0.0000000 2.490303e-01 4.967301e-01 7.481675e-01 1.000000e+00 ▇▇▇▇▇
numeric AF 0 1.000000 NA NA NA NA NA NA NA 2.049607e-01 2.568895e-01 0.0011430 1.377600e-02 8.008000e-02 3.192550e-01 9.988570e-01 ▇▂▁▁▁
numeric AF_reference 174035 0.982174 NA NA NA NA NA NA NA 2.078656e-01 2.484510e-01 0.0000000 1.218050e-02 1.014380e-01 3.224840e-01 1.000000e+00 ▇▂▁▁▁

Head and tail

CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
1 49298 rs200943160 T C 0.0000691 0.0002623 0.7899998 0.7923303 0.623780 0.7821490 NA
1 54676 rs2462492 C T 0.0003005 0.0002596 0.2500000 0.2469307 0.400989 NA NA
1 86028 rs114608975 T C -0.0001430 0.0004157 0.7300002 0.7307955 0.103433 0.0277556 NA
1 91536 rs6702460 G T 0.0004535 0.0002555 0.0759994 0.0759292 0.457148 0.4207270 NA
1 234313 rs8179466 C T 0.0006361 0.0005037 0.2099999 0.2065937 0.074491 NA NA
1 534192 rs6680723 C T 0.0002804 0.0002917 0.3400001 0.3365458 0.240867 NA NA
1 546697 rs12025928 A G -0.0000787 0.0003650 0.8300000 0.8292208 0.913709 NA NA
1 693731 rs12238997 A G -0.0000686 0.0002451 0.7800007 0.7796520 0.115979 0.1417730 NA
1 705882 rs72631875 G A 0.0000888 0.0003597 0.8000000 0.8049447 0.067096 0.0315495 NA
1 706368 rs55727773 A G -0.0001100 0.0001816 0.5400003 0.5447390 0.516008 0.2751600 NA
CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
22 51219704 rs147475742 G A 0.0004618 0.0003808 0.2300001 0.2253271 0.042073 0.0473243 NA
22 51219766 rs182321900 C T 0.0014496 0.0017720 0.4100001 0.4133360 0.001942 NA NA
22 51220146 rs868950473 C T 0.0013239 0.0017616 0.4500005 0.4523330 0.001979 NA NA
22 51221190 rs369304721 G A 0.0004073 0.0003795 0.2800000 0.2831657 0.049993 NA NA
22 51221731 rs115055839 T C 0.0000834 0.0002838 0.7700005 0.7687550 0.073701 0.0625000 NA
22 51222100 rs114553188 G T 0.0000814 0.0003349 0.8100000 0.8079754 0.054491 0.0880591 NA
22 51223637 rs375798137 G A 0.0000925 0.0003365 0.7800007 0.7833979 0.054130 0.0788738 NA
22 51229805 rs9616985 T C 0.0000873 0.0002848 0.7600007 0.7591754 0.073526 0.0730831 NA
22 51232488 rs376461333 A G 0.0009274 0.0006727 0.1700000 0.1680045 0.019989 NA NA
22 51237063 rs3896457 T C -0.0001113 0.0001747 0.5199996 0.5242123 0.298049 0.2050720 NA

bcf preview

1   49298   rs10399793  T   C   .   PASS    AF=0.62378  ES:SE:LP:AF:ID  6.90675e-05:0.000262329:0.102373:0.62378:rs10399793
1   54676   rs2462492   C   T   .   PASS    AF=0.400989 ES:SE:LP:AF:ID  0.000300542:0.000259572:0.60206:0.400989:rs2462492
1   86028   rs114608975 T   C   .   PASS    AF=0.103433 ES:SE:LP:AF:ID  -0.000143044:0.000415744:0.136677:0.103433:rs114608975
1   91536   rs6702460   G   T   .   PASS    AF=0.457148 ES:SE:LP:AF:ID  0.000453473:0.000255505:1.11919:0.457148:rs6702460
1   234313  rs8179466   C   T   .   PASS    AF=0.074491 ES:SE:LP:AF:ID  0.000636115:0.000503659:0.677781:0.074491:rs8179466
1   534192  rs6680723   C   T   .   PASS    AF=0.240867 ES:SE:LP:AF:ID  0.000280362:0.000291736:0.468521:0.240867:rs6680723
1   546697  rs12025928  A   G   .   PASS    AF=0.913709 ES:SE:LP:AF:ID  -7.8741e-05:0.000365047:0.0809219:0.913709:rs12025928
1   693731  rs12238997  A   G   .   PASS    AF=0.115979 ES:SE:LP:AF:ID  -6.85826e-05:0.000245137:0.107905:0.115979:rs12238997
1   705882  rs72631875  G   A   .   PASS    AF=0.067096 ES:SE:LP:AF:ID  8.8823e-05:0.000359676:0.09691:0.067096:rs72631875
1   706368  rs12029736  A   G   .   PASS    AF=0.516008 ES:SE:LP:AF:ID  -0.000110001:0.000181621:0.267606:0.516008:rs12029736
1   714596  rs149887893 T   C   .   PASS    AF=0.032723 ES:SE:LP:AF:ID  0.000181981:0.000459965:0.161151:0.032723:rs149887893
1   715265  rs12184267  C   T   .   PASS    AF=0.036302 ES:SE:LP:AF:ID  0.000156645:0.000417803:0.148742:0.036302:rs12184267
1   715367  rs12184277  A   G   .   PASS    AF=0.036412 ES:SE:LP:AF:ID  4.43358e-05:0.000416245:0.0362122:0.036412:rs12184277
1   717485  rs12184279  C   A   .   PASS    AF=0.036134 ES:SE:LP:AF:ID  0.000213295:0.000419148:0.21467:0.036134:rs12184279
1   717587  rs144155419 G   A   .   PASS    AF=0.016397 ES:SE:LP:AF:ID  -0.000866541:0.000641861:0.744727:0.016397:rs144155419
1   720381  rs116801199 G   T   .   PASS    AF=0.036652 ES:SE:LP:AF:ID  0.000201275:0.000414597:0.200659:0.036652:rs116801199
1   721290  rs12565286  G   C   .   PASS    AF=0.036746 ES:SE:LP:AF:ID  0.000198895:0.000413152:0.200659:0.036746:rs12565286
1   722670  rs116030099 T   C   .   PASS    AF=0.101078 ES:SE:LP:AF:ID  0.000210745:0.000299866:0.318759:0.101078:rs116030099
1   723891  rs2977670   G   C   .   PASS    AF=0.959485 ES:SE:LP:AF:ID  -0.000362095:0.000398395:0.443698:0.959485:rs2977670
1   724849  rs12126395  C   A   .   PASS    AF=0.031332 ES:SE:LP:AF:ID  -0.00108151:0.000720364:0.886057:0.031332:rs12126395
1   725060  rs865924913 A   T   .   PASS    AF=0.05357  ES:SE:LP:AF:ID  -9.2317e-05:0.00056902:0.0604807:0.05357:rs865924913
1   726794  rs28454925  C   G   .   PASS    AF=0.036286 ES:SE:LP:AF:ID  0.000160275:0.000415735:0.154902:0.036286:rs28454925
1   729632  rs116720794 C   T   .   PASS    AF=0.036598 ES:SE:LP:AF:ID  8.71203e-05:0.000411899:0.0809219:0.036598:rs116720794
1   729679  rs4951859   C   G   .   PASS    AF=0.843889 ES:SE:LP:AF:ID  -1.10085e-06:0.000212609:-0:0.843889:rs4951859
1   730087  rs148120343 T   C   .   PASS    AF=0.055808 ES:SE:LP:AF:ID  -2.44646e-05:0.000343706:0.0268721:0.055808:rs148120343
1   731718  rs58276399  T   C   .   PASS    AF=0.121977 ES:SE:LP:AF:ID  -5.86839e-05:0.000232484:0.09691:0.121977:rs58276399
1   732989  rs369030935 C   T   .   PASS    AF=0.025679 ES:SE:LP:AF:ID  -0.000286939:0.00057215:0.207608:0.025679:rs369030935
1   734349  rs141242758 T   C   .   PASS    AF=0.121233 ES:SE:LP:AF:ID  -5.08137e-05:0.000232571:0.0809219:0.121233:rs141242758
1   736289  rs79010578  T   A   .   PASS    AF=0.131792 ES:SE:LP:AF:ID  7.31038e-05:0.000229438:0.124939:0.131792:rs79010578
1   736689  rs181876450 T   C   .   PASS    AF=0.011089 ES:SE:LP:AF:ID  -0.000971411:0.000835196:0.619789:0.011089:rs181876450
1   740284  rs61770167  C   T   .   PASS    AF=0.005633 ES:SE:LP:AF:ID  0.000491051:0.00108324:0.187087:0.005633:rs61770167
1   742813  rs112573343 C   T   .   PASS    AF=0.002275 ES:SE:LP:AF:ID  -0.000697615:0.00179726:0.154902:0.002275:rs112573343
1   752478  rs146277091 G   A   .   PASS    AF=0.036492 ES:SE:LP:AF:ID  0.000171409:0.000407807:0.173925:0.036492:rs146277091
1   752566  rs3094315   G   A   .   PASS    AF=0.83964  ES:SE:LP:AF:ID  3.56379e-05:0.000205921:0.0655015:0.83964:rs3094315
1   752721  rs3131972   A   G   .   PASS    AF=0.839274 ES:SE:LP:AF:ID  -2.22324e-05:0.000205702:0.0409586:0.839274:rs3131972
1   753405  rs3115860   C   A   .   PASS    AF=0.870226 ES:SE:LP:AF:ID  3.30105e-05:0.000220682:0.0555173:0.870226:rs3115860
1   753541  rs2073813   G   A   .   PASS    AF=0.129404 ES:SE:LP:AF:ID  2.4738e-05:0.000221132:0.0409586:0.129404:rs2073813
1   754063  rs12184312  G   T   .   PASS    AF=0.03701  ES:SE:LP:AF:ID  0.000222504:0.000400787:0.236572:0.03701:rs12184312
1   754105  rs12184325  C   T   .   PASS    AF=0.037247 ES:SE:LP:AF:ID  0.000230126:0.000398277:0.251812:0.037247:rs12184325
1   754182  rs3131969   A   G   .   PASS    AF=0.869577 ES:SE:LP:AF:ID  -2.63948e-05:0.000220254:0.0457575:0.869577:rs3131969
1   754192  rs3131968   A   G   .   PASS    AF=0.869669 ES:SE:LP:AF:ID  -2.78469e-05:0.000220335:0.0457575:0.869669:rs3131968
1   754211  rs12184313  G   A   .   PASS    AF=0.037219 ES:SE:LP:AF:ID  0.000213199:0.00039999:0.229148:0.037219:rs12184313
1   754334  rs3131967   T   C   .   PASS    AF=0.869583 ES:SE:LP:AF:ID  -2.67443e-05:0.000220254:0.0457575:0.869583:rs3131967
1   754433  rs150578204 G   A   .   PASS    AF=0.00512  ES:SE:LP:AF:ID  -0.000572664:0.00112838:0.21467:0.00512:rs150578204
1   754458  rs142682604 G   T   .   PASS    AF=0.005083 ES:SE:LP:AF:ID  -0.000569209:0.00113157:0.21467:0.005083:rs142682604
1   754503  rs3115859   G   A   .   PASS    AF=0.838715 ES:SE:LP:AF:ID  -2.90448e-05:0.000205117:0.05061:0.838715:rs3115859
1   754629  rs10454459  A   G   .   PASS    AF=0.037234 ES:SE:LP:AF:ID  0.000209883:0.000400558:0.221849:0.037234:rs10454459
1   754964  rs3131966   C   T   .   PASS    AF=0.839343 ES:SE:LP:AF:ID  -3.30916e-05:0.000205697:0.0604807:0.839343:rs3131966
1   755240  rs181660517 T   G   .   PASS    AF=0.014031 ES:SE:LP:AF:ID  0.000598355:0.000709409:0.39794:0.014031:rs181660517
1   755435  rs184270342 T   G   .   PASS    AF=0.005588 ES:SE:LP:AF:ID  0.00256362:0.00110322:1.69897:0.005588:rs184270342