Summary

Summary {data-width=650}

Manhattan plot

manhattan_plot

manhattan_plot

QQ plot

qq_plot

qq_plot

AF plot

af_plot

af_plot

P-Z plot

pz_plot

pz_plot

beta_std plot

beta_std_plot

beta_std_plot

Metadata

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}
 

LDSC

*********************************************************************
* LD Score Regression (LDSC)
* Version 1.0.1
* (C) 2014-2019 Brendan Bulik-Sullivan and Hilary Finucane
* Broad Institute of MIT and Harvard / MIT Department of Mathematics
* GNU General Public License v3
*********************************************************************
Call: 
./ldsc.py \
--h2 /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-17907/UKB-b-17907_data.vcf.gz \
--ref-ld-chr ../reference/eur_w_ld_chr/ \
--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-17907/ldsc.txt \
--w-ld-chr ../reference/eur_w_ld_chr/ 

Beginning analysis at Thu Oct 17 14:40:18 2019
Reading summary statistics from /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-17907/UKB-b-17907_data.vcf.gz ...
Read summary statistics for 2704334 SNPs.
Dropped 328 SNPs with duplicated rs numbers.
Reading reference panel LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read reference panel LD Scores for 1290028 SNPs.
Removing partitioned LD Scores with zero variance.
Reading regression weight LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read regression weight LD Scores for 1290028 SNPs.
After merging with reference panel LD, 679818 SNPs remain.
After merging with regression SNP LD, 679818 SNPs remain.
Using two-step estimator with cutoff at 30.
Total Observed scale h2: 0.0008 (0.0013)
Lambda GC: 1.0112
Mean Chi^2: 1.0097
Intercept: 1.0012 (0.0115)
Ratio: 0.1254 (1.1833)
Analysis finished at Thu Oct 17 14:40:55 2019
Total time elapsed: 37.51s

QC metrics

Metrics

Metrics

{
    "af_correlation": 0.7908,
    "inflation_factor": 1,
    "mean_EFFECT": -7.8859e-07,
    "n": "-Inf",
    "n_snps": 9851866,
    "n_clumped_hits": 0,
    "n_p_sig": 0,
    "n_mono": 0,
    "n_ns": 0,
    "n_mac": 0,
    "is_snpid_unique": true,
    "n_miss_EFFECT": 0,
    "n_miss_SE": 0,
    "n_miss_PVAL": 0,
    "n_miss_AF": 0,
    "n_miss_AF_reference": 21441,
    "n_est": "NA",
    "ratio_se_n": "NA",
    "mean_diff": "NaN",
    "ratio_diff": "NaN",
    "sd_y_est1": "NaN",
    "sd_y_est2": "NA",
    "r2_sum1": 0,
    "r2_sum2": 0,
    "r2_sum3": 0,
    "r2_sum4": 0,
    "ldsc_nsnp_merge_refpanel_ld": 679818,
    "ldsc_nsnp_merge_regression_ld": 679818,
    "ldsc_observed_scale_h2_beta": 0.0008,
    "ldsc_observed_scale_h2_se": 0.0013,
    "ldsc_intercept_beta": 1.0012,
    "ldsc_intercept_se": 0.0115,
    "ldsc_lambda_gc": 1.0112,
    "ldsc_mean_chisq": 1.0097,
    "ldsc_ratio": 0.1237
}
 

Flags

name value
af_correlation FALSE
inflation_factor FALSE
n TRUE
is_snpid_non_unique FALSE
mean_EFFECT_nonfinite FALSE
mean_EFFECT_05 FALSE
mean_EFFECT_01 FALSE
mean_chisq FALSE
n_p_sig FALSE
miss_EFFECT FALSE
miss_SE FALSE
miss_PVAL FALSE
ldsc_ratio FALSE
ldsc_intercept_beta FALSE
n_clumped_hits FALSE
r2_sum1 FALSE
r2_sum2 FALSE
r2_sum3 FALSE
r2_sum4 FALSE

Definitions

General metrics

  • af_correlation: Correlation coefficient between AF and AF_reference.
  • inflation_factor (lambda): Genomic inflation factor.
  • mean_EFFECT: Mean of EFFECT size.
  • n: Maximum value of reported sample size across all SNPs, \(n\).
  • n_clumped_hits: Number of clumped hits.
  • n_snps: Number of SNPs
  • n_p_sig: Number of SNPs with pvalue below 5e-8.
  • n_mono: Number of monomorphic (MAF == 1 or MAF == 0) SNPs.
  • n_ns: Number of SNPs with nonsense values:
    • alleles other than A, C, G or T.
    • P-values < 0 or > 1.
    • negative or infinite standard errors (<= 0 or = Infinity).
    • infinite beta estimates or allele frequencies < 0 or > 1.
  • n_mac: Number of cases where MAC (\(2 \times N \times MAF\)) is less than 6.
  • is_snpid_unique: true if the combination of ID REF ALT is unique and therefore no duplication in snpid.
  • n_miss_<*>: Number of NA observations for <*> column.

se_n metrics

  • n_est: Estimated sample size value, \(\widehat{n}\).
  • ratio_se_n: \(\texttt{ratio_se_n} = \frac{\sqrt{\widehat{n}}}{\sqrt{n}}\). We expect ratio_se_n to be 1. When it is not 1, it implies that the trait did not have a variance of 1, the reported sample size is wrong, or that the SNP-level effective sample sizes differ markedly from the reported sample size.
  • mean_diff: \(\texttt{mean_diff} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta_j}{\texttt{n_snps}}\), mean difference between the standardised beta, predicted from P-values, and the observed beta. The difference should be very close to zero if trait has a variance of 1.
    • \(\widehat{\beta_j^{std}} = \sqrt{\frac{{z}_j^2 / ({z}_j^2 + n -2)}{2 \times {MAF}_j \times (1 - {MAF}_j)}} \times sign({z}_j)\),
    • \({z}_j = \frac{\beta_j}{{se}_j}\),
    • and \(\beta_j\) is the reported effect size.
  • ratio_diff: \(\texttt{ratio_diff} = |\frac{\texttt{mean_diff}}{\texttt{mean_diff2}}|\), absolute ratio between the mean of diff and the mean of diff2 (expected difference between the standardised beta predicted from P-values, and the standardised beta derived from the observed beta divided by the predicted SD; NOT reported). The ratio should be close to 1. If different from 1, then implies that the betas are not in a standard deviation scale.
    • \(\texttt{mean_diff2} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta^{\prime}_j}{\texttt{n_snps}}\)
    • \(\beta^{\prime}_j = \frac{\beta_j}{\widehat{\texttt{sd2}}_{y}}\)
  • sd_y_est1: The standard deviation for the trait inferred from the reported sample size, median standard errors for the SNP-trait assocations and SNP variances.
    • \(\widehat{\texttt{sd1}}_{y} = \frac{\sqrt{n} \times median({se}_j)}{C}\),
    • \(C = median(\frac{1}{\sqrt{2 \times {MAF}_j \times (1 - {MAF}_j)}})\),
    • and \({se}_j\) is the reported standard error.
  • sd_y_est2: The standard deviation for the trait inferred from the reported sample size, Z statistics for the SNP-trait effects (beta/se) and allele frequency.
    • \(\widehat{\texttt{sd2}}_{y} = median(\widehat{sd_j})\),
    • \(\widehat{sd_j} = \frac{\beta_j}{\widehat{\beta_j^{std}}}\),

r2 metrics

Sum of variance explained, calculated from the clumped top hits sample.

  • r2_sum<*>: r2 statistics under various assumptions
    • 1: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var1}}}\), \(\texttt{var1} = 1\).
    • 2: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var2}}}\), \(\texttt{var2} = {\widehat{\texttt{sd1}}_{y}}^2\),
    • 3: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var3}}}\), \(\texttt{var3} = {\widehat{\texttt{sd2}}_{y}}^2\),
    • 4: \(r^2 = \sum_j{\frac{F_j}{F_j + n - 2}}\), \(F = \frac{\beta_j^2}{{se}_j^2}\).

LDSC metrics

Metrics from LD regression

  • ldsc_nsnp_merge_refpanel_ld: Number of remaining SNPs after merging with reference panel LD.
  • ldsc_nsnp_merge_regression_ld: Number of remaining SNPs after merging with regression SNP LD.
  • ldsc_observed_scale_h2_{beta,se} Coefficient value and SE for total observed scale h2.
  • ldsc_intercept_{beta,se}: Coefficient value and SE for intercept. Intercept is expected to be 1.
  • ldsc_lambda_gc: Lambda GC statistics.
  • ldsc_mean_chisq: Mean \(\chi^2\) statistics.
  • ldsc_ratio: \(\frac{\texttt{ldsc_intercept_beta} - 1}{\texttt{ldsc_mean_chisq} - 1}\), the proportion of the inflation in the mean \(\chi^2\) that the LD Score regression intercepts ascribes to causes other than polygenic heritability. The value of ratio should be close to zero, though in practice values of 0.1-0.2 are not uncommon, probably due to sample/reference LD Score mismatch or model misspecification (e.g., low LD variants have slightly higher \(h^2\) per SNP).

Flags

When a metric needs attention, the flag should return TRUE.

  • af_correlation: abs(af_correlation) < 0.7.
  • inflation_factor: inflation_factor > 1.2.
  • n: n (max reported sample size) < 10000.
  • is_snpid_non_unique: NOT is_snpid_unique.
  • mean_EFFECT_nonfinite: mean(EFFECT) is NA, NaN, or Inf.
  • mean_EFFECT_05: abs(mean(EFFECT)) > 0.5.
  • mean_EFFECT_01: abs(mean(EFFECT)) > 0.1.
  • mean_chisq: ldsc_mean_chisq > 1.3 or ldsc_mean_chisq < 0.7.
  • n_p_sig: n_p_sig > 1000.
  • miss_<*>: n_miss_<*> / n_snps > 0.01.
  • ldsc_ratio: ldsc_ratio > 0.5
  • ldsc_intercept_beta: ldsc_intercept_beta > 1.5
  • n_clumped_hits: n_clumped_hits > 1000
  • r2_sum<*>: r2_sum<*> > 0.5

Plots

  • Manhattan plot
    • Red line: \(-log_{10}^{5 \times 10^{-8}}\)
    • Blue line: \(-log_{10}^{5 \times 10^{-5}}\)
  • QQ plot
  • AF plot
  • P-Z plot
  • beta_std plot: Scatter plot between \(\widehat{\beta_j^{std}}\) and \(\beta_j\)

Diagnostics

Details

Summary stats

skim_type skim_variable n_missing complete_rate character.min character.max character.empty character.n_unique character.whitespace logical.mean logical.count numeric.mean numeric.sd numeric.p0 numeric.p25 numeric.p50 numeric.p75 numeric.p100 numeric.hist
character ID 0 1.0000000 4 58 0 2704009 0 NA NA NA NA NA NA NA NA NA NA
character REF 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
character ALT 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
logical N 2704334 0.0000000 NA NA NA NA NA NaN : NA NA NA NA NA NA NA NA
numeric CHROM 0 1.0000000 NA NA NA NA NA NA NA 8.662992e+00 5.768808e+00 1.0000e+00 4.000000e+00 8.000000e+00 1.300000e+01 2.200000e+01 ▇▅▃▂▂
numeric POS 0 1.0000000 NA NA NA NA NA NA NA 7.854755e+07 5.663226e+07 5.6870e+03 3.171068e+07 6.895098e+07 1.147307e+08 2.492013e+08 ▇▆▅▂▁
numeric EFFECT 0 1.0000000 NA NA NA NA NA NA NA -8.000000e-07 1.330000e-04 -6.6920e-04 -9.020000e-05 -4.000000e-07 8.870000e-05 7.236000e-04 ▁▂▇▁▁
numeric SE 0 1.0000000 NA NA NA NA NA NA NA 1.319000e-04 8.300000e-06 1.1850e-04 1.248000e-04 1.295000e-04 1.378000e-04 2.704000e-04 ▇▁▁▁▁
numeric PVAL 0 1.0000000 NA NA NA NA NA NA NA 4.980419e-01 2.892090e-01 9.0000e-07 2.500000e-01 5.000000e-01 7.499995e-01 1.000000e+00 ▇▇▇▇▇
numeric PVAL_ztest 0 1.0000000 NA NA NA NA NA NA NA 4.980448e-01 2.891828e-01 9.0000e-07 2.473315e-01 4.970984e-01 7.484986e-01 9.999998e-01 ▇▇▇▇▇
numeric AF 0 1.0000000 NA NA NA NA NA NA NA 4.459498e-01 1.615518e-01 2.1512e-01 3.039220e-01 4.200920e-01 5.743498e-01 7.848800e-01 ▇▆▅▃▃
numeric AF_reference 21441 0.9920716 NA NA NA NA NA NA NA 4.283746e-01 1.827954e-01 1.9970e-04 2.829470e-01 4.099440e-01 5.625000e-01 1.000000e+00 ▂▇▇▃▁

Head and tail

CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
1 49298 rs200943160 T C 0.0000342 0.0002182 0.8800001 0.8755200 0.623763 0.7821490 NA
1 54676 rs2462492 C T -0.0001421 0.0002161 0.5099998 0.5109392 0.400401 NA NA
1 91536 rs6702460 G T -0.0001868 0.0002128 0.3800004 0.3799343 0.456851 0.4207270 NA
1 534192 rs6680723 C T 0.0003133 0.0002431 0.2000000 0.1973754 0.240960 NA NA
1 706368 rs55727773 A G -0.0002884 0.0001509 0.0560003 0.0559976 0.515650 0.2751600 NA
1 763394 rs369924889 G A -0.0001238 0.0001769 0.4799997 0.4842059 0.706753 0.6176120 NA
1 768253 rs2977608 A C -0.0002513 0.0001444 0.0819993 0.0818056 0.761304 0.4894170 NA
1 776546 rs12124819 A G 0.0000059 0.0001613 0.9699999 0.9705771 0.265390 0.0756789 NA
1 808631 rs11240779 G A -0.0000501 0.0001466 0.7300002 0.7322916 0.772626 0.4534740 NA
1 808928 rs11240780 C T -0.0000510 0.0001468 0.7300002 0.7285100 0.772854 0.4522760 NA
CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
22 51180501 rs5770999 T C 0.0000545 0.0001420 0.6999999 0.7010660 0.713658 0.6369810 NA
22 51181919 rs9616825 G C 0.0000617 0.0001413 0.6600001 0.6622151 0.695471 0.6194090 NA
22 51182485 rs6009961 A G 0.0000573 0.0001425 0.6899999 0.6875876 0.715505 0.6383790 NA
22 51186143 rs2879914 T C 0.0000917 0.0001321 0.4899999 0.4875425 0.381826 0.2733630 NA
22 51186228 rs3865766 C T 0.0001352 0.0001288 0.2900000 0.2936967 0.451063 0.4532750 NA
22 51197266 rs61290853 A G 0.0000249 0.0001329 0.8499999 0.8516537 0.386333 0.4229230 NA
22 51198027 rs34939255 A G -0.0000521 0.0001505 0.7300002 0.7293304 0.254557 0.0984425 NA
22 51211106 rs9628250 T C -0.0001677 0.0001492 0.2599998 0.2609190 0.271547 0.1671330 NA
22 51212875 rs2238837 A C 0.0000207 0.0001418 0.8800001 0.8837364 0.331455 0.3724040 NA
22 51237063 rs3896457 T C 0.0000177 0.0001451 0.9000000 0.9031451 0.297971 0.2050720 NA

bcf preview

1   49298   rs10399793  T   C   .   PASS    AF=0.623763 ES:SE:LP:AF:ID  3.41751e-05:0.000218161:0.0555173:0.623763:rs10399793
1   54676   rs2462492   C   T   .   PASS    AF=0.400401 ES:SE:LP:AF:ID  -0.000142078:0.000216129:0.29243:0.400401:rs2462492
1   91536   rs6702460   G   T   .   PASS    AF=0.456851 ES:SE:LP:AF:ID  -0.00018685:0.000212809:0.420216:0.456851:rs6702460
1   534192  rs6680723   C   T   .   PASS    AF=0.24096  ES:SE:LP:AF:ID  0.000313346:0.00024308:0.69897:0.24096:rs6680723
1   706368  rs12029736  A   G   .   PASS    AF=0.51565  ES:SE:LP:AF:ID  -0.00028838:0.000150901:1.25181:0.51565:rs12029736
1   763394  rs3115847   G   A   .   PASS    AF=0.706753 ES:SE:LP:AF:ID  -0.000123763:0.000176917:0.318759:0.706753:rs3115847
1   768253  rs2977608   A   C   .   PASS    AF=0.761304 ES:SE:LP:AF:ID  -0.000251293:0.000144396:1.08619:0.761304:rs2977608
1   776546  rs12124819  A   G   .   PASS    AF=0.26539  ES:SE:LP:AF:ID  5.94921e-06:0.000161293:0.0132283:0.26539:rs12124819
1   808631  rs11240779  G   A   .   PASS    AF=0.772626 ES:SE:LP:AF:ID  -5.01484e-05:0.000146599:0.136677:0.772626:rs11240779
1   808928  rs11240780  C   T   .   PASS    AF=0.772854 ES:SE:LP:AF:ID  -5.09718e-05:0.000146847:0.136677:0.772854:rs11240780
1   814495  rs74461805  C   A   .   PASS    AF=0.340397 ES:SE:LP:AF:ID  -0.000147624:0.000206915:0.318759:0.340397:rs74461805
1   830181  rs28444699  A   G   .   PASS    AF=0.697259 ES:SE:LP:AF:ID  -0.000220844:0.000138432:0.958607:0.697259:rs28444699
1   831489  rs4970385   C   T   .   PASS    AF=0.705403 ES:SE:LP:AF:ID  -0.000270496:0.000135928:1.3279:0.705403:rs4970385
1   831909  rs9697642   C   T   .   PASS    AF=0.705448 ES:SE:LP:AF:ID  -0.000273164:0.000135923:1.35655:0.705448:rs9697642
1   832066  rs9697380   G   C   .   PASS    AF=0.705634 ES:SE:LP:AF:ID  -0.00027541:0.00013593:1.36653:0.705634:rs9697380
1   832318  rs4500250   C   A   .   PASS    AF=0.705662 ES:SE:LP:AF:ID  -0.000273232:0.000135944:1.35655:0.705662:rs4500250
1   832398  rs4553118   T   C   .   PASS    AF=0.730134 ES:SE:LP:AF:ID  -0.000281908:0.000139648:1.35655:0.730134:rs4553118
1   832918  rs28765502  T   C   .   PASS    AF=0.294371 ES:SE:LP:AF:ID  0.000272531:0.000135938:1.34679:0.294371:rs28765502
1   833223  rs13303211  C   T   .   PASS    AF=0.236696 ES:SE:LP:AF:ID  0.000316125:0.000144727:1.5376:0.236696:rs13303211
1   833302  rs28752186  C   T   .   PASS    AF=0.236684 ES:SE:LP:AF:ID  0.000316391:0.000144729:1.5376:0.236684:rs28752186
1   833641  rs28594623  T   C   .   PASS    AF=0.239743 ES:SE:LP:AF:ID  0.000282894:0.000144265:1.30103:0.239743:rs28594623
1   833824  rs28484835  T   C   .   PASS    AF=0.236686 ES:SE:LP:AF:ID  0.000316215:0.000144727:1.5376:0.236686:rs28484835
1   834832  rs4411087   G   C   .   PASS    AF=0.237171 ES:SE:LP:AF:ID  0.000318765:0.000144617:1.55284:0.237171:rs4411087
1   835499  rs4422948   A   G   .   PASS    AF=0.241155 ES:SE:LP:AF:ID  0.00027726:0.000143607:1.26761:0.241155:rs4422948
1   836896  rs28705752  T   C   .   PASS    AF=0.269503 ES:SE:LP:AF:ID  0.000346405:0.00013857:1.92082:0.269503:rs28705752
1   838555  rs4970383   C   A   .   PASS    AF=0.246197 ES:SE:LP:AF:ID  0.000364596:0.00014262:1.95861:0.246197:rs4970383
1   839103  rs28562941  A   G   .   PASS    AF=0.27001  ES:SE:LP:AF:ID  0.000337716:0.000138668:1.82391:0.27001:rs28562941
1   840753  rs4970382   T   C   .   PASS    AF=0.400106 ES:SE:LP:AF:ID  0.000106497:0.000125382:0.39794:0.400106:rs4970382
1   841085  rs1574243   C   G   .   PASS    AF=0.237094 ES:SE:LP:AF:ID  0.000331905:0.000145637:1.63827:0.237094:rs1574243
1   842013  rs7419119   T   G   .   PASS    AF=0.215384 ES:SE:LP:AF:ID  0.000325495:0.000149876:1.52288:0.215384:rs7419119
1   842362  rs28540380  C   T   .   PASS    AF=0.235321 ES:SE:LP:AF:ID  0.000152579:0.000147816:0.522879:0.235321:rs28540380
1   843405  rs11516185  A   G   .   PASS    AF=0.362599 ES:SE:LP:AF:ID  -5.17752e-05:0.000155648:0.130768:0.362599:rs11516185
1   850218  rs6664536   T   A   .   PASS    AF=0.590333 ES:SE:LP:AF:ID  7.05758e-05:0.000125015:0.244125:0.590333:rs6664536
1   850371  rs6679046   G   T   .   PASS    AF=0.603726 ES:SE:LP:AF:ID  1.62103e-05:0.000125717:0.0457575:0.603726:rs6679046
1   850780  rs6657440   C   T   .   PASS    AF=0.603944 ES:SE:LP:AF:ID  1.74033e-05:0.000125699:0.05061:0.603944:rs6657440
1   851204  rs28552953  G   C   .   PASS    AF=0.224621 ES:SE:LP:AF:ID  0.000231209:0.000148716:0.920819:0.224621:rs28552953
1   852037  rs4970463   G   A   .   PASS    AF=0.589688 ES:SE:LP:AF:ID  6.42848e-05:0.000125218:0.21467:0.589688:rs4970463
1   852063  rs28436996  G   A   .   PASS    AF=0.589667 ES:SE:LP:AF:ID  6.46115e-05:0.000125162:0.21467:0.589667:rs28436996
1   852875  rs13303369  C   T   .   PASS    AF=0.607674 ES:SE:LP:AF:ID  -8.46046e-06:0.00012598:0.0222764:0.607674:rs13303369
1   853954  rs1806509   C   A   .   PASS    AF=0.607833 ES:SE:LP:AF:ID  -1.02127e-05:0.000125997:0.0268721:0.607833:rs1806509
1   854777  rs13303019  A   G   .   PASS    AF=0.610318 ES:SE:LP:AF:ID  3.01734e-06:0.000126121:0.00877392:0.610318:rs13303019
1   854978  rs13303057  A   C   .   PASS    AF=0.603286 ES:SE:LP:AF:ID  9.88154e-06:0.000125747:0.0268721:0.603286:rs13303057
1   855075  rs6673914   C   G   .   PASS    AF=0.610338 ES:SE:LP:AF:ID  3.13158e-06:0.000126123:0.00877392:0.610338:rs6673914
1   856099  rs28534711  T   G   .   PASS    AF=0.389935 ES:SE:LP:AF:ID  -2.89166e-06:0.000126147:0.00877392:0.389935:rs28534711
1   856108  rs28742275  A   G   .   PASS    AF=0.389918 ES:SE:LP:AF:ID  -3.16299e-06:0.000126154:0.00877392:0.389918:rs28742275
1   856476  rs4040605   A   G   .   PASS    AF=0.350351 ES:SE:LP:AF:ID  9.89552e-05:0.000129596:0.346787:0.350351:rs4040605
1   858040  rs4970460   C   A   .   PASS    AF=0.217969 ES:SE:LP:AF:ID  0.000179618:0.000149127:0.638272:0.217969:rs4970460
1   858051  rs4970459   C   T   .   PASS    AF=0.219024 ES:SE:LP:AF:ID  0.000176264:0.000148606:0.619789:0.219024:rs4970459
1   858801  rs7418179   A   G   .   PASS    AF=0.765845 ES:SE:LP:AF:ID  -9.12875e-05:0.000145477:0.275724:0.765845:rs7418179
1   860416  rs61464428  G   A   .   PASS    AF=0.766637 ES:SE:LP:AF:ID  -8.58633e-05:0.000145549:0.251812:0.766637:rs61464428