Summary

Summary {data-width=650}

Manhattan plot

manhattan_plot

manhattan_plot

QQ plot

qq_plot

qq_plot

AF plot

af_plot

af_plot

P-Z plot

pz_plot

pz_plot

beta_std plot

beta_std_plot

beta_std_plot

Metadata

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    "gwas_harmonisation_command": "--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/vcf_09_19b/bgzip_vcf/data.batch_20111_100.vcf.gz --id UKB-b:17360 --data /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/raw-output/data.batch_20111_100.txt.gz --cohort_cases 245626 --cohort_controls 119345 --ref /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/human_g1k_v37.fasta --json /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/ukb_gwas.json; 1.1.1",
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}
 

LDSC

*********************************************************************
* LD Score Regression (LDSC)
* Version 1.0.1
* (C) 2014-2019 Brendan Bulik-Sullivan and Hilary Finucane
* Broad Institute of MIT and Harvard / MIT Department of Mathematics
* GNU General Public License v3
*********************************************************************
Call: 
./ldsc.py \
--h2 /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-17360/UKB-b-17360_data.vcf.gz \
--ref-ld-chr ../reference/eur_w_ld_chr/ \
--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-17360/ldsc.txt \
--w-ld-chr ../reference/eur_w_ld_chr/ 

Beginning analysis at Thu Oct 17 14:40:18 2019
Reading summary statistics from /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-17360/UKB-b-17360_data.vcf.gz ...
Read summary statistics for 9629453 SNPs.
Dropped 12714 SNPs with duplicated rs numbers.
Reading reference panel LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read reference panel LD Scores for 1290028 SNPs.
Removing partitioned LD Scores with zero variance.
Reading regression weight LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read regression weight LD Scores for 1290028 SNPs.
After merging with reference panel LD, 1288647 SNPs remain.
After merging with regression SNP LD, 1288647 SNPs remain.
Using two-step estimator with cutoff at 30.
Total Observed scale h2: 0.0334 (0.0022)
Lambda GC: 1.2537
Mean Chi^2: 1.2971
Intercept: 1.0568 (0.0081)
Ratio: 0.1913 (0.0273)
Analysis finished at Thu Oct 17 14:42:08 2019
Total time elapsed: 1.0m:49.15s

QC metrics

Metrics

Metrics

{
    "af_correlation": 0.9494,
    "inflation_factor": 1.1999,
    "mean_EFFECT": -0,
    "n": "-Inf",
    "n_snps": 9851866,
    "n_clumped_hits": 16,
    "n_p_sig": 431,
    "n_mono": 0,
    "n_ns": 0,
    "n_mac": 0,
    "is_snpid_unique": true,
    "n_miss_EFFECT": 0,
    "n_miss_SE": 0,
    "n_miss_PVAL": 0,
    "n_miss_AF": 0,
    "n_miss_AF_reference": 151309,
    "n_est": "NA",
    "ratio_se_n": "NA",
    "mean_diff": "NaN",
    "ratio_diff": "NaN",
    "sd_y_est1": "NaN",
    "sd_y_est2": "NA",
    "r2_sum1": 0,
    "r2_sum2": 0,
    "r2_sum3": 0,
    "r2_sum4": 0,
    "ldsc_nsnp_merge_refpanel_ld": 1288647,
    "ldsc_nsnp_merge_regression_ld": 1288647,
    "ldsc_observed_scale_h2_beta": 0.0334,
    "ldsc_observed_scale_h2_se": 0.0022,
    "ldsc_intercept_beta": 1.0568,
    "ldsc_intercept_se": 0.0081,
    "ldsc_lambda_gc": 1.2537,
    "ldsc_mean_chisq": 1.2971,
    "ldsc_ratio": 0.1912
}
 

Flags

name value
af_correlation FALSE
inflation_factor FALSE
n TRUE
is_snpid_non_unique FALSE
mean_EFFECT_nonfinite FALSE
mean_EFFECT_05 FALSE
mean_EFFECT_01 FALSE
mean_chisq FALSE
n_p_sig FALSE
miss_EFFECT FALSE
miss_SE FALSE
miss_PVAL FALSE
ldsc_ratio FALSE
ldsc_intercept_beta FALSE
n_clumped_hits FALSE
r2_sum1 FALSE
r2_sum2 FALSE
r2_sum3 FALSE
r2_sum4 FALSE

Definitions

General metrics

  • af_correlation: Correlation coefficient between AF and AF_reference.
  • inflation_factor (lambda): Genomic inflation factor.
  • mean_EFFECT: Mean of EFFECT size.
  • n: Maximum value of reported sample size across all SNPs, \(n\).
  • n_clumped_hits: Number of clumped hits.
  • n_snps: Number of SNPs
  • n_p_sig: Number of SNPs with pvalue below 5e-8.
  • n_mono: Number of monomorphic (MAF == 1 or MAF == 0) SNPs.
  • n_ns: Number of SNPs with nonsense values:
    • alleles other than A, C, G or T.
    • P-values < 0 or > 1.
    • negative or infinite standard errors (<= 0 or = Infinity).
    • infinite beta estimates or allele frequencies < 0 or > 1.
  • n_mac: Number of cases where MAC (\(2 \times N \times MAF\)) is less than 6.
  • is_snpid_unique: true if the combination of ID REF ALT is unique and therefore no duplication in snpid.
  • n_miss_<*>: Number of NA observations for <*> column.

se_n metrics

  • n_est: Estimated sample size value, \(\widehat{n}\).
  • ratio_se_n: \(\texttt{ratio_se_n} = \frac{\sqrt{\widehat{n}}}{\sqrt{n}}\). We expect ratio_se_n to be 1. When it is not 1, it implies that the trait did not have a variance of 1, the reported sample size is wrong, or that the SNP-level effective sample sizes differ markedly from the reported sample size.
  • mean_diff: \(\texttt{mean_diff} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta_j}{\texttt{n_snps}}\), mean difference between the standardised beta, predicted from P-values, and the observed beta. The difference should be very close to zero if trait has a variance of 1.
    • \(\widehat{\beta_j^{std}} = \sqrt{\frac{{z}_j^2 / ({z}_j^2 + n -2)}{2 \times {MAF}_j \times (1 - {MAF}_j)}} \times sign({z}_j)\),
    • \({z}_j = \frac{\beta_j}{{se}_j}\),
    • and \(\beta_j\) is the reported effect size.
  • ratio_diff: \(\texttt{ratio_diff} = |\frac{\texttt{mean_diff}}{\texttt{mean_diff2}}|\), absolute ratio between the mean of diff and the mean of diff2 (expected difference between the standardised beta predicted from P-values, and the standardised beta derived from the observed beta divided by the predicted SD; NOT reported). The ratio should be close to 1. If different from 1, then implies that the betas are not in a standard deviation scale.
    • \(\texttt{mean_diff2} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta^{\prime}_j}{\texttt{n_snps}}\)
    • \(\beta^{\prime}_j = \frac{\beta_j}{\widehat{\texttt{sd2}}_{y}}\)
  • sd_y_est1: The standard deviation for the trait inferred from the reported sample size, median standard errors for the SNP-trait assocations and SNP variances.
    • \(\widehat{\texttt{sd1}}_{y} = \frac{\sqrt{n} \times median({se}_j)}{C}\),
    • \(C = median(\frac{1}{\sqrt{2 \times {MAF}_j \times (1 - {MAF}_j)}})\),
    • and \({se}_j\) is the reported standard error.
  • sd_y_est2: The standard deviation for the trait inferred from the reported sample size, Z statistics for the SNP-trait effects (beta/se) and allele frequency.
    • \(\widehat{\texttt{sd2}}_{y} = median(\widehat{sd_j})\),
    • \(\widehat{sd_j} = \frac{\beta_j}{\widehat{\beta_j^{std}}}\),

r2 metrics

Sum of variance explained, calculated from the clumped top hits sample.

  • r2_sum<*>: r2 statistics under various assumptions
    • 1: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var1}}}\), \(\texttt{var1} = 1\).
    • 2: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var2}}}\), \(\texttt{var2} = {\widehat{\texttt{sd1}}_{y}}^2\),
    • 3: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var3}}}\), \(\texttt{var3} = {\widehat{\texttt{sd2}}_{y}}^2\),
    • 4: \(r^2 = \sum_j{\frac{F_j}{F_j + n - 2}}\), \(F = \frac{\beta_j^2}{{se}_j^2}\).

LDSC metrics

Metrics from LD regression

  • ldsc_nsnp_merge_refpanel_ld: Number of remaining SNPs after merging with reference panel LD.
  • ldsc_nsnp_merge_regression_ld: Number of remaining SNPs after merging with regression SNP LD.
  • ldsc_observed_scale_h2_{beta,se} Coefficient value and SE for total observed scale h2.
  • ldsc_intercept_{beta,se}: Coefficient value and SE for intercept. Intercept is expected to be 1.
  • ldsc_lambda_gc: Lambda GC statistics.
  • ldsc_mean_chisq: Mean \(\chi^2\) statistics.
  • ldsc_ratio: \(\frac{\texttt{ldsc_intercept_beta} - 1}{\texttt{ldsc_mean_chisq} - 1}\), the proportion of the inflation in the mean \(\chi^2\) that the LD Score regression intercepts ascribes to causes other than polygenic heritability. The value of ratio should be close to zero, though in practice values of 0.1-0.2 are not uncommon, probably due to sample/reference LD Score mismatch or model misspecification (e.g., low LD variants have slightly higher \(h^2\) per SNP).

Flags

When a metric needs attention, the flag should return TRUE.

  • af_correlation: abs(af_correlation) < 0.7.
  • inflation_factor: inflation_factor > 1.2.
  • n: n (max reported sample size) < 10000.
  • is_snpid_non_unique: NOT is_snpid_unique.
  • mean_EFFECT_nonfinite: mean(EFFECT) is NA, NaN, or Inf.
  • mean_EFFECT_05: abs(mean(EFFECT)) > 0.5.
  • mean_EFFECT_01: abs(mean(EFFECT)) > 0.1.
  • mean_chisq: ldsc_mean_chisq > 1.3 or ldsc_mean_chisq < 0.7.
  • n_p_sig: n_p_sig > 1000.
  • miss_<*>: n_miss_<*> / n_snps > 0.01.
  • ldsc_ratio: ldsc_ratio > 0.5
  • ldsc_intercept_beta: ldsc_intercept_beta > 1.5
  • n_clumped_hits: n_clumped_hits > 1000
  • r2_sum<*>: r2_sum<*> > 0.5

Plots

  • Manhattan plot
    • Red line: \(-log_{10}^{5 \times 10^{-8}}\)
    • Blue line: \(-log_{10}^{5 \times 10^{-5}}\)
  • QQ plot
  • AF plot
  • P-Z plot
  • beta_std plot: Scatter plot between \(\widehat{\beta_j^{std}}\) and \(\beta_j\)

Diagnostics

Details

Summary stats

skim_type skim_variable n_missing complete_rate character.min character.max character.empty character.n_unique character.whitespace logical.mean logical.count numeric.mean numeric.sd numeric.p0 numeric.p25 numeric.p50 numeric.p75 numeric.p100 numeric.hist
character ID 0 1.0000000 3 58 0 9616801 0 NA NA NA NA NA NA NA NA NA NA
character REF 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
character ALT 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
logical N 9629453 0.0000000 NA NA NA NA NA NaN : NA NA NA NA NA NA NA NA
numeric CHROM 0 1.0000000 NA NA NA NA NA NA NA 8.626986e+00 5.750664e+00 1.0000000 4.000000e+00 8.000000e+00 1.300000e+01 2.200000e+01 ▇▅▃▂▂
numeric POS 0 1.0000000 NA NA NA NA NA NA NA 7.883661e+07 5.629601e+07 828.0000000 3.255396e+07 6.943529e+07 1.145686e+08 2.492385e+08 ▇▆▅▂▁
numeric EFFECT 0 1.0000000 NA NA NA NA NA NA NA -1.310000e-05 4.796700e-03 -0.0972477 -1.663700e-03 -3.200000e-06 1.646400e-03 6.188810e-02 ▁▁▂▇▁
numeric SE 0 1.0000000 NA NA NA NA NA NA NA 3.485200e-03 3.048700e-03 0.0010559 1.283300e-03 2.099400e-03 4.707300e-03 5.494600e-02 ▇▁▁▁▁
numeric PVAL 0 1.0000000 NA NA NA NA NA NA NA 4.723814e-01 2.961779e-01 0.0000000 2.099999e-01 4.600002e-01 7.300002e-01 1.000000e+00 ▇▇▇▆▆
numeric PVAL_ztest 0 1.0000000 NA NA NA NA NA NA NA 4.723824e-01 2.961517e-01 0.0000000 2.092875e-01 4.625337e-01 7.287286e-01 1.000000e+00 ▇▆▆▆▆
numeric AF 0 1.0000000 NA NA NA NA NA NA NA 2.073608e-01 2.570667e-01 0.0014250 1.477000e-02 8.348000e-02 3.238790e-01 9.985750e-01 ▇▂▁▁▁
numeric AF_reference 151309 0.9842869 NA NA NA NA NA NA NA 2.095978e-01 2.487172e-01 0.0000000 1.277960e-02 1.038340e-01 3.260780e-01 1.000000e+00 ▇▂▁▁▁

Head and tail

CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
1 49298 rs200943160 T C 0.0007797 0.0019432 0.6899999 0.6882489 0.623819 0.7821490 NA
1 54676 rs2462492 C T -0.0002551 0.0019232 0.8900000 0.8944780 0.400382 NA NA
1 86028 rs114608975 T C -0.0033727 0.0030781 0.2700001 0.2732098 0.103522 0.0277556 NA
1 91536 rs6702460 G T 0.0020216 0.0018959 0.2900000 0.2862825 0.456787 0.4207270 NA
1 234313 rs8179466 C T 0.0017906 0.0037296 0.6300007 0.6311612 0.074565 NA NA
1 534192 rs6680723 C T 0.0002157 0.0021650 0.9199999 0.9206491 0.240950 NA NA
1 546697 rs12025928 A G -0.0025165 0.0027038 0.3500000 0.3519835 0.913569 NA NA
1 693731 rs12238997 A G -0.0023035 0.0018150 0.2000000 0.2043811 0.116165 0.1417730 NA
1 705882 rs72631875 G A -0.0001447 0.0026667 0.9599999 0.9567367 0.067007 0.0315495 NA
1 706368 rs55727773 A G 0.0027615 0.0013440 0.0400000 0.0399040 0.515649 0.2751600 NA
CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
22 51219704 rs147475742 G A 0.0022229 0.0028180 0.4299995 0.4302103 0.042089 0.0473243 NA
22 51219766 rs182321900 C T 0.0137949 0.0131894 0.2999998 0.2956035 0.001917 NA NA
22 51220146 rs868950473 C T 0.0156011 0.0130995 0.2300001 0.2336657 0.001957 NA NA
22 51221190 rs369304721 G A 0.0009680 0.0028130 0.7300002 0.7307687 0.049845 NA NA
22 51221731 rs115055839 T C 0.0014840 0.0021034 0.4799997 0.4804763 0.073490 0.0625000 NA
22 51222100 rs114553188 G T -0.0003664 0.0024893 0.8800001 0.8829905 0.054148 0.0880591 NA
22 51223637 rs375798137 G A 0.0000715 0.0025012 0.9800000 0.9771950 0.053782 0.0788738 NA
22 51229805 rs9616985 T C 0.0016029 0.0021109 0.4500005 0.4476260 0.073334 0.0730831 NA
22 51232488 rs376461333 A G 0.0016417 0.0050123 0.7400005 0.7432723 0.019856 NA NA
22 51237063 rs3896457 T C -0.0020234 0.0012931 0.1199999 0.1176363 0.298101 0.2050720 NA

bcf preview

1   49298   rs10399793  T   C   .   PASS    AF=0.623819 ES:SE:LP:AF:ID  0.000779659:0.00194316:0.161151:0.623819:rs10399793
1   54676   rs2462492   C   T   .   PASS    AF=0.400382 ES:SE:LP:AF:ID  -0.0002551:0.00192325:0.05061:0.400382:rs2462492
1   86028   rs114608975 T   C   .   PASS    AF=0.103522 ES:SE:LP:AF:ID  -0.00337272:0.00307814:0.568636:0.103522:rs114608975
1   91536   rs6702460   G   T   .   PASS    AF=0.456787 ES:SE:LP:AF:ID  0.0020216:0.00189588:0.537602:0.456787:rs6702460
1   234313  rs8179466   C   T   .   PASS    AF=0.074565 ES:SE:LP:AF:ID  0.00179056:0.00372961:0.200659:0.074565:rs8179466
1   534192  rs6680723   C   T   .   PASS    AF=0.24095  ES:SE:LP:AF:ID  0.000215665:0.00216496:0.0362122:0.24095:rs6680723
1   546697  rs12025928  A   G   .   PASS    AF=0.913569 ES:SE:LP:AF:ID  -0.00251654:0.00270378:0.455932:0.913569:rs12025928
1   693731  rs12238997  A   G   .   PASS    AF=0.116165 ES:SE:LP:AF:ID  -0.00230354:0.001815:0.69897:0.116165:rs12238997
1   705882  rs72631875  G   A   .   PASS    AF=0.067007 ES:SE:LP:AF:ID  -0.000144664:0.00266666:0.0177288:0.067007:rs72631875
1   706368  rs12029736  A   G   .   PASS    AF=0.515649 ES:SE:LP:AF:ID  0.00276151:0.00134397:1.39794:0.515649:rs12029736
1   714596  rs149887893 T   C   .   PASS    AF=0.03301  ES:SE:LP:AF:ID  0.000995227:0.00338833:0.113509:0.03301:rs149887893
1   715265  rs12184267  C   T   .   PASS    AF=0.036657 ES:SE:LP:AF:ID  -3.13931e-05:0.00307588:0.00436481:0.036657:rs12184267
1   715367  rs12184277  A   G   .   PASS    AF=0.036772 ES:SE:LP:AF:ID  0.000372932:0.00306433:0.0457575:0.036772:rs12184277
1   717485  rs12184279  C   A   .   PASS    AF=0.036481 ES:SE:LP:AF:ID  0.000152561:0.00308604:0.0177288:0.036481:rs12184279
1   717587  rs144155419 G   A   .   PASS    AF=0.01646  ES:SE:LP:AF:ID  -0.0070182:0.00474476:0.853872:0.01646:rs144155419
1   720381  rs116801199 G   T   .   PASS    AF=0.037012 ES:SE:LP:AF:ID  -0.000262203:0.00305231:0.0315171:0.037012:rs116801199
1   721290  rs12565286  G   C   .   PASS    AF=0.037113 ES:SE:LP:AF:ID  3.99529e-05:0.00304165:0.00436481:0.037113:rs12565286
1   722670  rs116030099 T   C   .   PASS    AF=0.101179 ES:SE:LP:AF:ID  -0.00370088:0.00221849:1.02228:0.101179:rs116030099
1   723891  rs2977670   G   C   .   PASS    AF=0.959057 ES:SE:LP:AF:ID  0.000352048:0.0029332:0.0457575:0.959057:rs2977670
1   724849  rs12126395  C   A   .   PASS    AF=0.031448 ES:SE:LP:AF:ID  -0.0120363:0.00532157:1.61979:0.031448:rs12126395
1   725060  rs865924913 A   T   .   PASS    AF=0.05329  ES:SE:LP:AF:ID  0.000588262:0.00423591:0.05061:0.05329:rs865924913
1   726794  rs28454925  C   G   .   PASS    AF=0.036636 ES:SE:LP:AF:ID  0.000296711:0.00306091:0.0362122:0.036636:rs28454925
1   729632  rs116720794 C   T   .   PASS    AF=0.036961 ES:SE:LP:AF:ID  -0.000136877:0.00303265:0.0177288:0.036961:rs116720794
1   729679  rs4951859   C   G   .   PASS    AF=0.843285 ES:SE:LP:AF:ID  0.00183033:0.00157265:0.619789:0.843285:rs4951859
1   730087  rs148120343 T   C   .   PASS    AF=0.05569  ES:SE:LP:AF:ID  0.00106661:0.0025502:0.167491:0.05569:rs148120343
1   731718  rs58276399  T   C   .   PASS    AF=0.122144 ES:SE:LP:AF:ID  -0.00212895:0.00172176:0.657577:0.122144:rs58276399
1   732989  rs369030935 C   T   .   PASS    AF=0.025767 ES:SE:LP:AF:ID  0.00376494:0.00422894:0.431798:0.025767:rs369030935
1   734349  rs141242758 T   C   .   PASS    AF=0.121384 ES:SE:LP:AF:ID  -0.00216033:0.00172247:0.677781:0.121384:rs141242758
1   736289  rs79010578  T   A   .   PASS    AF=0.13218  ES:SE:LP:AF:ID  -0.00101189:0.00169774:0.259637:0.13218:rs79010578
1   736689  rs181876450 T   C   .   PASS    AF=0.011142 ES:SE:LP:AF:ID  0.00163484:0.00616318:0.102373:0.011142:rs181876450
1   740284  rs61770167  C   T   .   PASS    AF=0.005694 ES:SE:LP:AF:ID  -0.0055182:0.00796543:0.309804:0.005694:rs61770167
1   742813  rs112573343 C   T   .   PASS    AF=0.002269 ES:SE:LP:AF:ID  -0.0125411:0.0133686:0.455932:0.002269:rs112573343
1   752478  rs146277091 G   A   .   PASS    AF=0.036864 ES:SE:LP:AF:ID  -0.000262875:0.00300258:0.0315171:0.036864:rs146277091
1   752566  rs3094315   G   A   .   PASS    AF=0.838933 ES:SE:LP:AF:ID  0.00180078:0.00152253:0.619789:0.838933:rs3094315
1   752721  rs3131972   A   G   .   PASS    AF=0.838573 ES:SE:LP:AF:ID  0.00184844:0.00152094:0.657577:0.838573:rs3131972
1   753405  rs3115860   C   A   .   PASS    AF=0.869835 ES:SE:LP:AF:ID  0.00212436:0.00163222:0.721246:0.869835:rs3115860
1   753541  rs2073813   G   A   .   PASS    AF=0.129808 ES:SE:LP:AF:ID  -0.00217584:0.0016355:0.744727:0.129808:rs2073813
1   754063  rs12184312  G   T   .   PASS    AF=0.037369 ES:SE:LP:AF:ID  0.000663117:0.00295196:0.0861861:0.037369:rs12184312
1   754105  rs12184325  C   T   .   PASS    AF=0.037601 ES:SE:LP:AF:ID  0.000550817:0.00293387:0.0705811:0.037601:rs12184325
1   754182  rs3131969   A   G   .   PASS    AF=0.869187 ES:SE:LP:AF:ID  0.00214074:0.00162911:0.721246:0.869187:rs3131969
1   754192  rs3131968   A   G   .   PASS    AF=0.869287 ES:SE:LP:AF:ID  0.00217411:0.00162979:0.744727:0.869287:rs3131968
1   754211  rs12184313  G   A   .   PASS    AF=0.03758  ES:SE:LP:AF:ID  0.000583262:0.00294573:0.0757207:0.03758:rs12184313
1   754334  rs3131967   T   C   .   PASS    AF=0.869191 ES:SE:LP:AF:ID  0.0021857:0.0016291:0.744727:0.869191:rs3131967
1   754433  rs150578204 G   A   .   PASS    AF=0.005133 ES:SE:LP:AF:ID  -0.00427402:0.00835161:0.21467:0.005133:rs150578204
1   754458  rs142682604 G   T   .   PASS    AF=0.005099 ES:SE:LP:AF:ID  -0.00436337:0.00837395:0.221849:0.005099:rs142682604
1   754503  rs3115859   G   A   .   PASS    AF=0.838023 ES:SE:LP:AF:ID  0.00182133:0.00151675:0.638272:0.838023:rs3115859
1   754629  rs10454459  A   G   .   PASS    AF=0.037588 ES:SE:LP:AF:ID  0.000570934:0.00295019:0.0705811:0.037588:rs10454459
1   754964  rs3131966   C   T   .   PASS    AF=0.838649 ES:SE:LP:AF:ID  0.00178403:0.00152104:0.619789:0.838649:rs3131966
1   755240  rs181660517 T   G   .   PASS    AF=0.013796 ES:SE:LP:AF:ID  0.00402338:0.005305:0.346787:0.013796:rs181660517
1   755435  rs184270342 T   G   .   PASS    AF=0.005564 ES:SE:LP:AF:ID  -0.00151278:0.00818465:0.0705811:0.005564:rs184270342