Summary

Summary {data-width=650}

Manhattan plot

manhattan_plot

manhattan_plot

QQ plot

qq_plot

qq_plot

AF plot

af_plot

af_plot

P-Z plot

pz_plot

pz_plot

beta_std plot

beta_std_plot

beta_std_plot

Metadata

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    "gwas_harmonisation_command": "--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/vcf_09_19b/bgzip_vcf/data.batch_3669.vcf.gz --id UKB-b:1226 --data /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/raw-output/data.batch_3669.txt.gz --cohort_controls 12249 --ref /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/human_g1k_v37.fasta --json /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/ukb_gwas.json; 1.1.1",
    "file_date": "2019-09-13T03:44:17.425012",
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}
 

LDSC

*********************************************************************
* LD Score Regression (LDSC)
* Version 1.0.1
* (C) 2014-2019 Brendan Bulik-Sullivan and Hilary Finucane
* Broad Institute of MIT and Harvard / MIT Department of Mathematics
* GNU General Public License v3
*********************************************************************
Call: 
./ldsc.py \
--h2 /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-1226/UKB-b-1226_data.vcf.gz \
--ref-ld-chr ../reference/eur_w_ld_chr/ \
--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-1226/ldsc.txt \
--w-ld-chr ../reference/eur_w_ld_chr/ 

Beginning analysis at Thu Oct 17 14:41:34 2019
Reading summary statistics from /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-1226/UKB-b-1226_data.vcf.gz ...
Read summary statistics for 6247006 SNPs.
Dropped 3107 SNPs with duplicated rs numbers.
Reading reference panel LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read reference panel LD Scores for 1290028 SNPs.
Removing partitioned LD Scores with zero variance.
Reading regression weight LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read regression weight LD Scores for 1290028 SNPs.
After merging with reference panel LD, 1230677 SNPs remain.
After merging with regression SNP LD, 1230677 SNPs remain.
Using two-step estimator with cutoff at 30.
Total Observed scale h2: 0.0402 (0.0417)
Lambda GC: 1.0113
Mean Chi^2: 1.0148
Intercept: 1.0046 (0.0071)
Ratio: 0.309 (0.4797)
Analysis finished at Thu Oct 17 14:42:50 2019
Total time elapsed: 1.0m:16.38s

QC metrics

Metrics

Metrics

{
    "af_correlation": 0.9285,
    "inflation_factor": 1,
    "mean_EFFECT": 0,
    "n": "-Inf",
    "n_snps": 9851866,
    "n_clumped_hits": 0,
    "n_p_sig": 0,
    "n_mono": 0,
    "n_ns": 0,
    "n_mac": 0,
    "is_snpid_unique": true,
    "n_miss_EFFECT": 0,
    "n_miss_SE": 0,
    "n_miss_PVAL": 0,
    "n_miss_AF": 0,
    "n_miss_AF_reference": 57104,
    "n_est": "NA",
    "ratio_se_n": "NA",
    "mean_diff": "NaN",
    "ratio_diff": "NaN",
    "sd_y_est1": "NaN",
    "sd_y_est2": "NA",
    "r2_sum1": 0,
    "r2_sum2": 0,
    "r2_sum3": 0,
    "r2_sum4": 0,
    "ldsc_nsnp_merge_refpanel_ld": 1230677,
    "ldsc_nsnp_merge_regression_ld": 1230677,
    "ldsc_observed_scale_h2_beta": 0.0402,
    "ldsc_observed_scale_h2_se": 0.0417,
    "ldsc_intercept_beta": 1.0046,
    "ldsc_intercept_se": 0.0071,
    "ldsc_lambda_gc": 1.0113,
    "ldsc_mean_chisq": 1.0148,
    "ldsc_ratio": 0.3108
}
 

Flags

name value
af_correlation FALSE
inflation_factor FALSE
n TRUE
is_snpid_non_unique FALSE
mean_EFFECT_nonfinite FALSE
mean_EFFECT_05 FALSE
mean_EFFECT_01 FALSE
mean_chisq FALSE
n_p_sig FALSE
miss_EFFECT FALSE
miss_SE FALSE
miss_PVAL FALSE
ldsc_ratio FALSE
ldsc_intercept_beta FALSE
n_clumped_hits FALSE
r2_sum1 FALSE
r2_sum2 FALSE
r2_sum3 FALSE
r2_sum4 FALSE

Definitions

General metrics

  • af_correlation: Correlation coefficient between AF and AF_reference.
  • inflation_factor (lambda): Genomic inflation factor.
  • mean_EFFECT: Mean of EFFECT size.
  • n: Maximum value of reported sample size across all SNPs, \(n\).
  • n_clumped_hits: Number of clumped hits.
  • n_snps: Number of SNPs
  • n_p_sig: Number of SNPs with pvalue below 5e-8.
  • n_mono: Number of monomorphic (MAF == 1 or MAF == 0) SNPs.
  • n_ns: Number of SNPs with nonsense values:
    • alleles other than A, C, G or T.
    • P-values < 0 or > 1.
    • negative or infinite standard errors (<= 0 or = Infinity).
    • infinite beta estimates or allele frequencies < 0 or > 1.
  • n_mac: Number of cases where MAC (\(2 \times N \times MAF\)) is less than 6.
  • is_snpid_unique: true if the combination of ID REF ALT is unique and therefore no duplication in snpid.
  • n_miss_<*>: Number of NA observations for <*> column.

se_n metrics

  • n_est: Estimated sample size value, \(\widehat{n}\).
  • ratio_se_n: \(\texttt{ratio_se_n} = \frac{\sqrt{\widehat{n}}}{\sqrt{n}}\). We expect ratio_se_n to be 1. When it is not 1, it implies that the trait did not have a variance of 1, the reported sample size is wrong, or that the SNP-level effective sample sizes differ markedly from the reported sample size.
  • mean_diff: \(\texttt{mean_diff} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta_j}{\texttt{n_snps}}\), mean difference between the standardised beta, predicted from P-values, and the observed beta. The difference should be very close to zero if trait has a variance of 1.
    • \(\widehat{\beta_j^{std}} = \sqrt{\frac{{z}_j^2 / ({z}_j^2 + n -2)}{2 \times {MAF}_j \times (1 - {MAF}_j)}} \times sign({z}_j)\),
    • \({z}_j = \frac{\beta_j}{{se}_j}\),
    • and \(\beta_j\) is the reported effect size.
  • ratio_diff: \(\texttt{ratio_diff} = |\frac{\texttt{mean_diff}}{\texttt{mean_diff2}}|\), absolute ratio between the mean of diff and the mean of diff2 (expected difference between the standardised beta predicted from P-values, and the standardised beta derived from the observed beta divided by the predicted SD; NOT reported). The ratio should be close to 1. If different from 1, then implies that the betas are not in a standard deviation scale.
    • \(\texttt{mean_diff2} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta^{\prime}_j}{\texttt{n_snps}}\)
    • \(\beta^{\prime}_j = \frac{\beta_j}{\widehat{\texttt{sd2}}_{y}}\)
  • sd_y_est1: The standard deviation for the trait inferred from the reported sample size, median standard errors for the SNP-trait assocations and SNP variances.
    • \(\widehat{\texttt{sd1}}_{y} = \frac{\sqrt{n} \times median({se}_j)}{C}\),
    • \(C = median(\frac{1}{\sqrt{2 \times {MAF}_j \times (1 - {MAF}_j)}})\),
    • and \({se}_j\) is the reported standard error.
  • sd_y_est2: The standard deviation for the trait inferred from the reported sample size, Z statistics for the SNP-trait effects (beta/se) and allele frequency.
    • \(\widehat{\texttt{sd2}}_{y} = median(\widehat{sd_j})\),
    • \(\widehat{sd_j} = \frac{\beta_j}{\widehat{\beta_j^{std}}}\),

r2 metrics

Sum of variance explained, calculated from the clumped top hits sample.

  • r2_sum<*>: r2 statistics under various assumptions
    • 1: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var1}}}\), \(\texttt{var1} = 1\).
    • 2: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var2}}}\), \(\texttt{var2} = {\widehat{\texttt{sd1}}_{y}}^2\),
    • 3: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var3}}}\), \(\texttt{var3} = {\widehat{\texttt{sd2}}_{y}}^2\),
    • 4: \(r^2 = \sum_j{\frac{F_j}{F_j + n - 2}}\), \(F = \frac{\beta_j^2}{{se}_j^2}\).

LDSC metrics

Metrics from LD regression

  • ldsc_nsnp_merge_refpanel_ld: Number of remaining SNPs after merging with reference panel LD.
  • ldsc_nsnp_merge_regression_ld: Number of remaining SNPs after merging with regression SNP LD.
  • ldsc_observed_scale_h2_{beta,se} Coefficient value and SE for total observed scale h2.
  • ldsc_intercept_{beta,se}: Coefficient value and SE for intercept. Intercept is expected to be 1.
  • ldsc_lambda_gc: Lambda GC statistics.
  • ldsc_mean_chisq: Mean \(\chi^2\) statistics.
  • ldsc_ratio: \(\frac{\texttt{ldsc_intercept_beta} - 1}{\texttt{ldsc_mean_chisq} - 1}\), the proportion of the inflation in the mean \(\chi^2\) that the LD Score regression intercepts ascribes to causes other than polygenic heritability. The value of ratio should be close to zero, though in practice values of 0.1-0.2 are not uncommon, probably due to sample/reference LD Score mismatch or model misspecification (e.g., low LD variants have slightly higher \(h^2\) per SNP).

Flags

When a metric needs attention, the flag should return TRUE.

  • af_correlation: abs(af_correlation) < 0.7.
  • inflation_factor: inflation_factor > 1.2.
  • n: n (max reported sample size) < 10000.
  • is_snpid_non_unique: NOT is_snpid_unique.
  • mean_EFFECT_nonfinite: mean(EFFECT) is NA, NaN, or Inf.
  • mean_EFFECT_05: abs(mean(EFFECT)) > 0.5.
  • mean_EFFECT_01: abs(mean(EFFECT)) > 0.1.
  • mean_chisq: ldsc_mean_chisq > 1.3 or ldsc_mean_chisq < 0.7.
  • n_p_sig: n_p_sig > 1000.
  • miss_<*>: n_miss_<*> / n_snps > 0.01.
  • ldsc_ratio: ldsc_ratio > 0.5
  • ldsc_intercept_beta: ldsc_intercept_beta > 1.5
  • n_clumped_hits: n_clumped_hits > 1000
  • r2_sum<*>: r2_sum<*> > 0.5

Plots

  • Manhattan plot
    • Red line: \(-log_{10}^{5 \times 10^{-8}}\)
    • Blue line: \(-log_{10}^{5 \times 10^{-5}}\)
  • QQ plot
  • AF plot
  • P-Z plot
  • beta_std plot: Scatter plot between \(\widehat{\beta_j^{std}}\) and \(\beta_j\)

Diagnostics

Details

Summary stats

skim_type skim_variable n_missing complete_rate character.min character.max character.empty character.n_unique character.whitespace logical.mean logical.count numeric.mean numeric.sd numeric.p0 numeric.p25 numeric.p50 numeric.p75 numeric.p100 numeric.hist
character ID 0 1.000000 3 58 0 6243919 0 NA NA NA NA NA NA NA NA NA NA
character REF 0 1.000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
character ALT 0 1.000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
logical N 6247006 0.000000 NA NA NA NA NA NaN : NA NA NA NA NA NA NA NA
numeric CHROM 0 1.000000 NA NA NA NA NA NA NA 8.667740e+00 5.763082e+00 1.0000000 4.000000e+00 8.000000e+00 1.300000e+01 2.200000e+01 ▇▅▅▂▂
numeric POS 0 1.000000 NA NA NA NA NA NA NA 7.859416e+07 5.652003e+07 828.0000000 3.200410e+07 6.901493e+07 1.145279e+08 2.492385e+08 ▇▆▅▂▁
numeric EFFECT 0 1.000000 NA NA NA NA NA NA NA 3.760000e-05 1.652950e-02 -0.1721080 -9.570600e-03 8.100000e-06 9.631800e-03 1.656320e-01 ▁▁▇▁▁
numeric SE 0 1.000000 NA NA NA NA NA NA NA 1.540770e-02 5.638300e-03 0.0096537 1.079700e-02 1.317030e-02 1.865440e-02 6.042220e-02 ▇▂▁▁▁
numeric PVAL 0 1.000000 NA NA NA NA NA NA NA 4.970045e-01 2.899264e-01 0.0000008 2.500000e-01 5.000000e-01 7.499995e-01 1.000000e+00 ▇▇▇▇▇
numeric PVAL_ztest 0 1.000000 NA NA NA NA NA NA NA 4.970059e-01 2.899005e-01 0.0000008 2.450554e-01 4.957434e-01 7.484053e-01 9.999999e-01 ▇▇▇▇▇
numeric AF 0 1.000000 NA NA NA NA NA NA NA 3.024141e-01 2.554552e-01 0.0285740 8.651200e-02 2.169160e-01 4.660610e-01 9.714260e-01 ▇▃▂▂▁
numeric AF_reference 57104 0.990859 NA NA NA NA NA NA NA 2.995302e-01 2.482434e-01 0.0000000 9.584660e-02 2.252400e-01 4.572680e-01 1.000000e+00 ▇▅▂▂▁

Head and tail

CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
1 49298 rs200943160 T C 0.0298874 0.0180160 0.0969996 0.0971286 0.623162 0.7821490 NA
1 54676 rs2462492 C T 0.0056290 0.0177262 0.7499995 0.7508235 0.400192 NA NA
1 86028 rs114608975 T C -0.0029599 0.0279619 0.9199999 0.9156979 0.104031 0.0277556 NA
1 91536 rs6702460 G T 0.0224627 0.0173902 0.2000000 0.1964655 0.456779 0.4207270 NA
1 234313 rs8179466 C T 0.0421129 0.0340561 0.2200002 0.2162451 0.074129 NA NA
1 534192 rs6680723 C T -0.0091426 0.0198067 0.6400000 0.6443737 0.243219 NA NA
1 546697 rs12025928 A G 0.0183831 0.0251595 0.4600002 0.4649854 0.915474 NA NA
1 693731 rs12238997 A G 0.0265333 0.0166940 0.1100001 0.1119721 0.115862 0.1417730 NA
1 705882 rs72631875 G A -0.0263762 0.0244118 0.2800000 0.2799333 0.067348 0.0315495 NA
1 706368 rs55727773 A G -0.0361663 0.0124032 0.0035000 0.0035468 0.517383 0.2751600 NA
CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
22 51218377 rs2519461 G C -0.0146312 0.0193160 0.4500005 0.4487711 0.074471 0.0826677 NA
22 51219006 rs28729663 G A -0.0145522 0.0147802 0.3200000 0.3248334 0.141115 0.2052720 NA
22 51219387 rs9616832 T C -0.0119785 0.0193770 0.5400003 0.5364558 0.074480 0.0654952 NA
22 51219704 rs147475742 G A -0.0264327 0.0262865 0.3100002 0.3146264 0.041016 0.0473243 NA
22 51221190 rs369304721 G A -0.0270018 0.0260851 0.2999998 0.3006023 0.049340 NA NA
22 51221731 rs115055839 T C -0.0128656 0.0193909 0.5099998 0.5070190 0.073821 0.0625000 NA
22 51222100 rs114553188 G T -0.0095619 0.0222637 0.6700003 0.6675705 0.056586 0.0880591 NA
22 51223637 rs375798137 G A -0.0115677 0.0223412 0.5999997 0.6046157 0.056317 0.0788738 NA
22 51229805 rs9616985 T C -0.0153519 0.0194610 0.4299995 0.4301970 0.073629 0.0730831 NA
22 51237063 rs3896457 T C 0.0054389 0.0119465 0.6499995 0.6489158 0.295378 0.2050720 NA

bcf preview

1   49298   rs10399793  T   C   .   PASS    AF=0.623162 ES:SE:LP:AF:ID  0.0298874:0.018016:1.01323:0.623162:rs10399793
1   54676   rs2462492   C   T   .   PASS    AF=0.400192 ES:SE:LP:AF:ID  0.00562902:0.0177262:0.124939:0.400192:rs2462492
1   86028   rs114608975 T   C   .   PASS    AF=0.104031 ES:SE:LP:AF:ID  -0.00295989:0.0279619:0.0362122:0.104031:rs114608975
1   91536   rs6702460   G   T   .   PASS    AF=0.456779 ES:SE:LP:AF:ID  0.0224627:0.0173902:0.69897:0.456779:rs6702460
1   234313  rs8179466   C   T   .   PASS    AF=0.074129 ES:SE:LP:AF:ID  0.0421129:0.0340561:0.657577:0.074129:rs8179466
1   534192  rs6680723   C   T   .   PASS    AF=0.243219 ES:SE:LP:AF:ID  -0.00914262:0.0198067:0.19382:0.243219:rs6680723
1   546697  rs12025928  A   G   .   PASS    AF=0.915474 ES:SE:LP:AF:ID  0.0183831:0.0251595:0.337242:0.915474:rs12025928
1   693731  rs12238997  A   G   .   PASS    AF=0.115862 ES:SE:LP:AF:ID  0.0265333:0.016694:0.958607:0.115862:rs12238997
1   705882  rs72631875  G   A   .   PASS    AF=0.067348 ES:SE:LP:AF:ID  -0.0263762:0.0244118:0.552842:0.067348:rs72631875
1   706368  rs12029736  A   G   .   PASS    AF=0.517383 ES:SE:LP:AF:ID  -0.0361663:0.0124032:2.45593:0.517383:rs12029736
1   714596  rs149887893 T   C   .   PASS    AF=0.034213 ES:SE:LP:AF:ID  0.038234:0.0306883:0.677781:0.034213:rs149887893
1   715265  rs12184267  C   T   .   PASS    AF=0.037663 ES:SE:LP:AF:ID  0.0330458:0.0279856:0.619789:0.037663:rs12184267
1   715367  rs12184277  A   G   .   PASS    AF=0.037877 ES:SE:LP:AF:ID  0.0328993:0.0278453:0.619789:0.037877:rs12184277
1   717485  rs12184279  C   A   .   PASS    AF=0.037528 ES:SE:LP:AF:ID  0.0375691:0.0280829:0.744727:0.037528:rs12184279
1   720381  rs116801199 G   T   .   PASS    AF=0.038041 ES:SE:LP:AF:ID  0.0349721:0.0277851:0.677781:0.038041:rs116801199
1   721290  rs12565286  G   C   .   PASS    AF=0.038282 ES:SE:LP:AF:ID  0.035067:0.0276226:0.69897:0.038282:rs12565286
1   722670  rs116030099 T   C   .   PASS    AF=0.098805 ES:SE:LP:AF:ID  0.0247823:0.020591:0.638272:0.098805:rs116030099
1   723891  rs2977670   G   C   .   PASS    AF=0.957348 ES:SE:LP:AF:ID  -0.0268414:0.0265016:0.508638:0.957348:rs2977670
1   724849  rs12126395  C   A   .   PASS    AF=0.030171 ES:SE:LP:AF:ID  -0.0296569:0.0511882:0.251812:0.030171:rs12126395
1   725060  rs865924913 A   T   .   PASS    AF=0.054078 ES:SE:LP:AF:ID  -0.0184195:0.038209:0.200659:0.054078:rs865924913
1   726794  rs28454925  C   G   .   PASS    AF=0.037606 ES:SE:LP:AF:ID  0.0404152:0.0279049:0.823909:0.037606:rs28454925
1   729632  rs116720794 C   T   .   PASS    AF=0.038006 ES:SE:LP:AF:ID  0.0380498:0.0276039:0.769551:0.038006:rs116720794
1   729679  rs4951859   C   G   .   PASS    AF=0.841661 ES:SE:LP:AF:ID  -0.0270713:0.0144237:1.21467:0.841661:rs4951859
1   730087  rs148120343 T   C   .   PASS    AF=0.055993 ES:SE:LP:AF:ID  0.0257024:0.0232782:0.568636:0.055993:rs148120343
1   731718  rs58276399  T   C   .   PASS    AF=0.122272 ES:SE:LP:AF:ID  0.0277742:0.0158177:1.10237:0.122272:rs58276399
1   734349  rs141242758 T   C   .   PASS    AF=0.121404 ES:SE:LP:AF:ID  0.0292151:0.015824:1.18709:0.121404:rs141242758
1   736289  rs79010578  T   A   .   PASS    AF=0.132559 ES:SE:LP:AF:ID  0.0234441:0.0155896:0.886057:0.132559:rs79010578
1   752478  rs146277091 G   A   .   PASS    AF=0.038028 ES:SE:LP:AF:ID  0.03695:0.0272432:0.744727:0.038028:rs146277091
1   752566  rs3094315   G   A   .   PASS    AF=0.838651 ES:SE:LP:AF:ID  -0.0333772:0.0140179:1.76955:0.838651:rs3094315
1   752721  rs3131972   A   G   .   PASS    AF=0.837864 ES:SE:LP:AF:ID  -0.0324798:0.0139933:1.69897:0.837864:rs3131972
1   753405  rs3115860   C   A   .   PASS    AF=0.870665 ES:SE:LP:AF:ID  -0.0338379:0.0150413:1.61979:0.870665:rs3115860
1   753541  rs2073813   G   A   .   PASS    AF=0.129576 ES:SE:LP:AF:ID  0.0334813:0.0150635:1.58503:0.129576:rs2073813
1   754063  rs12184312  G   T   .   PASS    AF=0.038631 ES:SE:LP:AF:ID  0.0346285:0.0267544:0.69897:0.038631:rs12184312
1   754105  rs12184325  C   T   .   PASS    AF=0.038738 ES:SE:LP:AF:ID  0.0332349:0.0266492:0.677781:0.038738:rs12184325
1   754182  rs3131969   A   G   .   PASS    AF=0.869595 ES:SE:LP:AF:ID  -0.0322667:0.0149996:1.50864:0.869595:rs3131969
1   754192  rs3131968   A   G   .   PASS    AF=0.869636 ES:SE:LP:AF:ID  -0.031993:0.0150014:1.48149:0.869636:rs3131968
1   754211  rs12184313  G   A   .   PASS    AF=0.038801 ES:SE:LP:AF:ID  0.0335767:0.0266965:0.677781:0.038801:rs12184313
1   754334  rs3131967   T   C   .   PASS    AF=0.869598 ES:SE:LP:AF:ID  -0.0322656:0.0149991:1.50864:0.869598:rs3131967
1   754503  rs3115859   G   A   .   PASS    AF=0.83728  ES:SE:LP:AF:ID  -0.0317805:0.0139493:1.63827:0.83728:rs3115859
1   754629  rs10454459  A   G   .   PASS    AF=0.038803 ES:SE:LP:AF:ID  0.0332551:0.0267246:0.677781:0.038803:rs10454459
1   754964  rs3131966   C   T   .   PASS    AF=0.838107 ES:SE:LP:AF:ID  -0.0311313:0.0139947:1.58503:0.838107:rs3131966
1   755775  rs3131965   A   G   .   PASS    AF=0.839591 ES:SE:LP:AF:ID  -0.0325694:0.014181:1.65758:0.839591:rs3131965
1   755890  rs3115858   A   T   .   PASS    AF=0.870092 ES:SE:LP:AF:ID  -0.0341424:0.0149829:1.63827:0.870092:rs3115858
1   756604  rs3131962   A   G   .   PASS    AF=0.869704 ES:SE:LP:AF:ID  -0.0342668:0.0149442:1.65758:0.869704:rs3131962
1   757640  rs3115853   G   A   .   PASS    AF=0.86821  ES:SE:LP:AF:ID  -0.0324326:0.0149072:1.52288:0.86821:rs3115853
1   757734  rs4951929   C   T   .   PASS    AF=0.869872 ES:SE:LP:AF:ID  -0.0341609:0.0149615:1.65758:0.869872:rs4951929
1   757936  rs4951862   C   A   .   PASS    AF=0.869895 ES:SE:LP:AF:ID  -0.034258:0.0149627:1.65758:0.869895:rs4951862
1   758144  rs3131956   A   G   .   PASS    AF=0.869896 ES:SE:LP:AF:ID  -0.034242:0.0149625:1.65758:0.869896:rs3131956
1   758626  rs3131954   C   T   .   PASS    AF=0.870416 ES:SE:LP:AF:ID  -0.0331938:0.0150093:1.56864:0.870416:rs3131954
1   759036  rs114525117 G   A   .   PASS    AF=0.038656 ES:SE:LP:AF:ID  0.0309108:0.0266746:0.60206:0.038656:rs114525117