Summary

Summary {data-width=650}

Manhattan plot

manhattan_plot

manhattan_plot

QQ plot

qq_plot

qq_plot

AF plot

af_plot

af_plot

P-Z plot

pz_plot

pz_plot

beta_std plot

beta_std_plot

beta_std_plot

Metadata

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    "gwas_harmonisation_command": "--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/vcf_09_19b/bgzip_vcf/data.batch_20110_6.vcf.gz --id UKB-b:12018 --data /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/raw-output/data.batch_20110_6.txt.gz --cohort_cases 25314 --cohort_controls 398378 --ref /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/human_g1k_v37.fasta --json /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/ukb_gwas.json; 1.1.1",
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}
 

LDSC

*********************************************************************
* LD Score Regression (LDSC)
* Version 1.0.1
* (C) 2014-2019 Brendan Bulik-Sullivan and Hilary Finucane
* Broad Institute of MIT and Harvard / MIT Department of Mathematics
* GNU General Public License v3
*********************************************************************
Call: 
./ldsc.py \
--h2 /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-12018/UKB-b-12018_data.vcf.gz \
--ref-ld-chr ../reference/eur_w_ld_chr/ \
--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-12018/ldsc.txt \
--w-ld-chr ../reference/eur_w_ld_chr/ 

Beginning analysis at Thu Oct 17 14:41:11 2019
Reading summary statistics from /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-12018/UKB-b-12018_data.vcf.gz ...
Read summary statistics for 7268332 SNPs.
Dropped 4743 SNPs with duplicated rs numbers.
Reading reference panel LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read reference panel LD Scores for 1290028 SNPs.
Removing partitioned LD Scores with zero variance.
Reading regression weight LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read regression weight LD Scores for 1290028 SNPs.
After merging with reference panel LD, 1271036 SNPs remain.
After merging with regression SNP LD, 1271036 SNPs remain.
Using two-step estimator with cutoff at 30.
Total Observed scale h2: 0.0138 (0.0016)
Lambda GC: 1.1462
Mean Chi^2: 1.1664
Intercept: 1.051 (0.0075)
Ratio: 0.3065 (0.0449)
Analysis finished at Thu Oct 17 14:42:44 2019
Total time elapsed: 1.0m:32.9s

QC metrics

Metrics

Metrics

{
    "af_correlation": 0.938,
    "inflation_factor": 1.1474,
    "mean_EFFECT": 6.1075e-06,
    "n": "-Inf",
    "n_snps": 9851866,
    "n_clumped_hits": 4,
    "n_p_sig": 118,
    "n_mono": 0,
    "n_ns": 0,
    "n_mac": 0,
    "is_snpid_unique": true,
    "n_miss_EFFECT": 0,
    "n_miss_SE": 0,
    "n_miss_PVAL": 0,
    "n_miss_AF": 0,
    "n_miss_AF_reference": 67125,
    "n_est": "NA",
    "ratio_se_n": "NA",
    "mean_diff": "NaN",
    "ratio_diff": "NaN",
    "sd_y_est1": "NaN",
    "sd_y_est2": "NA",
    "r2_sum1": 0,
    "r2_sum2": 0,
    "r2_sum3": 0,
    "r2_sum4": 0,
    "ldsc_nsnp_merge_refpanel_ld": 1271036,
    "ldsc_nsnp_merge_regression_ld": 1271036,
    "ldsc_observed_scale_h2_beta": 0.0138,
    "ldsc_observed_scale_h2_se": 0.0016,
    "ldsc_intercept_beta": 1.051,
    "ldsc_intercept_se": 0.0075,
    "ldsc_lambda_gc": 1.1462,
    "ldsc_mean_chisq": 1.1664,
    "ldsc_ratio": 0.3065
}
 

Flags

name value
af_correlation FALSE
inflation_factor FALSE
n TRUE
is_snpid_non_unique FALSE
mean_EFFECT_nonfinite FALSE
mean_EFFECT_05 FALSE
mean_EFFECT_01 FALSE
mean_chisq FALSE
n_p_sig FALSE
miss_EFFECT FALSE
miss_SE FALSE
miss_PVAL FALSE
ldsc_ratio FALSE
ldsc_intercept_beta FALSE
n_clumped_hits FALSE
r2_sum1 FALSE
r2_sum2 FALSE
r2_sum3 FALSE
r2_sum4 FALSE

Definitions

General metrics

  • af_correlation: Correlation coefficient between AF and AF_reference.
  • inflation_factor (lambda): Genomic inflation factor.
  • mean_EFFECT: Mean of EFFECT size.
  • n: Maximum value of reported sample size across all SNPs, \(n\).
  • n_clumped_hits: Number of clumped hits.
  • n_snps: Number of SNPs
  • n_p_sig: Number of SNPs with pvalue below 5e-8.
  • n_mono: Number of monomorphic (MAF == 1 or MAF == 0) SNPs.
  • n_ns: Number of SNPs with nonsense values:
    • alleles other than A, C, G or T.
    • P-values < 0 or > 1.
    • negative or infinite standard errors (<= 0 or = Infinity).
    • infinite beta estimates or allele frequencies < 0 or > 1.
  • n_mac: Number of cases where MAC (\(2 \times N \times MAF\)) is less than 6.
  • is_snpid_unique: true if the combination of ID REF ALT is unique and therefore no duplication in snpid.
  • n_miss_<*>: Number of NA observations for <*> column.

se_n metrics

  • n_est: Estimated sample size value, \(\widehat{n}\).
  • ratio_se_n: \(\texttt{ratio_se_n} = \frac{\sqrt{\widehat{n}}}{\sqrt{n}}\). We expect ratio_se_n to be 1. When it is not 1, it implies that the trait did not have a variance of 1, the reported sample size is wrong, or that the SNP-level effective sample sizes differ markedly from the reported sample size.
  • mean_diff: \(\texttt{mean_diff} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta_j}{\texttt{n_snps}}\), mean difference between the standardised beta, predicted from P-values, and the observed beta. The difference should be very close to zero if trait has a variance of 1.
    • \(\widehat{\beta_j^{std}} = \sqrt{\frac{{z}_j^2 / ({z}_j^2 + n -2)}{2 \times {MAF}_j \times (1 - {MAF}_j)}} \times sign({z}_j)\),
    • \({z}_j = \frac{\beta_j}{{se}_j}\),
    • and \(\beta_j\) is the reported effect size.
  • ratio_diff: \(\texttt{ratio_diff} = |\frac{\texttt{mean_diff}}{\texttt{mean_diff2}}|\), absolute ratio between the mean of diff and the mean of diff2 (expected difference between the standardised beta predicted from P-values, and the standardised beta derived from the observed beta divided by the predicted SD; NOT reported). The ratio should be close to 1. If different from 1, then implies that the betas are not in a standard deviation scale.
    • \(\texttt{mean_diff2} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta^{\prime}_j}{\texttt{n_snps}}\)
    • \(\beta^{\prime}_j = \frac{\beta_j}{\widehat{\texttt{sd2}}_{y}}\)
  • sd_y_est1: The standard deviation for the trait inferred from the reported sample size, median standard errors for the SNP-trait assocations and SNP variances.
    • \(\widehat{\texttt{sd1}}_{y} = \frac{\sqrt{n} \times median({se}_j)}{C}\),
    • \(C = median(\frac{1}{\sqrt{2 \times {MAF}_j \times (1 - {MAF}_j)}})\),
    • and \({se}_j\) is the reported standard error.
  • sd_y_est2: The standard deviation for the trait inferred from the reported sample size, Z statistics for the SNP-trait effects (beta/se) and allele frequency.
    • \(\widehat{\texttt{sd2}}_{y} = median(\widehat{sd_j})\),
    • \(\widehat{sd_j} = \frac{\beta_j}{\widehat{\beta_j^{std}}}\),

r2 metrics

Sum of variance explained, calculated from the clumped top hits sample.

  • r2_sum<*>: r2 statistics under various assumptions
    • 1: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var1}}}\), \(\texttt{var1} = 1\).
    • 2: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var2}}}\), \(\texttt{var2} = {\widehat{\texttt{sd1}}_{y}}^2\),
    • 3: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var3}}}\), \(\texttt{var3} = {\widehat{\texttt{sd2}}_{y}}^2\),
    • 4: \(r^2 = \sum_j{\frac{F_j}{F_j + n - 2}}\), \(F = \frac{\beta_j^2}{{se}_j^2}\).

LDSC metrics

Metrics from LD regression

  • ldsc_nsnp_merge_refpanel_ld: Number of remaining SNPs after merging with reference panel LD.
  • ldsc_nsnp_merge_regression_ld: Number of remaining SNPs after merging with regression SNP LD.
  • ldsc_observed_scale_h2_{beta,se} Coefficient value and SE for total observed scale h2.
  • ldsc_intercept_{beta,se}: Coefficient value and SE for intercept. Intercept is expected to be 1.
  • ldsc_lambda_gc: Lambda GC statistics.
  • ldsc_mean_chisq: Mean \(\chi^2\) statistics.
  • ldsc_ratio: \(\frac{\texttt{ldsc_intercept_beta} - 1}{\texttt{ldsc_mean_chisq} - 1}\), the proportion of the inflation in the mean \(\chi^2\) that the LD Score regression intercepts ascribes to causes other than polygenic heritability. The value of ratio should be close to zero, though in practice values of 0.1-0.2 are not uncommon, probably due to sample/reference LD Score mismatch or model misspecification (e.g., low LD variants have slightly higher \(h^2\) per SNP).

Flags

When a metric needs attention, the flag should return TRUE.

  • af_correlation: abs(af_correlation) < 0.7.
  • inflation_factor: inflation_factor > 1.2.
  • n: n (max reported sample size) < 10000.
  • is_snpid_non_unique: NOT is_snpid_unique.
  • mean_EFFECT_nonfinite: mean(EFFECT) is NA, NaN, or Inf.
  • mean_EFFECT_05: abs(mean(EFFECT)) > 0.5.
  • mean_EFFECT_01: abs(mean(EFFECT)) > 0.1.
  • mean_chisq: ldsc_mean_chisq > 1.3 or ldsc_mean_chisq < 0.7.
  • n_p_sig: n_p_sig > 1000.
  • miss_<*>: n_miss_<*> / n_snps > 0.01.
  • ldsc_ratio: ldsc_ratio > 0.5
  • ldsc_intercept_beta: ldsc_intercept_beta > 1.5
  • n_clumped_hits: n_clumped_hits > 1000
  • r2_sum<*>: r2_sum<*> > 0.5

Plots

  • Manhattan plot
    • Red line: \(-log_{10}^{5 \times 10^{-8}}\)
    • Blue line: \(-log_{10}^{5 \times 10^{-5}}\)
  • QQ plot
  • AF plot
  • P-Z plot
  • beta_std plot: Scatter plot between \(\widehat{\beta_j^{std}}\) and \(\beta_j\)

Diagnostics

Details

Summary stats

skim_type skim_variable n_missing complete_rate character.min character.max character.empty character.n_unique character.whitespace logical.mean logical.count numeric.mean numeric.sd numeric.p0 numeric.p25 numeric.p50 numeric.p75 numeric.p100 numeric.hist
character ID 0 1.0000000 3 58 0 7263611 0 NA NA NA NA NA NA NA NA NA NA
character REF 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
character ALT 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
logical N 7268332 0.0000000 NA NA NA NA NA NaN : NA NA NA NA NA NA NA NA
numeric CHROM 0 1.0000000 NA NA NA NA NA NA NA 8.663431e+00 5.763791e+00 1.0000000 4.000000e+00 8.000000e+00 1.300000e+01 2.200000e+01 ▇▅▅▂▂
numeric POS 0 1.0000000 NA NA NA NA NA NA NA 7.863562e+07 5.645057e+07 828.0000000 3.216148e+07 6.905814e+07 1.145200e+08 2.492385e+08 ▇▆▅▂▁
numeric EFFECT 0 1.0000000 NA NA NA NA NA NA NA 6.100000e-06 1.107900e-03 -0.0108401 -5.738000e-04 3.800000e-06 5.805000e-04 1.108150e-02 ▁▁▇▁▁
numeric SE 0 1.0000000 NA NA NA NA NA NA NA 9.415000e-04 4.733000e-04 0.0004961 5.639000e-04 7.404000e-04 1.197200e-03 5.380200e-03 ▇▂▁▁▁
numeric PVAL 0 1.0000000 NA NA NA NA NA NA NA 4.797980e-01 2.942113e-01 0.0000000 2.200002e-01 4.700002e-01 7.400005e-01 1.000000e+00 ▇▇▇▆▇
numeric PVAL_ztest 0 1.0000000 NA NA NA NA NA NA NA 4.797991e-01 2.941839e-01 0.0000000 2.193126e-01 4.729102e-01 7.353358e-01 9.999995e-01 ▇▇▇▆▆
numeric AF 0 1.0000000 NA NA NA NA NA NA NA 2.672068e-01 2.604552e-01 0.0138270 5.215300e-02 1.672980e-01 4.205502e-01 9.861730e-01 ▇▂▂▁▁
numeric AF_reference 67125 0.9907647 NA NA NA NA NA NA NA 2.658076e-01 2.523502e-01 0.0000000 5.870610e-02 1.801120e-01 4.139380e-01 1.000000e+00 ▇▃▂▁▁

Head and tail

CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
1 49298 rs200943160 T C -0.0001541 0.0009135 0.8700001 0.8660217 0.623799 0.7821490 NA
1 54676 rs2462492 C T 0.0007995 0.0009041 0.3800004 0.3765195 0.400475 NA NA
1 86028 rs114608975 T C -0.0016762 0.0014455 0.2500000 0.2461940 0.103585 0.0277556 NA
1 91536 rs6702460 G T 0.0021134 0.0008905 0.0179999 0.0176298 0.456972 0.4207270 NA
1 234313 rs8179466 C T 0.0022226 0.0017570 0.2099999 0.2058790 0.074513 NA NA
1 534192 rs6680723 C T -0.0008602 0.0010176 0.4000000 0.3979298 0.240941 NA NA
1 546697 rs12025928 A G 0.0003800 0.0012684 0.7600007 0.7644881 0.913389 NA NA
1 693731 rs12238997 A G 0.0001967 0.0008528 0.8200001 0.8175498 0.116271 0.1417730 NA
1 705882 rs72631875 G A -0.0001290 0.0012492 0.9199999 0.9177379 0.067339 0.0315495 NA
1 706368 rs55727773 A G -0.0006294 0.0006318 0.3200000 0.3191586 0.515822 0.2751600 NA
CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
22 51219006 rs28729663 G A -0.0006835 0.0007621 0.3700002 0.3697781 0.137913 0.2052720 NA
22 51219387 rs9616832 T C -0.0006961 0.0009890 0.4799997 0.4815486 0.073759 0.0654952 NA
22 51219704 rs147475742 G A -0.0013992 0.0013257 0.2900000 0.2912223 0.041951 0.0473243 NA
22 51221190 rs369304721 G A -0.0010509 0.0013229 0.4299995 0.4269836 0.049740 NA NA
22 51221731 rs115055839 T C -0.0006039 0.0009896 0.5400003 0.5416938 0.073254 0.0625000 NA
22 51222100 rs114553188 G T -0.0008119 0.0011655 0.4899999 0.4860747 0.054398 0.0880591 NA
22 51223637 rs375798137 G A -0.0007962 0.0011712 0.5000000 0.4966037 0.054028 0.0788738 NA
22 51229805 rs9616985 T C -0.0006777 0.0009932 0.5000000 0.4950336 0.073086 0.0730831 NA
22 51232488 rs376461333 A G 0.0009257 0.0023394 0.6899999 0.6923140 0.020022 NA NA
22 51237063 rs3896457 T C 0.0000210 0.0006076 0.9699999 0.9724224 0.298155 0.2050720 NA

bcf preview

1   49298   rs10399793  T   C   .   PASS    AF=0.623799 ES:SE:LP:AF:ID  -0.000154114:0.000913464:0.0604807:0.623799:rs10399793
1   54676   rs2462492   C   T   .   PASS    AF=0.400475 ES:SE:LP:AF:ID  0.000799493:0.000904069:0.420216:0.400475:rs2462492
1   86028   rs114608975 T   C   .   PASS    AF=0.103585 ES:SE:LP:AF:ID  -0.00167623:0.00144547:0.60206:0.103585:rs114608975
1   91536   rs6702460   G   T   .   PASS    AF=0.456972 ES:SE:LP:AF:ID  0.0021134:0.000890489:1.74473:0.456972:rs6702460
1   234313  rs8179466   C   T   .   PASS    AF=0.074513 ES:SE:LP:AF:ID  0.00222258:0.00175701:0.677781:0.074513:rs8179466
1   534192  rs6680723   C   T   .   PASS    AF=0.240941 ES:SE:LP:AF:ID  -0.000860219:0.00101762:0.39794:0.240941:rs6680723
1   546697  rs12025928  A   G   .   PASS    AF=0.913389 ES:SE:LP:AF:ID  0.000379997:0.00126838:0.119186:0.913389:rs12025928
1   693731  rs12238997  A   G   .   PASS    AF=0.116271 ES:SE:LP:AF:ID  0.000196731:0.000852766:0.0861861:0.116271:rs12238997
1   705882  rs72631875  G   A   .   PASS    AF=0.067339 ES:SE:LP:AF:ID  -0.000129025:0.00124923:0.0362122:0.067339:rs72631875
1   706368  rs12029736  A   G   .   PASS    AF=0.515822 ES:SE:LP:AF:ID  -0.000629367:0.000631775:0.49485:0.515822:rs12029736
1   714596  rs149887893 T   C   .   PASS    AF=0.033003 ES:SE:LP:AF:ID  0.000488876:0.00159216:0.119186:0.033003:rs149887893
1   715265  rs12184267  C   T   .   PASS    AF=0.036631 ES:SE:LP:AF:ID  0.000149932:0.00144584:0.0362122:0.036631:rs12184267
1   715367  rs12184277  A   G   .   PASS    AF=0.03674  ES:SE:LP:AF:ID  0.000138324:0.00144055:0.0362122:0.03674:rs12184277
1   717485  rs12184279  C   A   .   PASS    AF=0.036443 ES:SE:LP:AF:ID  0.0001087:0.00145092:0.0268721:0.036443:rs12184279
1   717587  rs144155419 G   A   .   PASS    AF=0.016438 ES:SE:LP:AF:ID  -0.000380589:0.00223227:0.0655015:0.016438:rs144155419
1   720381  rs116801199 G   T   .   PASS    AF=0.036981 ES:SE:LP:AF:ID  0.000205627:0.00143482:0.05061:0.036981:rs116801199
1   721290  rs12565286  G   C   .   PASS    AF=0.037078 ES:SE:LP:AF:ID  0.000265852:0.0014299:0.0705811:0.037078:rs12565286
1   722670  rs116030099 T   C   .   PASS    AF=0.101236 ES:SE:LP:AF:ID  -0.000229519:0.0010419:0.0809219:0.101236:rs116030099
1   723891  rs2977670   G   C   .   PASS    AF=0.959105 ES:SE:LP:AF:ID  -0.000592916:0.00137947:0.173925:0.959105:rs2977670
1   724849  rs12126395  C   A   .   PASS    AF=0.031481 ES:SE:LP:AF:ID  -0.000876452:0.00250117:0.136677:0.031481:rs12126395
1   725060  rs865924913 A   T   .   PASS    AF=0.053294 ES:SE:LP:AF:ID  -0.00253094:0.00198956:0.69897:0.053294:rs865924913
1   726794  rs28454925  C   G   .   PASS    AF=0.036593 ES:SE:LP:AF:ID  0.000223812:0.00143924:0.0555173:0.036593:rs28454925
1   729632  rs116720794 C   T   .   PASS    AF=0.036906 ES:SE:LP:AF:ID  -7.69899e-05:0.00142624:0.0177288:0.036906:rs116720794
1   729679  rs4951859   C   G   .   PASS    AF=0.84324  ES:SE:LP:AF:ID  -0.000167572:0.00073888:0.0861861:0.84324:rs4951859
1   730087  rs148120343 T   C   .   PASS    AF=0.055894 ES:SE:LP:AF:ID  0.0014184:0.0011966:0.619789:0.055894:rs148120343
1   731718  rs58276399  T   C   .   PASS    AF=0.122263 ES:SE:LP:AF:ID  0.000302627:0.000808872:0.148742:0.122263:rs58276399
1   732989  rs369030935 C   T   .   PASS    AF=0.025717 ES:SE:LP:AF:ID  -0.00139756:0.00198774:0.318759:0.025717:rs369030935
1   734349  rs141242758 T   C   .   PASS    AF=0.121509 ES:SE:LP:AF:ID  0.000305708:0.000809213:0.148742:0.121509:rs141242758
1   736289  rs79010578  T   A   .   PASS    AF=0.132307 ES:SE:LP:AF:ID  0.000255944:0.000797554:0.124939:0.132307:rs79010578
1   752478  rs146277091 G   A   .   PASS    AF=0.036832 ES:SE:LP:AF:ID  0.000238392:0.00141159:0.0604807:0.036832:rs146277091
1   752566  rs3094315   G   A   .   PASS    AF=0.838964 ES:SE:LP:AF:ID  -0.000574586:0.00071564:0.376751:0.838964:rs3094315
1   752721  rs3131972   A   G   .   PASS    AF=0.838589 ES:SE:LP:AF:ID  -0.000591859:0.000714829:0.387216:0.838589:rs3131972
1   753405  rs3115860   C   A   .   PASS    AF=0.869839 ES:SE:LP:AF:ID  -0.000739461:0.000767233:0.468521:0.869839:rs3115860
1   753541  rs2073813   G   A   .   PASS    AF=0.129815 ES:SE:LP:AF:ID  0.000692772:0.000768782:0.431798:0.129815:rs2073813
1   754063  rs12184312  G   T   .   PASS    AF=0.037333 ES:SE:LP:AF:ID  0.0002947:0.00138789:0.0809219:0.037333:rs12184312
1   754105  rs12184325  C   T   .   PASS    AF=0.037575 ES:SE:LP:AF:ID  0.000274411:0.00137917:0.0757207:0.037575:rs12184325
1   754182  rs3131969   A   G   .   PASS    AF=0.86918  ES:SE:LP:AF:ID  -0.000733668:0.0007657:0.468521:0.86918:rs3131969
1   754192  rs3131968   A   G   .   PASS    AF=0.869277 ES:SE:LP:AF:ID  -0.000748977:0.000765996:0.481486:0.869277:rs3131968
1   754211  rs12184313  G   A   .   PASS    AF=0.037534 ES:SE:LP:AF:ID  0.000268376:0.00138507:0.0705811:0.037534:rs12184313
1   754334  rs3131967   T   C   .   PASS    AF=0.869181 ES:SE:LP:AF:ID  -0.000749215:0.000765685:0.481486:0.869181:rs3131967
1   754503  rs3115859   G   A   .   PASS    AF=0.83804  ES:SE:LP:AF:ID  -0.00064364:0.000712839:0.431798:0.83804:rs3115859
1   754629  rs10454459  A   G   .   PASS    AF=0.037544 ES:SE:LP:AF:ID  0.000249403:0.00138708:0.0655015:0.037544:rs10454459
1   754964  rs3131966   C   T   .   PASS    AF=0.838674 ES:SE:LP:AF:ID  -0.000605519:0.000714857:0.39794:0.838674:rs3131966
1   755775  rs3131965   A   G   .   PASS    AF=0.839784 ES:SE:LP:AF:ID  -0.000713722:0.000724515:0.49485:0.839784:rs3131965
1   755890  rs3115858   A   T   .   PASS    AF=0.869466 ES:SE:LP:AF:ID  -0.000800523:0.000764849:0.522879:0.869466:rs3115858
1   756604  rs3131962   A   G   .   PASS    AF=0.869014 ES:SE:LP:AF:ID  -0.000793934:0.000762926:0.522879:0.869014:rs3131962
1   757640  rs3115853   G   A   .   PASS    AF=0.86796  ES:SE:LP:AF:ID  -0.000878583:0.000761424:0.60206:0.86796:rs3115853
1   757734  rs4951929   C   T   .   PASS    AF=0.869158 ES:SE:LP:AF:ID  -0.000814124:0.000763552:0.537602:0.869158:rs4951929
1   757936  rs4951862   C   A   .   PASS    AF=0.869166 ES:SE:LP:AF:ID  -0.00081284:0.00076361:0.537602:0.869166:rs4951862
1   758144  rs3131956   A   G   .   PASS    AF=0.869174 ES:SE:LP:AF:ID  -0.000816445:0.000763628:0.552842:0.869174:rs3131956