Summary

Summary {data-width=650}

Manhattan plot

manhattan_plot

manhattan_plot

QQ plot

qq_plot

qq_plot

AF plot

af_plot

af_plot

P-Z plot

pz_plot

pz_plot

beta_std plot

beta_std_plot

beta_std_plot

Metadata

{
    "fileformat": "VCFv4.2",
    "FILTER": "<ID=PASS,Description=\"All filters passed\">",
    "INFO": "<ID=AF,Number=A,Type=Float,Description=\"Allele Frequency\">",
    "FORMAT": "<ID=ES,Number=A,Type=Float,Description=\"Effect size estimate relative to the alternative allele\">",
    "FORMAT.1": "<ID=SE,Number=A,Type=Float,Description=\"Standard error of effect size estimate\">",
    "FORMAT.2": "<ID=LP,Number=A,Type=Float,Description=\"-log10 p-value for effect estimate\">",
    "FORMAT.3": "<ID=AF,Number=A,Type=Float,Description=\"Alternate allele frequency in the association study\">",
    "FORMAT.4": "<ID=SS,Number=A,Type=Float,Description=\"Sample size used to estimate genetic effect\">",
    "FORMAT.5": "<ID=EZ,Number=A,Type=Float,Description=\"Z-score provided if it was used to derive the EFFECT and SE fields\">",
    "FORMAT.6": "<ID=SI,Number=A,Type=Float,Description=\"Accuracy score of summary data imputation\">",
    "FORMAT.7": "<ID=NC,Number=A,Type=Float,Description=\"Number of cases used to estimate genetic effect\">",
    "FORMAT.8": "<ID=ID,Number=1,Type=String,Description=\"Study variant identifier\">",
    "META": "<ID=TotalVariants,Number=1,Type=Integer,Description=\"Total number of variants in input\">",
    "META.1": "<ID=VariantsNotRead,Number=1,Type=Integer,Description=\"Number of variants that could not be read\">",
    "META.2": "<ID=HarmonisedVariants,Number=1,Type=Integer,Description=\"Total number of harmonised variants\">",
    "META.3": "<ID=VariantsNotHarmonised,Number=1,Type=Integer,Description=\"Total number of variants that could not be harmonised\">",
    "META.4": "<ID=SwitchedAlleles,Number=1,Type=Integer,Description=\"Total number of variants strand switched\">",
    "META.5": "<ID=TotalControls,Number=1,Type=Integer,Description=\"Total number of controls in the association study\">",
    "META.6": "<ID=TotalCases,Number=1,Type=Integer,Description=\"Total number of cases in the association study\">",
    "META.7": "<ID=StudyType,Number=1,Type=String,Description=\"Type of GWAS study [Continuous or CaseControl]\">",
    "SAMPLE": "<ID=UKB-b:11495,TotalVariants=9851866,VariantsNotRead=0,HarmonisedVariants=9851866,VariantsNotHarmonised=0,SwitchedAlleles=9851866,TotalControls=224132,TotalCases=236244,StudyType=CaseControl>",
    "contig": "<ID=1,length=249250621,assembly=GRCh37>",
    "contig.1": "<ID=2,length=243199373,assembly=GRCh37>",
    "contig.2": "<ID=3,length=198022430,assembly=GRCh37>",
    "contig.3": "<ID=4,length=191154276,assembly=GRCh37>",
    "contig.4": "<ID=5,length=180915260,assembly=GRCh37>",
    "contig.5": "<ID=6,length=171115067,assembly=GRCh37>",
    "contig.6": "<ID=7,length=159138663,assembly=GRCh37>",
    "contig.7": "<ID=8,length=146364022,assembly=GRCh37>",
    "contig.8": "<ID=9,length=141213431,assembly=GRCh37>",
    "contig.9": "<ID=10,length=135534747,assembly=GRCh37>",
    "contig.10": "<ID=11,length=135006516,assembly=GRCh37>",
    "contig.11": "<ID=12,length=133851895,assembly=GRCh37>",
    "contig.12": "<ID=13,length=115169878,assembly=GRCh37>",
    "contig.13": "<ID=14,length=107349540,assembly=GRCh37>",
    "contig.14": "<ID=15,length=102531392,assembly=GRCh37>",
    "contig.15": "<ID=16,length=90354753,assembly=GRCh37>",
    "contig.16": "<ID=17,length=81195210,assembly=GRCh37>",
    "contig.17": "<ID=18,length=78077248,assembly=GRCh37>",
    "contig.18": "<ID=19,length=59128983,assembly=GRCh37>",
    "contig.19": "<ID=20,length=63025520,assembly=GRCh37>",
    "contig.20": "<ID=21,length=48129895,assembly=GRCh37>",
    "contig.21": "<ID=22,length=51304566,assembly=GRCh37>",
    "contig.22": "<ID=X,length=155270560,assembly=GRCh37>",
    "contig.23": "<ID=Y,length=59373566,assembly=GRCh37>",
    "contig.24": "<ID=MT,length=16569,assembly=GRCh37>",
    "contig.25": "<ID=GL000207.1,length=4262,assembly=GRCh37>",
    "contig.26": "<ID=GL000226.1,length=15008,assembly=GRCh37>",
    "contig.27": "<ID=GL000229.1,length=19913,assembly=GRCh37>",
    "contig.28": "<ID=GL000231.1,length=27386,assembly=GRCh37>",
    "contig.29": "<ID=GL000210.1,length=27682,assembly=GRCh37>",
    "contig.30": "<ID=GL000239.1,length=33824,assembly=GRCh37>",
    "contig.31": "<ID=GL000235.1,length=34474,assembly=GRCh37>",
    "contig.32": "<ID=GL000201.1,length=36148,assembly=GRCh37>",
    "contig.33": "<ID=GL000247.1,length=36422,assembly=GRCh37>",
    "contig.34": "<ID=GL000245.1,length=36651,assembly=GRCh37>",
    "contig.35": "<ID=GL000197.1,length=37175,assembly=GRCh37>",
    "contig.36": "<ID=GL000203.1,length=37498,assembly=GRCh37>",
    "contig.37": "<ID=GL000246.1,length=38154,assembly=GRCh37>",
    "contig.38": "<ID=GL000249.1,length=38502,assembly=GRCh37>",
    "contig.39": "<ID=GL000196.1,length=38914,assembly=GRCh37>",
    "contig.40": "<ID=GL000248.1,length=39786,assembly=GRCh37>",
    "contig.41": "<ID=GL000244.1,length=39929,assembly=GRCh37>",
    "contig.42": "<ID=GL000238.1,length=39939,assembly=GRCh37>",
    "contig.43": "<ID=GL000202.1,length=40103,assembly=GRCh37>",
    "contig.44": "<ID=GL000234.1,length=40531,assembly=GRCh37>",
    "contig.45": "<ID=GL000232.1,length=40652,assembly=GRCh37>",
    "contig.46": "<ID=GL000206.1,length=41001,assembly=GRCh37>",
    "contig.47": "<ID=GL000240.1,length=41933,assembly=GRCh37>",
    "contig.48": "<ID=GL000236.1,length=41934,assembly=GRCh37>",
    "contig.49": "<ID=GL000241.1,length=42152,assembly=GRCh37>",
    "contig.50": "<ID=GL000243.1,length=43341,assembly=GRCh37>",
    "contig.51": "<ID=GL000242.1,length=43523,assembly=GRCh37>",
    "contig.52": "<ID=GL000230.1,length=43691,assembly=GRCh37>",
    "contig.53": "<ID=GL000237.1,length=45867,assembly=GRCh37>",
    "contig.54": "<ID=GL000233.1,length=45941,assembly=GRCh37>",
    "contig.55": "<ID=GL000204.1,length=81310,assembly=GRCh37>",
    "contig.56": "<ID=GL000198.1,length=90085,assembly=GRCh37>",
    "contig.57": "<ID=GL000208.1,length=92689,assembly=GRCh37>",
    "contig.58": "<ID=GL000191.1,length=106433,assembly=GRCh37>",
    "contig.59": "<ID=GL000227.1,length=128374,assembly=GRCh37>",
    "contig.60": "<ID=GL000228.1,length=129120,assembly=GRCh37>",
    "contig.61": "<ID=GL000214.1,length=137718,assembly=GRCh37>",
    "contig.62": "<ID=GL000221.1,length=155397,assembly=GRCh37>",
    "contig.63": "<ID=GL000209.1,length=159169,assembly=GRCh37>",
    "contig.64": "<ID=GL000218.1,length=161147,assembly=GRCh37>",
    "contig.65": "<ID=GL000220.1,length=161802,assembly=GRCh37>",
    "contig.66": "<ID=GL000213.1,length=164239,assembly=GRCh37>",
    "contig.67": "<ID=GL000211.1,length=166566,assembly=GRCh37>",
    "contig.68": "<ID=GL000199.1,length=169874,assembly=GRCh37>",
    "contig.69": "<ID=GL000217.1,length=172149,assembly=GRCh37>",
    "contig.70": "<ID=GL000216.1,length=172294,assembly=GRCh37>",
    "contig.71": "<ID=GL000215.1,length=172545,assembly=GRCh37>",
    "contig.72": "<ID=GL000205.1,length=174588,assembly=GRCh37>",
    "contig.73": "<ID=GL000219.1,length=179198,assembly=GRCh37>",
    "contig.74": "<ID=GL000224.1,length=179693,assembly=GRCh37>",
    "contig.75": "<ID=GL000223.1,length=180455,assembly=GRCh37>",
    "contig.76": "<ID=GL000195.1,length=182896,assembly=GRCh37>",
    "contig.77": "<ID=GL000212.1,length=186858,assembly=GRCh37>",
    "contig.78": "<ID=GL000222.1,length=186861,assembly=GRCh37>",
    "contig.79": "<ID=GL000200.1,length=187035,assembly=GRCh37>",
    "contig.80": "<ID=GL000193.1,length=189789,assembly=GRCh37>",
    "contig.81": "<ID=GL000194.1,length=191469,assembly=GRCh37>",
    "contig.82": "<ID=GL000225.1,length=211173,assembly=GRCh37>",
    "contig.83": "<ID=GL000192.1,length=547496,assembly=GRCh37>",
    "gwas_harmonisation_command": "--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/vcf_09_19b/bgzip_vcf/data.batch_6164_4.vcf.gz --id UKB-b:11495 --data /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/raw-output/data.batch_6164_4.txt.gz --cohort_cases 236244 --cohort_controls 224132 --ref /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/human_g1k_v37.fasta --json /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/ukb_gwas.json; 1.1.1",
    "file_date": "2019-09-13T02:00:38.733542",
    "bcftools_viewVersion": "1.9-74-g6af271c+htslib-1.9-64-g226b4a8",
    "bcftools_viewCommand": "view -T ^/mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-11495/mac_discard.txt -Oz /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-11495/UKB-b-11495_raw.vcf.gz; Date=Thu Oct 17 12:25:33 2019",
    "bcftools_viewCommand.1": "view -h /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukb-b-11495/ukb-b-11495.vcf.gz; Date=Sun May 10 09:44:02 2020"
}

LDSC

*********************************************************************
* LD Score Regression (LDSC)
* Version 1.0.1
* (C) 2014-2019 Brendan Bulik-Sullivan and Hilary Finucane
* Broad Institute of MIT and Harvard / MIT Department of Mathematics
* GNU General Public License v3
*********************************************************************
Call: 
./ldsc.py \
--h2 /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-11495/UKB-b-11495_data.vcf.gz \
--ref-ld-chr ../reference/eur_w_ld_chr/ \
--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-11495/ldsc.txt \
--w-ld-chr ../reference/eur_w_ld_chr/ 

Beginning analysis at Thu Oct 17 14:40:52 2019
Reading summary statistics from /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-11495/UKB-b-11495_data.vcf.gz ...
Read summary statistics for 9606172 SNPs.
Dropped 12497 SNPs with duplicated rs numbers.
Reading reference panel LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read reference panel LD Scores for 1290028 SNPs.
Removing partitioned LD Scores with zero variance.
Reading regression weight LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read regression weight LD Scores for 1290028 SNPs.
After merging with reference panel LD, 1288600 SNPs remain.
After merging with regression SNP LD, 1288600 SNPs remain.
Using two-step estimator with cutoff at 30.
Total Observed scale h2: 0.0377 (0.0018)
Lambda GC: 1.358
Mean Chi^2: 1.4088
Intercept: 1.069 (0.0083)
Ratio: 0.1687 (0.0204)
Analysis finished at Thu Oct 17 14:42:44 2019
Total time elapsed: 1.0m:52.2s

QC metrics

Metrics

Metrics

{
    "af_correlation": 0.9493,
    "inflation_factor": 1.2544,
    "mean_EFFECT": -0.0001,
    "n": "-Inf",
    "n_snps": 9851866,
    "n_clumped_hits": 13,
    "n_p_sig": 753,
    "n_mono": 0,
    "n_ns": 0,
    "n_mac": 0,
    "is_snpid_unique": true,
    "n_miss_EFFECT": 0,
    "n_miss_SE": 0,
    "n_miss_PVAL": 0,
    "n_miss_AF": 0,
    "n_miss_AF_reference": 147663,
    "n_est": "NA",
    "ratio_se_n": "NA",
    "mean_diff": "NaN",
    "ratio_diff": "NaN",
    "sd_y_est1": "NaN",
    "sd_y_est2": "NA",
    "r2_sum1": 0,
    "r2_sum2": 0,
    "r2_sum3": 0,
    "r2_sum4": 0,
    "ldsc_nsnp_merge_refpanel_ld": 1288600,
    "ldsc_nsnp_merge_regression_ld": 1288600,
    "ldsc_observed_scale_h2_beta": 0.0377,
    "ldsc_observed_scale_h2_se": 0.0018,
    "ldsc_intercept_beta": 1.069,
    "ldsc_intercept_se": 0.0083,
    "ldsc_lambda_gc": 1.358,
    "ldsc_mean_chisq": 1.4088,
    "ldsc_ratio": 0.1688
}

Flags

name value
af_correlation FALSE
inflation_factor TRUE
n TRUE
is_snpid_non_unique FALSE
mean_EFFECT_nonfinite FALSE
mean_EFFECT_05 FALSE
mean_EFFECT_01 FALSE
mean_chisq TRUE
n_p_sig FALSE
miss_EFFECT FALSE
miss_SE FALSE
miss_PVAL FALSE
ldsc_ratio FALSE
ldsc_intercept_beta FALSE
n_clumped_hits FALSE
r2_sum1 FALSE
r2_sum2 FALSE
r2_sum3 FALSE
r2_sum4 FALSE

Definitions

General metrics

  • af_correlation: Correlation coefficient between AF and AF_reference.
  • inflation_factor (lambda): Genomic inflation factor.
  • mean_EFFECT: Mean of EFFECT size.
  • n: Maximum value of reported sample size across all SNPs, \(n\).
  • n_clumped_hits: Number of clumped hits.
  • n_snps: Number of SNPs
  • n_p_sig: Number of SNPs with pvalue below 5e-8.
  • n_mono: Number of monomorphic (MAF == 1 or MAF == 0) SNPs.
  • n_ns: Number of SNPs with nonsense values:
    • alleles other than A, C, G or T.
    • P-values < 0 or > 1.
    • negative or infinite standard errors (<= 0 or = Infinity).
    • infinite beta estimates or allele frequencies < 0 or > 1.
  • n_mac: Number of cases where MAC (\(2 \times N \times MAF\)) is less than 6.
  • is_snpid_unique: true if the combination of ID REF ALT is unique and therefore no duplication in snpid.
  • n_miss_<*>: Number of NA observations for <*> column.

se_n metrics

  • n_est: Estimated sample size value, \(\widehat{n}\).
  • ratio_se_n: \(\texttt{ratio_se_n} = \frac{\sqrt{\widehat{n}}}{\sqrt{n}}\). We expect ratio_se_n to be 1. When it is not 1, it implies that the trait did not have a variance of 1, the reported sample size is wrong, or that the SNP-level effective sample sizes differ markedly from the reported sample size.
  • mean_diff: \(\texttt{mean_diff} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta_j}{\texttt{n_snps}}\), mean difference between the standardised beta, predicted from P-values, and the observed beta. The difference should be very close to zero if trait has a variance of 1.
    • \(\widehat{\beta_j^{std}} = \sqrt{\frac{{z}_j^2 / ({z}_j^2 + n -2)}{2 \times {MAF}_j \times (1 - {MAF}_j)}} \times sign({z}_j)\),
    • \({z}_j = \frac{\beta_j}{{se}_j}\),
    • and \(\beta_j\) is the reported effect size.
  • ratio_diff: \(\texttt{ratio_diff} = |\frac{\texttt{mean_diff}}{\texttt{mean_diff2}}|\), absolute ratio between the mean of diff and the mean of diff2 (expected difference between the standardised beta predicted from P-values, and the standardised beta derived from the observed beta divided by the predicted SD; NOT reported). The ratio should be close to 1. If different from 1, then implies that the betas are not in a standard deviation scale.
    • \(\texttt{mean_diff2} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta^{\prime}_j}{\texttt{n_snps}}\)
    • \(\beta^{\prime}_j = \frac{\beta_j}{\widehat{\texttt{sd2}}_{y}}\)
  • sd_y_est1: The standard deviation for the trait inferred from the reported sample size, median standard errors for the SNP-trait assocations and SNP variances.
    • \(\widehat{\texttt{sd1}}_{y} = \frac{\sqrt{n} \times median({se}_j)}{C}\),
    • \(C = median(\frac{1}{\sqrt{2 \times {MAF}_j \times (1 - {MAF}_j)}})\),
    • and \({se}_j\) is the reported standard error.
  • sd_y_est2: The standard deviation for the trait inferred from the reported sample size, Z statistics for the SNP-trait effects (beta/se) and allele frequency.
    • \(\widehat{\texttt{sd2}}_{y} = median(\widehat{sd_j})\),
    • \(\widehat{sd_j} = \frac{\beta_j}{\widehat{\beta_j^{std}}}\),

r2 metrics

Sum of variance explained, calculated from the clumped top hits sample.

  • r2_sum<*>: r2 statistics under various assumptions
    • 1: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var1}}}\), \(\texttt{var1} = 1\).
    • 2: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var2}}}\), \(\texttt{var2} = {\widehat{\texttt{sd1}}_{y}}^2\),
    • 3: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var3}}}\), \(\texttt{var3} = {\widehat{\texttt{sd2}}_{y}}^2\),
    • 4: \(r^2 = \sum_j{\frac{F_j}{F_j + n - 2}}\), \(F = \frac{\beta_j^2}{{se}_j^2}\).

LDSC metrics

Metrics from LD regression

  • ldsc_nsnp_merge_refpanel_ld: Number of remaining SNPs after merging with reference panel LD.
  • ldsc_nsnp_merge_regression_ld: Number of remaining SNPs after merging with regression SNP LD.
  • ldsc_observed_scale_h2_{beta,se} Coefficient value and SE for total observed scale h2.
  • ldsc_intercept_{beta,se}: Coefficient value and SE for intercept. Intercept is expected to be 1.
  • ldsc_lambda_gc: Lambda GC statistics.
  • ldsc_mean_chisq: Mean \(\chi^2\) statistics.
  • ldsc_ratio: \(\frac{\texttt{ldsc_intercept_beta} - 1}{\texttt{ldsc_mean_chisq} - 1}\), the proportion of the inflation in the mean \(\chi^2\) that the LD Score regression intercepts ascribes to causes other than polygenic heritability. The value of ratio should be close to zero, though in practice values of 0.1-0.2 are not uncommon, probably due to sample/reference LD Score mismatch or model misspecification (e.g., low LD variants have slightly higher \(h^2\) per SNP).

Flags

When a metric needs attention, the flag should return TRUE.

  • af_correlation: abs(af_correlation) < 0.7.
  • inflation_factor: inflation_factor > 1.2.
  • n: n (max reported sample size) < 10000.
  • is_snpid_non_unique: NOT is_snpid_unique.
  • mean_EFFECT_nonfinite: mean(EFFECT) is NA, NaN, or Inf.
  • mean_EFFECT_05: abs(mean(EFFECT)) > 0.5.
  • mean_EFFECT_01: abs(mean(EFFECT)) > 0.1.
  • mean_chisq: ldsc_mean_chisq > 1.3 or ldsc_mean_chisq < 0.7.
  • n_p_sig: n_p_sig > 1000.
  • miss_<*>: n_miss_<*> / n_snps > 0.01.
  • ldsc_ratio: ldsc_ratio > 0.5
  • ldsc_intercept_beta: ldsc_intercept_beta > 1.5
  • n_clumped_hits: n_clumped_hits > 1000
  • r2_sum<*>: r2_sum<*> > 0.5

Plots

  • Manhattan plot
    • Red line: \(-log_{10}^{5 \times 10^{-8}}\)
    • Blue line: \(-log_{10}^{5 \times 10^{-5}}\)
  • QQ plot
  • AF plot
  • P-Z plot
  • beta_std plot: Scatter plot between \(\widehat{\beta_j^{std}}\) and \(\beta_j\)

Diagnostics

Details

Summary stats

skim_type skim_variable n_missing complete_rate character.min character.max character.empty character.n_unique character.whitespace logical.mean logical.count numeric.mean numeric.sd numeric.p0 numeric.p25 numeric.p50 numeric.p75 numeric.p100 numeric.hist
character ID 0 1.0000000 3 58 0 9593737 0 NA NA NA NA NA NA NA NA NA NA
character REF 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
character ALT 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
logical N 9606172 0.0000000 NA NA NA NA NA NaN : NA NA NA NA NA NA NA NA
numeric CHROM 0 1.0000000 NA NA NA NA NA NA NA 8.627378e+00 5.750861e+00 1.0000000 4.000000e+00 8.000000e+00 1.300000e+01 2.200000e+01 ▇▅▃▂▂
numeric POS 0 1.0000000 NA NA NA NA NA NA NA 7.883497e+07 5.629804e+07 828.0000000 3.255002e+07 6.943261e+07 1.145670e+08 2.492385e+08 ▇▆▅▂▁
numeric EFFECT 0 1.0000000 NA NA NA NA NA NA NA -1.035000e-04 4.600100e-03 -0.0681020 -1.680800e-03 -3.560000e-05 1.567400e-03 1.412250e-01 ▁▇▁▁▁
numeric SE 0 1.0000000 NA NA NA NA NA NA NA 3.274200e-03 2.840400e-03 0.0010001 1.214500e-03 1.981500e-03 4.429600e-03 5.254380e-02 ▇▁▁▁▁
numeric PVAL 0 1.0000000 NA NA NA NA NA NA NA 4.622804e-01 2.985624e-01 0.0000000 1.900002e-01 4.500005e-01 7.199992e-01 1.000000e+00 ▇▆▆▆▆
numeric PVAL_ztest 0 1.0000000 NA NA NA NA NA NA NA 4.622804e-01 2.985353e-01 0.0000000 1.940116e-01 4.482714e-01 7.209324e-01 9.999998e-01 ▇▆▆▆▆
numeric AF 0 1.0000000 NA NA NA NA NA NA NA 2.078045e-01 2.571183e-01 0.0014820 1.495100e-02 8.410900e-02 3.247162e-01 9.985180e-01 ▇▂▁▁▁
numeric AF_reference 147663 0.9846283 NA NA NA NA NA NA NA 2.099358e-01 2.487790e-01 0.0000000 1.277960e-02 1.044330e-01 3.266770e-01 1.000000e+00 ▇▂▁▁▁

Head and tail

CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
1 49298 rs200943160 T C 0.0039419 0.0018400 0.0320000 0.0321631 0.623782 0.7821490 NA
1 54676 rs2462492 C T -0.0032870 0.0018226 0.0710003 0.0713136 0.400397 NA NA
1 86028 rs114608975 T C -0.0000380 0.0029135 0.9900000 0.9895848 0.103566 0.0277556 NA
1 91536 rs6702460 G T 0.0004718 0.0017946 0.7899998 0.7926239 0.456836 0.4207270 NA
1 234313 rs8179466 C T 0.0006971 0.0035376 0.8400000 0.8437811 0.074517 NA NA
1 534192 rs6680723 C T 0.0034646 0.0020502 0.0909997 0.0910433 0.240936 NA NA
1 546697 rs12025928 A G 0.0010578 0.0025579 0.6800001 0.6792184 0.913502 NA NA
1 693731 rs12238997 A G 0.0009156 0.0017177 0.5900000 0.5940148 0.116349 0.1417730 NA
1 705882 rs72631875 G A -0.0008183 0.0025177 0.7499995 0.7451560 0.067272 0.0315495 NA
1 706368 rs55727773 A G 0.0004051 0.0012725 0.7499995 0.7502064 0.515605 0.2751600 NA
CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
22 51219704 rs147475742 G A 0.0013651 0.0026722 0.6100002 0.6094702 0.041939 0.0473243 NA
22 51219766 rs182321900 C T -0.0034473 0.0124425 0.7800007 0.7817371 0.001938 NA NA
22 51220146 rs868950473 C T -0.0044879 0.0123270 0.7199992 0.7158070 0.001986 NA NA
22 51221190 rs369304721 G A -0.0003779 0.0026680 0.8900000 0.8873748 0.049715 NA NA
22 51221731 rs115055839 T C -0.0000680 0.0019952 0.9699999 0.9728242 0.073221 0.0625000 NA
22 51222100 rs114553188 G T 0.0028872 0.0023485 0.2200002 0.2189221 0.054465 0.0880591 NA
22 51223637 rs375798137 G A 0.0030775 0.0023598 0.1900002 0.1921935 0.054096 0.0788738 NA
22 51229805 rs9616985 T C -0.0000958 0.0020025 0.9599999 0.9618520 0.073053 0.0730831 NA
22 51232488 rs376461333 A G 0.0028835 0.0047150 0.5400003 0.5408268 0.020051 NA NA
22 51237063 rs3896457 T C -0.0006171 0.0012247 0.6100002 0.6143570 0.297957 0.2050720 NA

bcf preview

1   49298   rs10399793  T   C   .   PASS    AF=0.623782 ES:SE:LP:AF:ID  0.00394189:0.00183996:1.49485:0.623782:rs10399793
1   54676   rs2462492   C   T   .   PASS    AF=0.400397 ES:SE:LP:AF:ID  -0.00328696:0.00182257:1.14874:0.400397:rs2462492
1   86028   rs114608975 T   C   .   PASS    AF=0.103566 ES:SE:LP:AF:ID  -3.80323e-05:0.00291348:0.00436481:0.103566:rs114608975
1   91536   rs6702460   G   T   .   PASS    AF=0.456836 ES:SE:LP:AF:ID  0.000471817:0.00179463:0.102373:0.456836:rs6702460
1   234313  rs8179466   C   T   .   PASS    AF=0.074517 ES:SE:LP:AF:ID  0.000697119:0.00353761:0.0757207:0.074517:rs8179466
1   534192  rs6680723   C   T   .   PASS    AF=0.240936 ES:SE:LP:AF:ID  0.00346464:0.00205018:1.04096:0.240936:rs6680723
1   546697  rs12025928  A   G   .   PASS    AF=0.913502 ES:SE:LP:AF:ID  0.00105776:0.00255788:0.167491:0.913502:rs12025928
1   693731  rs12238997  A   G   .   PASS    AF=0.116349 ES:SE:LP:AF:ID  0.000915586:0.00171771:0.229148:0.116349:rs12238997
1   705882  rs72631875  G   A   .   PASS    AF=0.067272 ES:SE:LP:AF:ID  -0.000818339:0.00251771:0.124939:0.067272:rs72631875
1   706368  rs12029736  A   G   .   PASS    AF=0.515605 ES:SE:LP:AF:ID  0.000405135:0.00127254:0.124939:0.515605:rs12029736
1   714596  rs149887893 T   C   .   PASS    AF=0.032986 ES:SE:LP:AF:ID  0.000369329:0.00320928:0.0409586:0.032986:rs149887893
1   715265  rs12184267  C   T   .   PASS    AF=0.036602 ES:SE:LP:AF:ID  -8.48982e-05:0.00291499:0.00877392:0.036602:rs12184267
1   715367  rs12184277  A   G   .   PASS    AF=0.036718 ES:SE:LP:AF:ID  -1.48436e-05:0.00290394:-0:0.036718:rs12184277
1   717485  rs12184279  C   A   .   PASS    AF=0.036419 ES:SE:LP:AF:ID  -6.57423e-06:0.00292478:-0:0.036419:rs12184279
1   717587  rs144155419 G   A   .   PASS    AF=0.016401 ES:SE:LP:AF:ID  -0.000452004:0.00450345:0.0362122:0.016401:rs144155419
1   720381  rs116801199 G   T   .   PASS    AF=0.036957 ES:SE:LP:AF:ID  7.78712e-07:0.00289246:-0:0.036957:rs116801199
1   721290  rs12565286  G   C   .   PASS    AF=0.037053 ES:SE:LP:AF:ID  5.56962e-05:0.00288258:0.00877392:0.037053:rs12565286
1   722670  rs116030099 T   C   .   PASS    AF=0.101242 ES:SE:LP:AF:ID  -0.000820402:0.00209914:0.154902:0.101242:rs116030099
1   723891  rs2977670   G   C   .   PASS    AF=0.959112 ES:SE:LP:AF:ID  0.000968483:0.00277998:0.136677:0.959112:rs2977670
1   724849  rs12126395  C   A   .   PASS    AF=0.031448 ES:SE:LP:AF:ID  -0.00624973:0.00504578:0.657577:0.031448:rs12126395
1   725060  rs865924913 A   T   .   PASS    AF=0.053275 ES:SE:LP:AF:ID  -0.00287008:0.0040125:0.327902:0.053275:rs865924913
1   726794  rs28454925  C   G   .   PASS    AF=0.036574 ES:SE:LP:AF:ID  0.000168956:0.00290108:0.0222764:0.036574:rs28454925
1   729632  rs116720794 C   T   .   PASS    AF=0.036886 ES:SE:LP:AF:ID  -7.07086e-05:0.00287484:0.00877392:0.036886:rs116720794
1   729679  rs4951859   C   G   .   PASS    AF=0.843211 ES:SE:LP:AF:ID  -0.000262332:0.00148881:0.0655015:0.843211:rs4951859
1   730087  rs148120343 T   C   .   PASS    AF=0.055936 ES:SE:LP:AF:ID  -0.000961144:0.00241003:0.161151:0.055936:rs148120343
1   731718  rs58276399  T   C   .   PASS    AF=0.122327 ES:SE:LP:AF:ID  0.000401349:0.00162944:0.091515:0.122327:rs58276399
1   732989  rs369030935 C   T   .   PASS    AF=0.025679 ES:SE:LP:AF:ID  -0.00409834:0.004011:0.508638:0.025679:rs369030935
1   734349  rs141242758 T   C   .   PASS    AF=0.12157  ES:SE:LP:AF:ID  0.000370538:0.00163012:0.0861861:0.12157:rs141242758
1   736289  rs79010578  T   A   .   PASS    AF=0.132338 ES:SE:LP:AF:ID  0.00113665:0.00160683:0.318759:0.132338:rs79010578
1   736689  rs181876450 T   C   .   PASS    AF=0.011143 ES:SE:LP:AF:ID  -0.00423404:0.00583873:0.327902:0.011143:rs181876450
1   740284  rs61770167  C   T   .   PASS    AF=0.005705 ES:SE:LP:AF:ID  0.0109799:0.00753802:0.823909:0.005705:rs61770167
1   742813  rs112573343 C   T   .   PASS    AF=0.002272 ES:SE:LP:AF:ID  -0.00110612:0.0126722:0.0315171:0.002272:rs112573343
1   752478  rs146277091 G   A   .   PASS    AF=0.036802 ES:SE:LP:AF:ID  -0.000259376:0.00284573:0.0315171:0.036802:rs146277091
1   752566  rs3094315   G   A   .   PASS    AF=0.83895  ES:SE:LP:AF:ID  -0.000134079:0.00144178:0.0315171:0.83895:rs3094315
1   752721  rs3131972   A   G   .   PASS    AF=0.838577 ES:SE:LP:AF:ID  -8.48291e-05:0.00144022:0.0222764:0.838577:rs3131972
1   753405  rs3115860   C   A   .   PASS    AF=0.869761 ES:SE:LP:AF:ID  -1.74698e-05:0.00154535:0.00436481:0.869761:rs3115860
1   753541  rs2073813   G   A   .   PASS    AF=0.129886 ES:SE:LP:AF:ID  -0.000201628:0.00154849:0.0457575:0.129886:rs2073813
1   754063  rs12184312  G   T   .   PASS    AF=0.037312 ES:SE:LP:AF:ID  0.000243254:0.00279751:0.0315171:0.037312:rs12184312
1   754105  rs12184325  C   T   .   PASS    AF=0.037556 ES:SE:LP:AF:ID  0.000246447:0.00277983:0.0315171:0.037556:rs12184325
1   754182  rs3131969   A   G   .   PASS    AF=0.869103 ES:SE:LP:AF:ID  -2.78345e-05:0.00154232:0.00436481:0.869103:rs3131969
1   754192  rs3131968   A   G   .   PASS    AF=0.869201 ES:SE:LP:AF:ID  1.00097e-05:0.00154293:0.00436481:0.869201:rs3131968
1   754211  rs12184313  G   A   .   PASS    AF=0.037514 ES:SE:LP:AF:ID  0.000213574:0.00279187:0.0268721:0.037514:rs12184313
1   754334  rs3131967   T   C   .   PASS    AF=0.869106 ES:SE:LP:AF:ID  -4.05116e-05:0.00154229:0.00877392:0.869106:rs3131967
1   754433  rs150578204 G   A   .   PASS    AF=0.005126 ES:SE:LP:AF:ID  -0.00234302:0.0079148:0.113509:0.005126:rs150578204
1   754458  rs142682604 G   T   .   PASS    AF=0.005092 ES:SE:LP:AF:ID  -0.00211253:0.00793558:0.102373:0.005092:rs142682604
1   754503  rs3115859   G   A   .   PASS    AF=0.838032 ES:SE:LP:AF:ID  -0.000276947:0.00143623:0.0705811:0.838032:rs3115859
1   754629  rs10454459  A   G   .   PASS    AF=0.037527 ES:SE:LP:AF:ID  0.000220865:0.00279581:0.0268721:0.037527:rs10454459
1   754964  rs3131966   C   T   .   PASS    AF=0.838663 ES:SE:LP:AF:ID  -0.000302565:0.00144027:0.0809219:0.838663:rs3131966
1   755240  rs181660517 T   G   .   PASS    AF=0.013783 ES:SE:LP:AF:ID  -0.0019592:0.00502536:0.154902:0.013783:rs181660517
1   755435  rs184270342 T   G   .   PASS    AF=0.005546 ES:SE:LP:AF:ID  -0.00581183:0.00775745:0.346787:0.005546:rs184270342