Summary

Summary {data-width=650}

Manhattan plot

manhattan_plot

manhattan_plot

QQ plot

qq_plot

qq_plot

AF plot

af_plot

af_plot

P-Z plot

pz_plot

pz_plot

beta_std plot

beta_std_plot

beta_std_plot

Metadata

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    "gwas_harmonisation_command": "--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/vcf_09_19b/bgzip_vcf/data.batch_5983.vcf.gz --id UKB-b:11361 --data /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/raw-output/data.batch_5983.txt.gz --cohort_controls 68160 --ref /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/human_g1k_v37.fasta --json /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/ukbiobank/ukb_gwas.json; 1.1.1",
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}
 

LDSC

*********************************************************************
* LD Score Regression (LDSC)
* Version 1.0.1
* (C) 2014-2019 Brendan Bulik-Sullivan and Hilary Finucane
* Broad Institute of MIT and Harvard / MIT Department of Mathematics
* GNU General Public License v3
*********************************************************************
Call: 
./ldsc.py \
--h2 /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-11361/UKB-b-11361_data.vcf.gz \
--ref-ld-chr ../reference/eur_w_ld_chr/ \
--out /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-11361/ldsc.txt \
--w-ld-chr ../reference/eur_w_ld_chr/ 

Beginning analysis at Thu Oct 17 14:40:18 2019
Reading summary statistics from /mnt/storage/private/mrcieu/research/scratch/IGD/data/public/UKB-b-11361/UKB-b-11361_data.vcf.gz ...
Read summary statistics for 8692023 SNPs.
Dropped 7559 SNPs with duplicated rs numbers.
Reading reference panel LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read reference panel LD Scores for 1290028 SNPs.
Removing partitioned LD Scores with zero variance.
Reading regression weight LD Score from ../reference/eur_w_ld_chr/[1-22] ...
Read regression weight LD Scores for 1290028 SNPs.
After merging with reference panel LD, 1286052 SNPs remain.
After merging with regression SNP LD, 1286052 SNPs remain.
Using two-step estimator with cutoff at 30.
Total Observed scale h2: 0.0943 (0.01)
Lambda GC: 1.1234
Mean Chi^2: 1.15
Intercept: 1.0247 (0.0082)
Ratio: 0.165 (0.0546)
Analysis finished at Thu Oct 17 14:41:55 2019
Total time elapsed: 1.0m:36.98s

QC metrics

Metrics

Metrics

{
    "af_correlation": 0.9467,
    "inflation_factor": 1.0966,
    "mean_EFFECT": 0.0001,
    "n": "-Inf",
    "n_snps": 9851866,
    "n_clumped_hits": 12,
    "n_p_sig": 594,
    "n_mono": 0,
    "n_ns": 0,
    "n_mac": 0,
    "is_snpid_unique": true,
    "n_miss_EFFECT": 0,
    "n_miss_SE": 0,
    "n_miss_PVAL": 0,
    "n_miss_AF": 0,
    "n_miss_AF_reference": 84342,
    "n_est": "NA",
    "ratio_se_n": "NA",
    "mean_diff": "NaN",
    "ratio_diff": "NaN",
    "sd_y_est1": "NaN",
    "sd_y_est2": "NA",
    "r2_sum1": 0,
    "r2_sum2": 0,
    "r2_sum3": 0,
    "r2_sum4": 0,
    "ldsc_nsnp_merge_refpanel_ld": 1286052,
    "ldsc_nsnp_merge_regression_ld": 1286052,
    "ldsc_observed_scale_h2_beta": 0.0943,
    "ldsc_observed_scale_h2_se": 0.01,
    "ldsc_intercept_beta": 1.0247,
    "ldsc_intercept_se": 0.0082,
    "ldsc_lambda_gc": 1.1234,
    "ldsc_mean_chisq": 1.15,
    "ldsc_ratio": 0.1647
}
 

Flags

name value
af_correlation FALSE
inflation_factor FALSE
n TRUE
is_snpid_non_unique FALSE
mean_EFFECT_nonfinite FALSE
mean_EFFECT_05 FALSE
mean_EFFECT_01 FALSE
mean_chisq FALSE
n_p_sig FALSE
miss_EFFECT FALSE
miss_SE FALSE
miss_PVAL FALSE
ldsc_ratio FALSE
ldsc_intercept_beta FALSE
n_clumped_hits FALSE
r2_sum1 FALSE
r2_sum2 FALSE
r2_sum3 FALSE
r2_sum4 FALSE

Definitions

General metrics

  • af_correlation: Correlation coefficient between AF and AF_reference.
  • inflation_factor (lambda): Genomic inflation factor.
  • mean_EFFECT: Mean of EFFECT size.
  • n: Maximum value of reported sample size across all SNPs, \(n\).
  • n_clumped_hits: Number of clumped hits.
  • n_snps: Number of SNPs
  • n_p_sig: Number of SNPs with pvalue below 5e-8.
  • n_mono: Number of monomorphic (MAF == 1 or MAF == 0) SNPs.
  • n_ns: Number of SNPs with nonsense values:
    • alleles other than A, C, G or T.
    • P-values < 0 or > 1.
    • negative or infinite standard errors (<= 0 or = Infinity).
    • infinite beta estimates or allele frequencies < 0 or > 1.
  • n_mac: Number of cases where MAC (\(2 \times N \times MAF\)) is less than 6.
  • is_snpid_unique: true if the combination of ID REF ALT is unique and therefore no duplication in snpid.
  • n_miss_<*>: Number of NA observations for <*> column.

se_n metrics

  • n_est: Estimated sample size value, \(\widehat{n}\).
  • ratio_se_n: \(\texttt{ratio_se_n} = \frac{\sqrt{\widehat{n}}}{\sqrt{n}}\). We expect ratio_se_n to be 1. When it is not 1, it implies that the trait did not have a variance of 1, the reported sample size is wrong, or that the SNP-level effective sample sizes differ markedly from the reported sample size.
  • mean_diff: \(\texttt{mean_diff} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta_j}{\texttt{n_snps}}\), mean difference between the standardised beta, predicted from P-values, and the observed beta. The difference should be very close to zero if trait has a variance of 1.
    • \(\widehat{\beta_j^{std}} = \sqrt{\frac{{z}_j^2 / ({z}_j^2 + n -2)}{2 \times {MAF}_j \times (1 - {MAF}_j)}} \times sign({z}_j)\),
    • \({z}_j = \frac{\beta_j}{{se}_j}\),
    • and \(\beta_j\) is the reported effect size.
  • ratio_diff: \(\texttt{ratio_diff} = |\frac{\texttt{mean_diff}}{\texttt{mean_diff2}}|\), absolute ratio between the mean of diff and the mean of diff2 (expected difference between the standardised beta predicted from P-values, and the standardised beta derived from the observed beta divided by the predicted SD; NOT reported). The ratio should be close to 1. If different from 1, then implies that the betas are not in a standard deviation scale.
    • \(\texttt{mean_diff2} = \sum_{j} \frac{\widehat{\beta_j^{std}} - \beta^{\prime}_j}{\texttt{n_snps}}\)
    • \(\beta^{\prime}_j = \frac{\beta_j}{\widehat{\texttt{sd2}}_{y}}\)
  • sd_y_est1: The standard deviation for the trait inferred from the reported sample size, median standard errors for the SNP-trait assocations and SNP variances.
    • \(\widehat{\texttt{sd1}}_{y} = \frac{\sqrt{n} \times median({se}_j)}{C}\),
    • \(C = median(\frac{1}{\sqrt{2 \times {MAF}_j \times (1 - {MAF}_j)}})\),
    • and \({se}_j\) is the reported standard error.
  • sd_y_est2: The standard deviation for the trait inferred from the reported sample size, Z statistics for the SNP-trait effects (beta/se) and allele frequency.
    • \(\widehat{\texttt{sd2}}_{y} = median(\widehat{sd_j})\),
    • \(\widehat{sd_j} = \frac{\beta_j}{\widehat{\beta_j^{std}}}\),

r2 metrics

Sum of variance explained, calculated from the clumped top hits sample.

  • r2_sum<*>: r2 statistics under various assumptions
    • 1: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var1}}}\), \(\texttt{var1} = 1\).
    • 2: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var2}}}\), \(\texttt{var2} = {\widehat{\texttt{sd1}}_{y}}^2\),
    • 3: \(r^2 = \sum_j{\frac{2 \times \beta_j^2 \times {MAF}_j \times (1 - {MAF}_j)}{\texttt{var3}}}\), \(\texttt{var3} = {\widehat{\texttt{sd2}}_{y}}^2\),
    • 4: \(r^2 = \sum_j{\frac{F_j}{F_j + n - 2}}\), \(F = \frac{\beta_j^2}{{se}_j^2}\).

LDSC metrics

Metrics from LD regression

  • ldsc_nsnp_merge_refpanel_ld: Number of remaining SNPs after merging with reference panel LD.
  • ldsc_nsnp_merge_regression_ld: Number of remaining SNPs after merging with regression SNP LD.
  • ldsc_observed_scale_h2_{beta,se} Coefficient value and SE for total observed scale h2.
  • ldsc_intercept_{beta,se}: Coefficient value and SE for intercept. Intercept is expected to be 1.
  • ldsc_lambda_gc: Lambda GC statistics.
  • ldsc_mean_chisq: Mean \(\chi^2\) statistics.
  • ldsc_ratio: \(\frac{\texttt{ldsc_intercept_beta} - 1}{\texttt{ldsc_mean_chisq} - 1}\), the proportion of the inflation in the mean \(\chi^2\) that the LD Score regression intercepts ascribes to causes other than polygenic heritability. The value of ratio should be close to zero, though in practice values of 0.1-0.2 are not uncommon, probably due to sample/reference LD Score mismatch or model misspecification (e.g., low LD variants have slightly higher \(h^2\) per SNP).

Flags

When a metric needs attention, the flag should return TRUE.

  • af_correlation: abs(af_correlation) < 0.7.
  • inflation_factor: inflation_factor > 1.2.
  • n: n (max reported sample size) < 10000.
  • is_snpid_non_unique: NOT is_snpid_unique.
  • mean_EFFECT_nonfinite: mean(EFFECT) is NA, NaN, or Inf.
  • mean_EFFECT_05: abs(mean(EFFECT)) > 0.5.
  • mean_EFFECT_01: abs(mean(EFFECT)) > 0.1.
  • mean_chisq: ldsc_mean_chisq > 1.3 or ldsc_mean_chisq < 0.7.
  • n_p_sig: n_p_sig > 1000.
  • miss_<*>: n_miss_<*> / n_snps > 0.01.
  • ldsc_ratio: ldsc_ratio > 0.5
  • ldsc_intercept_beta: ldsc_intercept_beta > 1.5
  • n_clumped_hits: n_clumped_hits > 1000
  • r2_sum<*>: r2_sum<*> > 0.5

Plots

  • Manhattan plot
    • Red line: \(-log_{10}^{5 \times 10^{-8}}\)
    • Blue line: \(-log_{10}^{5 \times 10^{-5}}\)
  • QQ plot
  • AF plot
  • P-Z plot
  • beta_std plot: Scatter plot between \(\widehat{\beta_j^{std}}\) and \(\beta_j\)

Diagnostics

Details

Summary stats

skim_type skim_variable n_missing complete_rate character.min character.max character.empty character.n_unique character.whitespace logical.mean logical.count numeric.mean numeric.sd numeric.p0 numeric.p25 numeric.p50 numeric.p75 numeric.p100 numeric.hist
character ID 0 1.0000000 3 58 0 8684499 0 NA NA NA NA NA NA NA NA NA NA
character REF 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
character ALT 0 1.0000000 1 1 0 4 0 NA NA NA NA NA NA NA NA NA NA
logical N 8692023 0.0000000 NA NA NA NA NA NaN : NA NA NA NA NA NA NA NA
numeric CHROM 0 1.0000000 NA NA NA NA NA NA NA 8.649495e+00 5.760880e+00 1.0000000 4.000000e+00 8.000000e+00 1.300000e+01 2.200000e+01 ▇▅▅▂▂
numeric POS 0 1.0000000 NA NA NA NA NA NA NA 7.877071e+07 5.636025e+07 828.0000000 3.239277e+07 6.929868e+07 1.145662e+08 2.492385e+08 ▇▆▅▂▁
numeric EFFECT 0 1.0000000 NA NA NA NA NA NA NA 5.750000e-05 1.623710e-02 -0.2044030 -6.856400e-03 4.000000e-07 6.879800e-03 1.776770e-01 ▁▁▇▁▁
numeric SE 0 1.0000000 NA NA NA NA NA NA NA 1.307990e-02 9.031200e-03 0.0051174 6.041200e-03 8.963800e-03 1.773550e-02 9.209920e-02 ▇▂▁▁▁
numeric PVAL 0 1.0000000 NA NA NA NA NA NA NA 4.851647e-01 2.928095e-01 0.0000000 2.300001e-01 4.799997e-01 7.400005e-01 1.000000e+00 ▇▇▇▇▇
numeric PVAL_ztest 0 1.0000000 NA NA NA NA NA NA NA 4.851627e-01 2.927843e-01 0.0000000 2.278212e-01 4.802877e-01 7.390792e-01 9.999993e-01 ▇▇▇▇▇
numeric AF 0 1.0000000 NA NA NA NA NA NA NA 2.275319e-01 2.593234e-01 0.0051350 2.436600e-02 1.117050e-01 3.597560e-01 9.948650e-01 ▇▂▁▁▁
numeric AF_reference 84342 0.9902966 NA NA NA NA NA NA NA 2.273643e-01 2.513125e-01 0.0000000 2.216450e-02 1.283950e-01 3.570290e-01 1.000000e+00 ▇▂▂▁▁

Head and tail

CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
1 49298 rs200943160 T C -0.0053940 0.0094482 0.5700002 0.5680680 0.623975 0.7821490 NA
1 54676 rs2462492 C T -0.0045611 0.0093925 0.6300007 0.6272435 0.399195 NA NA
1 86028 rs114608975 T C -0.0027836 0.0148828 0.8499999 0.8516334 0.104010 0.0277556 NA
1 91536 rs6702460 G T -0.0117338 0.0092405 0.2000000 0.2041477 0.456260 0.4207270 NA
1 234313 rs8179466 C T -0.0070588 0.0180646 0.6999999 0.6959776 0.074925 NA NA
1 534192 rs6680723 C T 0.0001833 0.0105618 0.9900000 0.9861525 0.240477 NA NA
1 546697 rs12025928 A G -0.0031763 0.0131049 0.8100000 0.8084902 0.912594 NA NA
1 693731 rs12238997 A G 0.0095930 0.0087935 0.2800000 0.2753090 0.117168 0.1417730 NA
1 705882 rs72631875 G A 0.0085542 0.0128132 0.5000000 0.5043811 0.067988 0.0315495 NA
1 706368 rs55727773 A G 0.0088581 0.0065128 0.1700000 0.1737985 0.513917 0.2751600 NA
CHROM POS ID REF ALT EFFECT SE PVAL PVAL_ztest AF AF_reference N
22 51219006 rs28729663 G A 0.0030878 0.0078793 0.6999999 0.6951437 0.136721 0.2052720 NA
22 51219387 rs9616832 T C 0.0041374 0.0102857 0.6899999 0.6874995 0.072286 0.0654952 NA
22 51219704 rs147475742 G A 0.0185495 0.0137622 0.1800002 0.1777039 0.041090 0.0473243 NA
22 51221190 rs369304721 G A 0.0130044 0.0138158 0.3500000 0.3465664 0.048574 NA NA
22 51221731 rs115055839 T C 0.0043681 0.0102882 0.6700003 0.6711488 0.071794 0.0625000 NA
22 51222100 rs114553188 G T 0.0030116 0.0120129 0.8000000 0.8020502 0.054259 0.0880591 NA
22 51223637 rs375798137 G A 0.0028183 0.0120757 0.8200001 0.8154610 0.053868 0.0788738 NA
22 51229805 rs9616985 T C 0.0048963 0.0103254 0.6400000 0.6353604 0.071671 0.0730831 NA
22 51232488 rs376461333 A G -0.0067647 0.0244187 0.7800007 0.7817568 0.019926 NA NA
22 51237063 rs3896457 T C -0.0013114 0.0062658 0.8300000 0.8342250 0.297838 0.2050720 NA

bcf preview

1   49298   rs10399793  T   C   .   PASS    AF=0.623975 ES:SE:LP:AF:ID  -0.00539397:0.0094482:0.244125:0.623975:rs10399793
1   54676   rs2462492   C   T   .   PASS    AF=0.399195 ES:SE:LP:AF:ID  -0.00456109:0.00939249:0.200659:0.399195:rs2462492
1   86028   rs114608975 T   C   .   PASS    AF=0.10401  ES:SE:LP:AF:ID  -0.0027836:0.0148828:0.0705811:0.10401:rs114608975
1   91536   rs6702460   G   T   .   PASS    AF=0.45626  ES:SE:LP:AF:ID  -0.0117338:0.0092405:0.69897:0.45626:rs6702460
1   234313  rs8179466   C   T   .   PASS    AF=0.074925 ES:SE:LP:AF:ID  -0.00705885:0.0180646:0.154902:0.074925:rs8179466
1   534192  rs6680723   C   T   .   PASS    AF=0.240477 ES:SE:LP:AF:ID  0.000183312:0.0105618:0.00436481:0.240477:rs6680723
1   546697  rs12025928  A   G   .   PASS    AF=0.912594 ES:SE:LP:AF:ID  -0.00317629:0.0131049:0.091515:0.912594:rs12025928
1   693731  rs12238997  A   G   .   PASS    AF=0.117168 ES:SE:LP:AF:ID  0.00959302:0.00879353:0.552842:0.117168:rs12238997
1   705882  rs72631875  G   A   .   PASS    AF=0.067988 ES:SE:LP:AF:ID  0.00855425:0.0128132:0.30103:0.067988:rs72631875
1   706368  rs12029736  A   G   .   PASS    AF=0.513917 ES:SE:LP:AF:ID  0.00885811:0.00651284:0.769551:0.513917:rs12029736
1   714596  rs149887893 T   C   .   PASS    AF=0.033778 ES:SE:LP:AF:ID  -0.00288296:0.0161977:0.0655015:0.033778:rs149887893
1   715265  rs12184267  C   T   .   PASS    AF=0.037473 ES:SE:LP:AF:ID  -0.00670531:0.0147294:0.187087:0.037473:rs12184267
1   715367  rs12184277  A   G   .   PASS    AF=0.037571 ES:SE:LP:AF:ID  -0.00744892:0.0146769:0.21467:0.037571:rs12184277
1   717485  rs12184279  C   A   .   PASS    AF=0.037251 ES:SE:LP:AF:ID  -0.00845948:0.0147835:0.244125:0.037251:rs12184279
1   717587  rs144155419 G   A   .   PASS    AF=0.01631  ES:SE:LP:AF:ID  -0.0227924:0.0231698:0.481486:0.01631:rs144155419
1   720381  rs116801199 G   T   .   PASS    AF=0.037847 ES:SE:LP:AF:ID  -0.00674138:0.0146137:0.19382:0.037847:rs116801199
1   721290  rs12565286  G   C   .   PASS    AF=0.037928 ES:SE:LP:AF:ID  -0.00572725:0.0145682:0.161151:0.037928:rs12565286
1   722670  rs116030099 T   C   .   PASS    AF=0.101448 ES:SE:LP:AF:ID  -0.00320979:0.0107614:0.113509:0.101448:rs116030099
1   723891  rs2977670   G   C   .   PASS    AF=0.95784  ES:SE:LP:AF:ID  0.0042014:0.0140174:0.119186:0.95784:rs2977670
1   724849  rs12126395  C   A   .   PASS    AF=0.031533 ES:SE:LP:AF:ID  0.0206408:0.0260869:0.366532:0.031533:rs12126395
1   725060  rs865924913 A   T   .   PASS    AF=0.0526   ES:SE:LP:AF:ID  -0.0035301:0.0207161:0.0655015:0.0526:rs865924913
1   726794  rs28454925  C   G   .   PASS    AF=0.037383 ES:SE:LP:AF:ID  -0.00542414:0.0146699:0.148742:0.037383:rs28454925
1   729632  rs116720794 C   T   .   PASS    AF=0.03771  ES:SE:LP:AF:ID  -0.00367693:0.0145439:0.09691:0.03771:rs116720794
1   729679  rs4951859   C   G   .   PASS    AF=0.841119 ES:SE:LP:AF:ID  -0.00585046:0.00759998:0.356547:0.841119:rs4951859
1   730087  rs148120343 T   C   .   PASS    AF=0.055988 ES:SE:LP:AF:ID  0.0147838:0.0123721:0.638272:0.055988:rs148120343
1   731718  rs58276399  T   C   .   PASS    AF=0.123152 ES:SE:LP:AF:ID  0.00862722:0.00834174:0.522879:0.123152:rs58276399
1   732989  rs369030935 C   T   .   PASS    AF=0.025602 ES:SE:LP:AF:ID  -0.00437161:0.020621:0.0809219:0.025602:rs369030935
1   734349  rs141242758 T   C   .   PASS    AF=0.122336 ES:SE:LP:AF:ID  0.00803066:0.00834768:0.468521:0.122336:rs141242758
1   736289  rs79010578  T   A   .   PASS    AF=0.133575 ES:SE:LP:AF:ID  0.0102268:0.00821015:0.677781:0.133575:rs79010578
1   736689  rs181876450 T   C   .   PASS    AF=0.01114  ES:SE:LP:AF:ID  0.035742:0.0299483:0.638272:0.01114:rs181876450
1   740284  rs61770167  C   T   .   PASS    AF=0.006317 ES:SE:LP:AF:ID  -0.003711:0.0365383:0.0362122:0.006317:rs61770167
1   752478  rs146277091 G   A   .   PASS    AF=0.037646 ES:SE:LP:AF:ID  -0.00595822:0.0143941:0.167491:0.037646:rs146277091
1   752566  rs3094315   G   A   .   PASS    AF=0.837053 ES:SE:LP:AF:ID  -0.00613416:0.00735541:0.39794:0.837053:rs3094315
1   752721  rs3131972   A   G   .   PASS    AF=0.836576 ES:SE:LP:AF:ID  -0.00620798:0.00734597:0.39794:0.836576:rs3131972
1   753405  rs3115860   C   A   .   PASS    AF=0.868582 ES:SE:LP:AF:ID  -0.00801559:0.00789238:0.508638:0.868582:rs3115860
1   753541  rs2073813   G   A   .   PASS    AF=0.131123 ES:SE:LP:AF:ID  0.0063437:0.00790815:0.376751:0.131123:rs2073813
1   754063  rs12184312  G   T   .   PASS    AF=0.038071 ES:SE:LP:AF:ID  -0.00758969:0.0141775:0.229148:0.038071:rs12184312
1   754105  rs12184325  C   T   .   PASS    AF=0.038314 ES:SE:LP:AF:ID  -0.00778349:0.0140914:0.236572:0.038314:rs12184325
1   754182  rs3131969   A   G   .   PASS    AF=0.867856 ES:SE:LP:AF:ID  -0.00802382:0.00787636:0.508638:0.867856:rs3131969
1   754192  rs3131968   A   G   .   PASS    AF=0.867961 ES:SE:LP:AF:ID  -0.00838719:0.00788039:0.537602:0.867961:rs3131968
1   754211  rs12184313  G   A   .   PASS    AF=0.038295 ES:SE:LP:AF:ID  -0.00797225:0.0141427:0.244125:0.038295:rs12184313
1   754334  rs3131967   T   C   .   PASS    AF=0.867877 ES:SE:LP:AF:ID  -0.00785082:0.00787618:0.49485:0.867877:rs3131967
1   754433  rs150578204 G   A   .   PASS    AF=0.005262 ES:SE:LP:AF:ID  0.0242962:0.0399593:0.267606:0.005262:rs150578204
1   754458  rs142682604 G   T   .   PASS    AF=0.005236 ES:SE:LP:AF:ID  0.0251324:0.0400565:0.275724:0.005236:rs142682604
1   754503  rs3115859   G   A   .   PASS    AF=0.836132 ES:SE:LP:AF:ID  -0.0060659:0.00733237:0.387216:0.836132:rs3115859
1   754629  rs10454459  A   G   .   PASS    AF=0.038314 ES:SE:LP:AF:ID  -0.00786371:0.0141621:0.236572:0.038314:rs10454459
1   754964  rs3131966   C   T   .   PASS    AF=0.836763 ES:SE:LP:AF:ID  -0.00621462:0.00735257:0.39794:0.836763:rs3131966
1   755240  rs181660517 T   G   .   PASS    AF=0.012805 ES:SE:LP:AF:ID  0.0242779:0.0267565:0.443698:0.012805:rs181660517
1   755435  rs184270342 T   G   .   PASS    AF=0.005548 ES:SE:LP:AF:ID  -0.0441839:0.0397426:0.568636:0.005548:rs184270342
1   755775  rs3131965   A   G   .   PASS    AF=0.838168 ES:SE:LP:AF:ID  -0.00677724:0.00745735:0.443698:0.838168:rs3131965